Towards a knowledge-based correction of iron chlorosis

Vázquez, Saúl; Rellán‐Álvarez, Rubén; El-Jendoubi, Hamdi; Abadı́a, Anunciación; Álvarez‐Fernández, Ana; López‐Millán, Ana Flor

doi:10.1016/j.plaphy.2011.01.026

Cited by 302 publications

(224 citation statements)

References 141 publications

Supporting

Mentioning

217

Contrasting

Unclassified

Order By: Relevance

“…In particular, plants growing on calcareous soils are at risk of Fe deficiency because although ferric ions are often abundant in these soils they are largely unavailable to plant roots, owing to the extremely low solubility of ferric compounds. Therefore, mechanisms for the avoidance or correction of Fe deficiency in plants have been intensively studied [6][7][8][9][10]. However, it is not yet known why or how some plant species secrete flavins from their roots under conditions of Fe deficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Confocal fluorescent microscopy showed that riboflavin was mainly localized in the epidermis and cortex of the root tip and, at the cellular level, in the apoplast. The expressions of three genes involved in the de novo biosynthesis of riboflavin (GTP cyclohydrolase II/3,6, riboflavin synthase) were compared between Fe-starved and Fe-replete roots over a time course of 7 days, using RT-PCR. All three genes were found to be highly expressed over the period 1-7 days in the roots cultured under Fe deficiency.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency

Higa

Khandakar

Mori

et al. 2012

Plant Physiology and Biochemistry

View full text Add to dashboard Cite

Riboflavin secretion by Hyoscyamus albus hairy roots under Fe deficiency was examined to determine where riboflavin is produced and whether production occurs via an enhancement of riboflavin biosynthesis or a stimulation of flavin mononucleotide (FMN) hydrolysis. Confocal fluorescent microscopy showed that riboflavin was mainly localized in the epidermis and cortex of the root tip and, at the cellular level, in the apoplast. The expressions of three genes involved in the de novo biosynthesis of riboflavin (GTP cyclohydrolase II/3,6, riboflavin synthase) were compared between Fe-starved and Fe-replete roots over a time course of 7 days, using RT-PCR. All three genes were found to be highly expressed over the period 1-7 days in the roots cultured under Fe deficiency. Since riboflavin secretion began to be detected only from 3 days, there was a lag phase observed between the increased transcript accumulations and riboflavin secretion. To determine whether FMN hydrolysis might contribute to the riboflavin secretion in Fe-deficient root cultures, FMN hydrolase activity was determined and was found to be substantially increased after 3 days, when riboflavin secretion became detectable. These results suggested that not only de novo riboflavin synthesis but also the hydrolysis of FMN contributes to riboflavin secretion under conditions of Fe deficiency. Respiration activity was assayed during the time-course, and was also found to be enhanced after 3 days under Fe deficiency, suggesting a possible link with riboflavin secretion. On the other hand, several respiratory inhibitors were found not to affect riboflavin synthase transcript accumulation.Key words: Hyoscyamus albus; hairy roots; iron deficiency; riboflavin secretion; riboflavin biosynthesis; FMN hydrolase; respiration. RibA: GTP cyclohydrolase II · RibB: 3,4-dihydroxy-2-butanone 4-phosphate synthase · RibC: 6,7-dimethyl-8-ribityllumazine synthase · RibD: riboflavin synthase

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency

Higa

Khandakar

Mori

et al. 2012

Plant Physiology and Biochemistry

View full text Add to dashboard Cite

show abstract

“…After the addition of NH 4 OH (0.2 ml/ml sample) in order to precipitate the titanium-peroxide complex, samples were centrifuged at 10 000 g for 5 min. The resulting pellet was washed five times in acetone and re-suspended in 2N H 2 SO 4 . The absorbance of the solution was spectrophotometrically determined at 415 nm against a blank containing H 2 O 2 instead of sample extract.…”

Section: Hydrogen Peroxide In Leavesmentioning

confidence: 99%

“…This can compromise the growth and development of several leguminous species, which commonly face Fe deficiency-induced chlorosis. The presence of a wide variability of plant responses to low soil Fe availability has been reported, both among legumes and among other species [3,4]. Several environmental conditions are reported to induce oxidative stress in plants [5].…”

Section: Introductionmentioning

confidence: 99%

Implication of Antioxidant Defence in Tolerance to Fe Deficiency of Pisum Sativum Leaves

