Towards a fingerprint method covering the immunological genome

Gomolka, Maria; Schwaiger, W.; Weyers, E.; Epplen, Cornelia; Buitkamp, Johannes; Epplen, Jörg T.

doi:10.1002/elps.11501301127

Cited by 3 publications

(1 citation statement)

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“…Genes encoding these molecules as well as a number of effector molecules are prime candidates for the investigation of genetic variation in resistance to infections in animals and man. The application of numerous markers in the vicinity of these genes (the immunological genome) leads to an approach called immunoprinting in man (E pplen 1992; G omolka et al 1992). In domestic animals only a few of the potentially involved loci have been characterized genetically (B uitkamp et al 1993a).…”

Section: Introductionmentioning

confidence: 99%

Major histocompatibility and T‐cell receptor genes in Artiodactyls: characterization, polymorphism and genetic resistance to a helmintic infection

Buitkamp

Epplen

1996

J Animal Breeding Genetics

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Section: Introductionmentioning

confidence: 99%

Major histocompatibility and T‐cell receptor genes in Artiodactyls: characterization, polymorphism and genetic resistance to a helmintic infection

Buitkamp

Epplen

1996

J Animal Breeding Genetics

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Towards covering immunological genes with highly informative markers: A trans-species approach

Buitkamp

Schwaiger²,

Epplen³

et al. 1993

DNA Fingerprinting: State of the Science

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Typing of artiodactyl MHC‐DRB genes with the help of intronic simple repeated DNA sequences

et al. 1993

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An efficient oligonucleotide typing method for the highly polymorphic MHC-DRB genes is described for artiodactyls like cattle, sheep and goat. By means of the polymerase chain reaction, the second exon of MHC-DRB is amplified as well as part of the adjacent intron containing a mixed simple repeat sequence. Using this primer combination we were able to amplify the MHC-DRB exons 2 and adjacent introns from all of the investigated 10 species of the family of Bovidae and giraffes. Therefore, the DRB genes of novel artiodactyl species can also be readily studied. Oligonucleotide probes specific for the polymorphisms of ungulate DRB genes are used with which sequences differing in at least one single base can be distinguished. Exonic polymorphism was found to be correlated with the allele lengths and the patterns of the repeat structures. Hence oligonucleotide probes specific for different simple repeats and polymorphic positions serve also for typing across species barriers. The strict correlation of sequence length and exonic polymorphism permits a preselection of specific oligonucleotides for hybridization. Thus more than 20 alleles can already be differentiated from each of the three species.

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Towards a fingerprint method covering the immunological genome

Abstract: The principle of a novel typing techniques is described for screening with high efficiency the “immunological genome” by using various intronic and exonic oligonucleotide hybridization probes simultaneously or subsequently.

Cited by 3 publications

References 5 publications

Major histocompatibility and T‐cell receptor genes in Artiodactyls: characterization, polymorphism and genetic resistance to a helmintic infection

Major histocompatibility and T‐cell receptor genes in Artiodactyls: characterization, polymorphism and genetic resistance to a helmintic infection

Towards covering immunological genes with highly informative markers: A trans-species approach

Typing of artiodactyl MHC‐DRB genes with the help of intronic simple repeated DNA sequences

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