Towards a multi-electrode array (MEA) system for patterned neural networks

Yao, Yi Ping; Gnerlich, Markus; Perry, Susan; Tatić-Lučić, Svetlana

doi:10.1016/j.proche.2009.07.082

Cited by 6 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mostly, well-defined electrode arrays were fabricated on silicon substrate to generate dielectrophoretic (DEP) forces, and the desired number of neurons were immobilized onto recording electrodes [ 65 ]. Jing et al [ 66 ] used SAM to create hydrophobic and hydrophilic areas on MEAs, which would lead a patterned neuronal network on the substrate. Spontaneous electrical signals were detected from the networks of GT1-7 neurons and primary mouse hippocampal neurons.…”

Section: Applications In Biochemical Monitoring Of Living Cellsmentioning

confidence: 99%

Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro

Wang

et al. 2012

Biosensors

View full text Add to dashboard Cite

Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

show abstract

Section: Applications In Biochemical Monitoring Of Living Cellsmentioning

confidence: 99%

Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro

Wang

et al. 2012

Biosensors

View full text Add to dashboard Cite

show abstract

“…9(c). The detailed microfabrication process has been described in detail elsewhere (Jing et al 2009). Briefly, a thin film of Cr/Au/ Cr is patterned on top of a glass wafer, which forms electrodes and interconnects.…”

Section: Patterned Neural Networkmentioning

confidence: 99%

Precise cell patterning using cytophobic self-assembled monolayer deposited on top of semi-transparent gold

Perry

Tatić-Lučić

2010

Biomed Microdevices

Self Cite

View full text Add to dashboard Cite

This paper reports a simple and effective method for cell patterning by using a self-assembled monolayer (SAM)-treated glass surface which is surrounded by semi-transparent gold coated with another type of SAM. Specifically, a hydrophobic SAM, derived from 1-hexadecanethiol (HDT), was coated on the gold surface to prevent cell growth, and a hydrophilic SAM, derived from 3-trimethoxysilyl propyl-diethylenetriamine (DETA), was coated on the exposed glass surface to promote cell growth. The capabilities of this technique are as follows: 1) single-cell resolution, 2) easy alignment of the cell patterns to the structures already existing on the substrate, 3) visualization and verification of the predefined cytophobic/cytophilic pattern prior to cell growth, and 4) convenient monitoring cell growth at the same location for an extended long term period of time. Whereas a number of earlier techniques have demonstrated the single cell resolution, or visualization and verification of the cytophobic/cytophilic patterns prior to cell growth, we believe that our technique is unique in possessing all of these beneficial qualities at the same time. The distinguishing characteristic of our technique is, however, that the use of semi-transparent Cr/Au film allows for convenient brightfield pattern visualization and offers an advantage over previously developed methods which require fluorescent imaging. We have successfully demonstrated the patterning of four different kinds of cells using this technique: immortalized mouse hypothalamic neurons (GT1-7), mouse osteoblast cells (MC3T3), mouse fibroblast cells (NIH3T3) and primary rat hippocampal neurons. This study was performed with a specific ultimate application-the creation of a multi electrode array (MEA) with predefined localization of cell bodies on top of the electrodes, as well as predefined patterns for cell extensions to grow in between the electrodes. With that goal in mind, we have also determined critical parameters for patterning of each of these cell types, such as the minimum size of a cell-adherent island for exclusively anchoring one cell or two cells, as well as the width of the cytophilic pathway between two islands that enables cell extensions to grow, while preventing the anchoring of the cell bodies. Additionally, we have provided statistical analysis of the occupancy for various sizes and shape of cell-anchoring islands. As demonstrated here, we have developed a novel and reliable cell patterning technique, which can be utilized in various applications, such as biosensors or tissue engineering.

show abstract

“…For studying the nervous system, various methods have been presented. One of the most commonly applied methods is multi-electrode arrays which are used to study the function of the cell and the neural tissue 1–3 .…”

Section: Introductionmentioning

confidence: 99%

Gold-Plated Electrode with High Scratch Strength for Electrophysiological Recordings

Vafaiee

Vossoughi

Mohammadpour

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Multi electrode arrays (MEA) have been exploited in different electrophysiological applications. In neurological applications, MEAs are the vital interfaces between neurons and the electronic circuits with dual role; transmitting electric signal to the neurons and converting neural activity to the electric signal. Since the performance of the electrodes has a direct effect on the quality of the recorded neuronal signal, as well as the stimulation, the true choice of electrode material for MEA is crucial. Gold is one of the best candidates for fabrication of MEAs due to its high electrical conductivity, biocompatibility and good chemical stability. However, noble metals such as gold do not adhere well to the glass substrate. Consequently while exposing to the water, gold films are damaged, which impose limitations in the exploiting of gold thin films as the electrode. In this paper, a simple and cost effective method for the fabrication of gold electrode arrays is proposed. Using various mechanical (adhesion test and scratch strength), morphological (AFM and SEM) and electrochemical methods, the fabricated electrodes are characterized. The results show that the fabricated electrode arrays have significantly high scratch strength and stability within the aqueous medium. In addition, the electrical properties of the electrodes have been improved. The proposed electrodes have the potential to be exploited in other applications including electronics, electrochemistry, and biosensors.

show abstract

Towards a multi-electrode array (MEA) system for patterned neural networks

Cited by 6 publications

References 7 publications

Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro

Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro

Precise cell patterning using cytophobic self-assembled monolayer deposited on top of semi-transparent gold

Gold-Plated Electrode with High Scratch Strength for Electrophysiological Recordings

Contact Info

Product

Resources

About