In real-time multimedia applications, the most problems are how to store, process, and transport the multimedia data with cloud, how to optimize the cloud platform to satisfy the user demand, as well as how to provide the quality of service (QoS) guarantees by combining cloud platform and wireless sensor networks (WSNs). To overcome these issues, the multimedia cloud was designed according to the characteristics of group of pictures (GOP), determining degree, and video frame density. The opportunistic dynamic multimedia cloud platform was proposed on the basis of the variation of packet error rate, decodable frame ratio and peak signal-to-noise ratio (PSNR) with channel quality. Then, the optimal multi-relay hierarchical cooperative multimedia transmission scheme was designed and implemented by reconstructing the cooperative multimedia stream based on the framing of GOP. For enhancing the multimedia cloud computing efficiency and decreasing the complexity of encoding and decoding of multimedia stream, we combined the opportunistic dynamic multimedia cloud platform and cooperative multimedia stream, which can also enhance the resources utilization of WSNs. Finally, the QoS guarantee protocol with the combination of opportunistic dynamic multimedia cloud platform and cooperative multimedia stream for WSNs (ODM-CM-WSN) was presented. Especially, the layered architectural model for multimedia applications and the opportunistic cooperative multimedia cloud service model were developed. The mathematical and simulation results demonstrate that the proposed QoS guarantee protocol can satisfy the various requirements of multimedia applications and provide reliable and efficient cloud services with WSNs.