Toward the understanding of the role of CDC48, a major component of the protein quality control, in plant immunity

Abstract

The evolutionally conserved chaperone-like protein CDC48 (cell division cycle 48) is a major component of ubiquitin-dependent protein degradation pathways in animal and yeast and, more generally, of the protein quality control machinery. In plants, CDC48 plays essential regulatory functions in development and the possibly that it contributes to protein degradation through the ubiquitin-proteasome system (UPS) and the endoplasmic reticulum-associated protein degradation (ERAD) system has been reported. In this review we described recent findings highlighting a role for CDC48 in plant immunity. First data indicated that CDC48 is S-nitrosylated in plant cells undergoing an immune response, regulates the turnover of immune receptors and mediates the degradation of viral proteins.Furthermore its overexpression was associated to an exacerbated hypersensitive-like cell death. We also designed and reported here the first CDC48 interactome. The corresponding data confirms the closed interaction of CDC48 with components of the UPS and shed light on its putative regulatory function of S-adenosyl-methionine synthesis and metabolism. More generally, these investigations further support the concept that plant cells facing pathogen attack finely regulate the protein quality control machinery. Highlights-The chaperone-like protein CDC48 is a component of the ubiquitin-proteasome system -CDC48 contributes to the turnover of immune receptors -CDC48 is mobilized by cells facing an immune response -Overproduction of CDC48 leads to exacerbated cell death during the immune response -The A. thaliana CDC48 interactome highlights new functions of CDC48 Keywords

Comparative Transcriptome Analysis of Two Cucumber Cultivars with Different Sensitivity to Cucumber Mosaic Virus Infection

“…The proteins with ubiquitin-regulating region X (UBX) participate in Cdc48/p97-substrate binding, as well as in time and space regulation its activity [69]. It probably substantially contributes to plant immunity by precise control of produced proteins in virus-attacked cells [70]. On the other hand, some authors presume positive influence of the ubiquitin-proteasome system on infection by (+)ssRNA viruses by regulation of concentration of particular viral proteins [71].…”

Identification of genes associated with productivity traits and salinity tolerance from activation tagged lines of rice

“…AT plants of A10- DS-RFP6 exhibited substantial 16.68-fold increase in CDC48 expression levels in comparison to WT plants. It was reported that the CDC48 could play a crucial regulatory role in plant development besides protein degradation through ubiquitin-proteasome system (UPS) and endoplasmic reticulum-associated protein degradation (ERAD) system (Begue et al ., 2019). Shi et al ., (2019) observed that the transgenic rice overexpressing Os CDC48 promoted increased tiller number and grain yield.…”

Cross Kingdom Immunity: The Role of Immune Receptors and Downstream Signaling in Animal and Plant Cell Death

“…Investigations of CDC48 function in plant immunity demonstrated that the tobacco CDC48 isoform rapidly accumulates in its hexameric active structure in tobacco cells exposed to cryptogein (165). A screening for its binding partners allowed to the identification of key regulators of the redox status, including cytosolic ascorbate peroxidase (cAPX) a pivotal enzyme for ROS removal (165,166). In CDC48-overexpressing tobacco cells, the activity of cAPX was impaired, leading to severe decrease in the cell capacity to respond to oxidative stress (167).…”