Jelali¹

2017

JABB

View full text Add to dashboard Cite

The intend of this work was to investigate whether some leaf parameters involved in the antioxidant mechanisms were differently expressed in two pea (Pisum sativum L.) cultivars characterized by different tolerance to Fe deficiency: cv. Kelvedon, tolerant and cv. Lincoln, susceptible. Both cultivars were grown in a full strength nutrient solution (C), in the absence of Fe (direct deficiency, DD) or in the presence of Fe and bicarbonate (induced deficiency, ID) for 12 days. Our results show that peroxidase activity and isoform expression increased in Kelvedon in both treatments, while it decreased in Lincoln plants grown in the presence of bicarbonate, suggesting the involvement of this activity improvement in Fe deficiency tolerance of pea plants. Interestingly, while only slight differences were reported between both cultivars under DD, the adaptation of Kelvedon under ID seems to be related, at least in part, to its greater ability to enhance the antioxidant defense at leaf level.

show abstract

“…Vivianite [Fe 3 (PO 4 ) 2 · 8H 2 O] is a Fe salt poorly soluble in calcareous media, which can be solubilised by compounds with affinity for Fe, such as citrate and diethylenetriamine pentaacetic acid (DTPA) (De Santiago et al, 2008a). Therefore, it can act as a slow-release Fe fertilizer which can be dissolved by the action of carboxylates compounds secreted by roots, resulting in a high Fe bioavailability (Abadía et al, 2011). Also, dissolution is enhanced by the phosphate adsorption of plant roots, which decreases the activity of this anion in the soil solution (Eynard et al, 1992;Rosado et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of mixtures of vivianite and organic materials in preventing iron chlorosis in strawberry

Santiago¹,

Carmona²,

Quintero³

et al. 2013

Span. j. agric. res.

View full text Add to dashboard Cite

La aplicación de sales de hierro con materia orgánica de diferentes fuentes ha demostrado ser efectiva en la prevención de la clorosis férrica. El principal objetivo de este trabajo fue estudiar la efectividad de distintas fuentes de Fe formadas a partir de la mezcla de sustancias húmicas o compost con vivianita, en la prevención de este desorden nutricional en fresa (Fragaria x ananassa cv Camarosa). Para ello, se dispuso en un invernadero un experimento diseñado en bloques al azar, con 4 repeticiones (3 plantas por repetición) y un solo factor (la fuente de Fe), usando un medio calcáreo como soporte para las plantas. Las fuentes de Fe estudiadas fueron: (i) testigo sin Fe, (ii) testigo con una fuente efectiva de Fe en medios calcáreos (EDDHA-Fe, 0.1g kg-1), (iii) vivianita (1 g kg-1 medio, dosis recomendada), (iv) vivianita (1 g kg-1) + sustancias húmicas (–SH– 0.06 g kg-1), (v) vivianita (0.5 g kg-1) + SH (0.06 g kg-1) y (vi) una mezcla de corcho compostado y vivianita en una relación 6:1 en peso (CORVIV) aplicada a una dosis de 6 g kg-1 medio. Todas las fuentes de Fe fueron efectivas incrementando las medidas de clorofila SPAD cuando se compararon con el testigo sin Fe. Los tratamientos basados en vivianita no produjeron diferencias significativas respecto a aquellos obtenidos con EDDHA-Fe. Sin embargo, solo el CORVIV no mostró diferencias significativas con respecto al quelato de Fe en cuanto a la producción de materia seca (MS), el área foliar y el contenido en Fe total presente en la parte aérea. Las sustancias húmicas y la vivianita aplicadas en la dosis recomendada (1 g kg–1) incrementaron la producción de MS en las plantas con respecto a la vivianita sin sustancias húmicas. Además la vivianita aplicada sin mezclar con materia orgánica resultó comportarse de forma similar a la dosis inferior de vivianita (0.5 g kg–1) aplicada junto con sustancias húmicas. Se podría concluir que las fuentes de materia orgánica estudiadas incrementaron la eficiencia de la vivianita en la prevención de la clorosis en fresa, especialmente cuando la vivianita se enriqueció con compost de corcho, siendo tan efectiva como el EDDHA-Fe.

show abstract

Towards a knowledge-based correction of iron chlorosis

Cited by 302 publications

References 141 publications

Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency

Increased de novo riboflavin synthesis and hydrolysis of FMN are involved in riboflavin secretion from Hyoscyamus albus hairy roots under iron deficiency

Implication of Antioxidant Defence in Tolerance to Fe Deficiency of Pisum Sativum Leaves

Effectiveness of mixtures of vivianite and organic materials in preventing iron chlorosis in strawberry

Contact Info

Product

Resources

About