Towards a Multi-Omics of Male Infertility

Wagner, Ana Ogrinc; Turk, Aleksander; Kunej, Tanja

doi:10.5534/wjmh.220186

Cited by 28 publications

(15 citation statements)

References 127 publications

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“…The presence of miRNAs in testis, epididymis, sperm cells, seminal plasma, and extracellular vesicles (ie , exosomes and microvesicles) and the known functions associated with these molecules suggest that deregulation of their expression may result in alterations in spermatogenesis and embryogenesis [ 39 ]. Spermatogenesis is a highly complex and regulated process that produces millions of sperm every day and miRNAs play an important role in spermatogenesis during the mitotic and meiotic phases of spermatogenesis through the regulation of targeted gene expression [ 40 , 41 ]. Such perturbations could have the potential to give rise to various forms of infertility.…”

Section: Discussionmentioning

confidence: 99%

Normospermic Patients Infected With Ureaplasma parvum: Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p

Galdiero,

Trotta,

Schettino

et al. 2024

PAI

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Background: Ureaplasma parvum (UP) is a causative agent of non-gonococcal urethritis, involved in the pathogenesis of prostatitis and epididymitis, and it could impair human fertility. Although UP infection is a frequent cause of male infertility the study evidence assessing their prevalence and the association in patients with infertility is still scarce. The molecular processes leading to defects in spermatozoa quality are not completely investigated. MicroRNAs (miRNAs) have been extensively reported as gene regulatory molecules on post-transcriptional levels involved in various biological processes such as gametogenesis, embryogenesis, and the quality of sperm, oocyte, and embryos. Methods: Therefore, the study design was to demonstrate that miRNAs in body fluids like sperm could be utilized as non-invasive diagnostic biomarkers for pathological and physiological conditions such as infertility. A post-hoc bioinformatics analysis was carried out to predict the pathways modulated by the miRNAs dysregulated in the differently motile spermatozoa. Results: Here it is shown that normospermic patients infected by UP had spermatozoa with increased quantity of superoxide anions, reduced expression of miR-122-5p, miR-34c-5, and increased miR-141-3p compared with non-infected normospermic patients. This corresponded to a reduction of sperm motility in normospermic infected patients compared with normospermic non-infected ones. A target gene prediction presumed that an essential role of these miRNAs resided in the regulation of lipid kinase activity, accounting for the changes in the constitution of spermatozoa membrane lipids caused by UP. Conclusions: Altogether, the data underline the influence of UP on epigenetic mechanisms regulating spermatozoa motility.

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Section: Discussionmentioning

confidence: 99%

Normospermic Patients Infected With Ureaplasma parvum: Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p

Galdiero,

Trotta,

Schettino

et al. 2024

PAI

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“…Infertility affects around 30% of couples worldwide, and treatments can be stressful, intrusive, and expensive ( Wagner et al, 2023 ). Metagenomics is enhancing our knowledge about the composition and role of microbiota in human physiology and pathology.…”

Section: Metagenomic Studies In Mammals’ Semenmentioning

confidence: 99%

Mammals’ sperm microbiome: current knowledge, challenges, and perspectives on metagenomics of seminal samples

et al. 2023

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Bacterial growth is highly detrimental to sperm quality and functionality. However, during the last few years, using sequencing techniques with a metagenomic approach, it has been possible to deepen the study of bacteria-sperm relationships and describe non-culturable species and synergistic and antagonistic relationships between the different species in mammalian animals. We compile the recent metagenomics studies performed on mammalian semen samples and provide updated evidence to understand the importance of the microbial communities in the results of sperm quality and sperm functionality of males, looking for future perspectives on how these technologies can collaborate in the development of andrological knowledge.

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“…Any possible identified biomarkers of infertility can be used for diagnosis and prediction. Additionally, metabolomics can assist in evaluating the efficacy of infertility treatment drugs by analyzing their effects on the metabolic spectrum and predicting treatment outcomes ( 15 ). Overall, metabolomics has significant value in screening, identifying, diagnosing, and evaluating infertility treatment ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Application and discoveries of metabolomics and proteomics in the study of female infertility

Shi,

Wu,

et al. 2024

Front. Endocrinol.

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IntroductionFemale infertility is defined as the absence of clinical pregnancy after 12 months of regular unprotected sexual intercourse.MethodsThis study employed metabolomics and proteomics approaches to investigate the relationship between metabolites and proteins and female infertility. The study used metabolomics and proteomics data from the UK Biobank to identify metabolites and proteins linked to infertility.ResultsThe results showed that GRAM domain-containing protein 1C and metabolites fibrinogen cleavage peptides ADpSGEGDFXAEGGGVR and 3-Hydroxybutyrate had a positive correlation with infertility, whereas proteins such as Interleukin-3 receptor subunit alpha, Thrombospondin type-1 domain-containing protein 1, Intestinal-type alkaline phosphatase, and platelet and endothelial cell adhesion molecule 1 exhibited a negative correlation. These findings provide new clues and targets for infertility diagnosis and treatment. However, further research is required to validate these results and gain a deeper understanding of the specific roles of these metabolites and proteins in infertility pathogenesis.DiscussionIn conclusion, metabolomics and proteomics techniques have significant application value in the study of infertility, allowing for a better understanding of the biological mechanisms underlying infertility and providing new insights and strategies for its diagnosis and treatment. These research findings provide a crucial biological mechanistic basis for early infertility screening, prevention, and treatment.

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Towards a Multi-Omics of Male Infertility

Cited by 28 publications

References 127 publications

Normospermic Patients Infected With Ureaplasma parvum: Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p

Normospermic Patients Infected With Ureaplasma parvum: Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p

Mammals’ sperm microbiome: current knowledge, challenges, and perspectives on metagenomics of seminal samples

Application and discoveries of metabolomics and proteomics in the study of female infertility

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