Towards a Regional Registry of Extended Typed Blood Donors: Molecular Typing for Blood Group, Platelet and Granulocyte Antigens

Portegys, Jan; Rink, Gabi; Bloos, Pia; Scharberg, Erwin A.; Klüter, Harald; Bugert, Peter

doi:10.1159/000493555

Cited by 18 publications

(23 citation statements)

References 35 publications

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“…Among these techniques, real‐time PCR has been highlighted because of the ease of execution; automation potential; quickness to release results; and because the whole process of amplification and detection is performed in a closed system, minimising the risk of contamination . Thus, real‐time PCR has been increasingly used for inference of blood groups, including for rare blood donor identification …”

Section: Discussionmentioning

confidence: 99%

The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self‐reported ancestry

Muniz

Silva

Ferraz

et al. 2019

Transfusion Medicine

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Objectives: The present study aimed to develop strategies for genotyping DO*HY (Dombrock system) and DI*A/DI*B (Diego system) alleles and to evaluate the impact of genomic and self-declared ancestry on rare donor screening in admixed populations.Background: The antigens Hy and Di b demonstrate clinical importance. The lack of antisera for the serological evaluation of these antigens makes it necessary to develop molecular methods. In addition, considering that some rare red blood cell phenotypes present differences in frequency between ethnic groups, it is important to assess the applicability of self-declared ancestry in the search for rare donors in admixed populations.Methods: DO*HY and DI*A/DI*B genotyping based on real-time polymerase chain reaction (PCR) was standardised. A total of 457 blood donors clustered by selfdefined skin colour/race categories were genotyped. Furthermore, individual genomic ancestry was used in the analyses. Results:The assays developed are reproducible and provide satisfactory results even at low concentrations of DNA, which make them useful in situations where the DNA is scarce, such as dried blood spots on filter paper, or when screening for pooled samples. No significant difference was observed in the frequencies of the DI*A, DI*B and DO*HY, comparing the self-declared White (branco) donors with those who are Black (preto) and Brown (pardo). Conclusion:Real-time PCR, especially using pooled samples, is a promising strategy to screen rare blood donors. Although both self-reported race/colour and some blood group phenotypes are associated with ancestry, the results point to a greater complexity in the application of self-declared race/colour in the screening of rare donors in admixed populations. K E Y W O R D S ancestry, Diego, Dombrock, rare blood donor Amanda A. Muniz and Adão R. da Silva contributed equally to this study.

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Section: Discussionmentioning

confidence: 99%

The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self‐reported ancestry

Muniz

Silva

Ferraz

et al. 2019

Transfusion Medicine

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“…The implementation of RBC mass scale genotyping for donor screening has started in blood centers, especially in large collecting facilities [50][51][52][53]. The successful establishment of a blood group genotype database has already been accomplished aiming to fulfill antigen-negative requests, especially for SCD patients receiving regular transfusions, and to create an inventory of frozen red cell units with rare blood types [51].…”

Section: Rare Type Blood Donor Selectionmentioning

confidence: 99%

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

Sippert¹,

Volkova²,

Rios³

2021

Human Blood Group Systems and Haemoglobinopathies

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Blood transfusion is an effective therapeutic approach for several hematological conditions including sickle cell disease (SCD), thalassaemia, myelodysplastic syndrome (MDS), and autoimmune hemolytic anemia. It is also often indicated for transplantation and for patients receiving medical treatments for cancer. However, transfusion treatment can lead to the red blood cell (RBC) alloimmunization when an incompatible antigen is inadvertently present in the transfused blood. Alloantibodies can cause RBC destruction and many other complications defeating the purpose of the treatment. The risk of development of multiple alloantibodies increases with the frequency of transfusions in transfusion-dependent patients and can be mitigated by transfusing blood type negative for multiple antigens to prevent hemolysis. This chapter discusses the transfusion's risk of RBC alloimmunization as an adverse event; consequences of alloimmunization in patients' care; approaches to prevent and/or mitigate alloimmunization and enhance transfusion efficacy; application of RBC genotyping to supplement serology for preventing alloimmunization. The currently available techniques for RBC genotyping and the importance of reference reagents for determining the genotyping accuracy will also be discussed.Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization DOI: http://dx.doi.org/10.5772/intechopen.90095 Blood group system WHO IRR [98] CBER RR [89]Rh RHD positive (zygosity not determined)

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“…78,79 This facilitates access to compatible donors for alloimmunised patients. 81 Patients with antibodies to high-frequency antigens and antibodies to numerous antigens will benefit when tested with the NGS approach which provides the recipient's profile of antigens in one step. Moreover, if the donor is genotype-matched further alloantibody formation is limited.…”

Section: Applications Of Ngs In Transfusion Medicinementioning

confidence: 99%

<p>Potential of next-generation sequencing to match blood group antigens for transfusion</p>

Orzińska¹,

Guz²,

Brojer³

2019

IJCTM

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A review of the advances in applying next-generation sequencing (NGS) to transfusion medicine for the purpose of genotyping alleles encoding clinically important red blood cell and platelet antigens. NGS data from published studies confirm the possibility of antigen prediction based on sequencing of the whole genome, exome or targeted regions. What remains a challenge, to provide highly accurate NGS genotyping, is the further improvement of bioinformatic solutions for automated interpretation based on publicly accessible and improved reference databases appropriate for NGS methods as well as validation of a method based on the examination of a large number of individuals. There is no doubt, however, as to the future of NGS as a supplementary test used to provide highly compatible blood as well as to reduce the risk of patient's alloimmunization. This is part of personalized medicine.

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Towards a Regional Registry of Extended Typed Blood Donors: Molecular Typing for Blood Group, Platelet and Granulocyte Antigens

Cited by 18 publications

References 35 publications

The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self‐reported ancestry

The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self‐reported ancestry

Accuracy of Blood Group Typing in the Management and Prevention of Alloimmunization

<p>Potential of next-generation sequencing to match blood group antigens for transfusion</p>

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