Towards a Clinical Application of Freeze-Dried Human Platelets

Dry storage of sperm: applications in primates and domestic animals

“…Several strategies to improve the mammalian cell's ability to take up trehalose have been reported, including a genetic engineering approach, in which a Staphylococcus α-haemolysin was used to porate mammalian fibroblasts and keratinocytes for trehalose uptake (Eroglu et al 2000), trehalose microinjection (Eroglu et al 2002) and thermal poration (Beattie et al 1997). It has recently been shown that mesenchymal stem cells can take up trehalose from the external environment by fluid phase endocytosis, reaching internal trehalose concentrations in the range 20-30 mm (Oliver et al 2004), which has been shown to protect human platelets during freeze-drying and storage (Wolkers et al 2001(Wolkers et al , 2002.…”

“…The dehydrated storage of cells represents an alternative to cryopreservation and has already been shown to be effective for the storage of human blood platelets at room temperature for up to 2 years, during which time recovery and response to thrombin remained essentially unchanged (Wolkers et al 2001(Wolkers et al , 2002. Efforts to dry nucleated cells have also been reported (Guo et al 2000;Bloom et al 2001;Gordon et al 2001;Puhlev et al 2001;Acker et al 2002;Bhowmick et al 2003), but achieving consistent results of highly viable, physiologically active cells following dehydration to low water content remains elusive.…”

“…The addition of DMSO failed to enhance trehalose uptake. Subsequent studies used these loading conditions to obtain intracellular trehalose concentrations that are similar to those seen in trehalose-loaded human platelets (Wolkers et al 2002). Sperm were then loaded with trehalose and dried in a vacuum oven at ambient temperature for up to 150 min in order to assess the temperature-independent ability for trehaloseloaded and control sperm to undergo drying and to determine water content by gravimetric methods.…”

Trehalose Can Improve Platelet Aggregation Versus Collagen Agonist During Lyophilized Platelet Preparation

“…In 2001, Wolkers et alfreeze-dried platelets and made the recovery rate of 85%, with the protection of trehalose 8 . And they developed a freeze-drying method of platelets of large volume 9,10 . In 2007 Zhou et al optimized the freeze drying protocol of human platelets with respect to lyoprotectant, cooling rate and cell concentration 11 .…”

Yeast Cell Death During the Drying and Rehydration Process

“…Attempts to enhance the desiccation tolerance of cells first focused on plant cells and seeds of agricultural significance, as the availability of water is one of the main parameters that limits plant productivity (Bartels et al, 2001). More recently, lyophilisation and other dehydrationbased technologies have been explored by a number of groups for the purpose of cell and tissue preservation (Liang et al, 2002;Wolkers et al, 2002;Elliott et al, 2006). Furthermore, active dry yeast is commonly used in the food industry for the production of beer, wine and bread.…”