Background:Organizations spend an extensive amount of time and money on training in order to promote employees' learning, development, and job-related competencies. This study examined the influence of trainee characteristics of the dental practitioners attending the continuing medical education (CME) on"dental pharmacology and patient safety" and their effect on acquired knowledge. Methods: The trainee characteristics of the dental practitioners were conceptualized into four dimensions: motivation to learn, reaction to training, expectations from training, and organizational commitment. A questionnaire containing 20 items related to measuring the levels of knowledge acquired from training were included. With SPSS ver. 21, central tendencies and dispersion, Pearson correlation, and simple linear regression were measured (p<0.05). Results: The results of this study found a positive correlation between the independent variables. Through linear regression, a positive correlation between the independent variables and acquired knowledge was discovered. Conclusion: A combination of personality traits is vital for dental professionals to excel in dental training programs.