Toward the Structure of Dynamic Membrane-Anchored Actin Networks

Gerisch, G.; Weber, Igor

doi:10.4161/cam.1.3.4662

Cited by 6 publications

(2 citation statements)

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“…However, when Dictyostelium cells adhere to Petri dishes, tethers dominate the force curves. The pseudopodia-rich surface structure is thought to support tether formation (57). In this case, tethers can also reach lengths of several tens of micrometers and more (see Fig.…”

Section: Single Cell-to-cell Measurementsmentioning

confidence: 99%

Measuring Cell Adhesion Forces: Theory and Principles

Benoit

Selhuber‐Unkel²

2011

Methods in Molecular Biology

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Cell adhesion is an essential prerequisite for survival, communication, and navigation of cells in organisms. It is maintained by the organized binding of molecules from the cell membrane to the extracellular space. This chapter focuses on direct measurements of cellular binding strength at the level of single adhesion molecules. Using atomic force microscopy-based force measurements, adhesion strength can be monitored as a function of adhesion time and environmental conditions. In this way, cellular adhesion strategies like changes in affinity and avidity of adhesion molecules (e.g., integrins) are characterized as well as the molecular arrangement of adhesion molecules in the cell membrane (e.g., molecular clusters, focal adhesion spots, and linkage to the cytoskeleton or tether). Some prominent values for the data evaluation are presented as well as constraints and preparative techniques for successful cell adhesion force experiments.

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Section: Single Cell-to-cell Measurementsmentioning

confidence: 99%

Measuring Cell Adhesion Forces: Theory and Principles

Benoit

Selhuber‐Unkel²

2011

Methods in Molecular Biology

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“…Electron microscopy (EM) serves as powerful tool to visualize the organization of actin networks but often comes with technical caveats. Cryo-EM allows the vitrification of the specimen and visualization of cellular structures in its native context but it is limited by the thickness of the sample to 600 nm (Gerisch and Weber, 2007). With the newly developed in situ cryo-electron tomography (cryo-ET) combined with cryo-focused ion beam (cryo-FIB) milling of frozen-hydrated cells, it is now possible to visualize subcellular structures in the native environments of thicker cells.…”

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confidence: 99%

Deconstructing Actin Waves

2019

Structure

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The Model Organism Dictyostelium discoideum

Bozzaro

2013

Methods in Molecular Biology

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Much of our knowledge of molecular cellular functions is based on studies with a few number of model organisms that were established during the last 50 years. The social amoeba Dictyostelium discoideum is one such model, and has been particularly useful for the study of cell motility, chemotaxis, phagocytosis, endocytic vesicle traffic, cell adhesion, pattern formation, caspase-independent cell death, and, more recently, autophagy and social evolution. As nonmammalian model of human diseases D. discoideum is a newcomer, yet it has proven to be a powerful genetic and cellular model for investigating host-pathogen interactions and microbial infections, for mitochondrial diseases, and for pharmacogenetic studies. The D. discoideum genome harbors several homologs of human genes responsible for a variety of diseases, -including Chediak-Higashi syndrome, lissencephaly, mucolipidosis, Huntington disease, IBMPFD, and Shwachman-Diamond syndrome. A few genes have already been studied, providing new insights on the mechanism of action of the encoded proteins and in some cases on the defect underlying the disease. The opportunities offered by the organism and its place among the nonmammalian models for human diseases will be discussed.

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Toward the Structure of Dynamic Membrane-Anchored Actin Networks

Cited by 6 publications

References 30 publications

Measuring Cell Adhesion Forces: Theory and Principles

Measuring Cell Adhesion Forces: Theory and Principles

Deconstructing Actin Waves

The Model Organism Dictyostelium discoideum

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