Towards a super H2 producer: Improvements in photofermentative biohydrogen production by genetic manipulations

Kars, Gökhan; Gündüz, Ufuk

doi:10.1016/j.ijhydene.2010.04.037

Cited by 79 publications

(40 citation statements)

References 101 publications

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“…The variations in reported data from literatures confirm the complexity and flexibility of PNSB metabolism and therefore require investigation of each type of strain (Koku et al, 2002;Kim et al, 2012a). Kars and Gunduz, 2010) In our study, the use of acetate was associated with low H 2 production (Fig. 1), high cell density increase and high final pH (Table 3).…”

Section: The H 2 Production Of R Sphaeroides Kctc 1434 From Individusupporting

confidence: 61%

“…First, it requires Adenosine Triphosphate (ATP) and reducing power in the form of NADPH 2 which diminishes energy and electron output; and second, it shares common elements with Polyhydroxybutyrate (PHB) biosynthetic route (Kars and Gunduz, 2010). The production of polyhydroxyalkanoates (e.g., PHB) as an intracellular storage material is another route that competes with the H 2 production in disposing excess reducing equivalents in cells (Hustede et al, 1993).…”

Section: The H 2 Production Of R Sphaeroides Kctc 1434 From Individumentioning

confidence: 99%

“…The radical increase in pH could be explained by the nitrogenase-mediated H 2 production via the reaction (Liang and Burris, 1988;Kars and Gunduz, 2010 …”

Section: The Effect Of Headspace Gas On H 2 Productionmentioning

confidence: 99%

“…Among the different ways of producing H 2 from biomass, the use of Purple Non-Sulfur Bacteria (PNSB) such as Rhodobacter sphaeroides has been widely known due to their metabolic versatility, wide range of substrate utilization (Das and Veziroglu, 2001) and high product purity (Kars and Gunduz, 2010;Wu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the Photoheterotrophic Hydrogen Production of <i>Rhodobacter sphaeroides</i> KCTC 1434 using Volatile Fatty Acids under Argon and Nitrogen Headspace

Ventura¹,

Ventura²,

Oh³

2016

American Journal of Environmental Sciences

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Abstract:In this study, the photo fermentative H 2 production of Rhodobacter sphaeroides KCTC 1434 was investigated using acetate, propionate and butyrate under argon and nitrogen headspace gases. The highest H 2 yield and Substrate Conversion Efficiency (SCE) were observed from butyrate (8.84 mol H 2 /mol butyrate consumed, 88.42% SCE) and propionate (6.10 mol H 2 /mol propionate consumed, 87.16% SCE) under Ar headspace. Utilization of acetate was associated with low H 2 evolution, high biomass yield and high final pH, which suggest that acetate uptake by the strain involves a biosynthetic pathway that competes with H 2 production. The use of N 2 in sparging resulted to a decreased H 2 productivity in propionate (0.49 mol H 2 /mol propionate consumed, 7.01% SCE) and butyrate (1.22 mol H 2 /mol butyrate consumed, 1.04% SCE) and was accompanied with high biomass yield and radical pH increase in all acids. High H 2 generation had shown to improve acid consumption rate. The use of the three acids in a mixed substrate resulted to a drastic pH rise and lower H 2 generation. This suggests that a more refined culture condition such as additional control of pH during fermentation must be kept to enhance the H 2 productivity. Overall, the study provided a background on the H 2 production using R. sphaeroides KCTC 1434 which might be a good co-culture candidate because of its high SCE on butyrate and propionate.

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Section: The H 2 Production Of R Sphaeroides Kctc 1434 From Individusupporting

confidence: 61%

Section: The H 2 Production Of R Sphaeroides Kctc 1434 From Individumentioning

confidence: 99%

“…The radical increase in pH could be explained by the nitrogenase-mediated H 2 production via the reaction (Liang and Burris, 1988;Kars and Gunduz, 2010 …”

Section: The Effect Of Headspace Gas On H 2 Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of the Photoheterotrophic Hydrogen Production of <i>Rhodobacter sphaeroides</i> KCTC 1434 using Volatile Fatty Acids under Argon and Nitrogen Headspace

Ventura¹,

Ventura²,

Oh³

2016

American Journal of Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…Recently Redwood et al [6] and Kars and Gunduz [11] reviewed the use of mutants insensitive to NH þ 4 , indicating that genetic manipulation is a potential route to overcome nitrogenase inhibition by fixed-nitrogen-containing compounds including NH þ 4 . A number of strains bringing mutations in nifA have been obtained [8,12], all bringing single point mutations.…”

Section: Introductionmentioning

confidence: 99%