Towards an integrated linkage map of common bean 2. Development of an RFLP-based linkage map

Abstract

A restriction fragment length polymorphism (RFLP)-based linkage map for common bean (Phaseolus vulgaris L.) covering 827 centiMorgans (cM) was developed based on a F2 mapping population derived from a cross between BAT93 and Jalo EEP558. The parental genotypes were chosen because they exhibited differences in evolutionary origin, allozymes, phaseolin type, and for several agronomic traits. The segregation of 152 markers was analyzed, including 115 RFLP loci, 7 isozyme loci, 8 random amplified polymorphic DNA (RAPD) marker loci, and 19 loci corresponding to 15 clones of known genes, 1 virus resistance gene, 1 flower color gene, and 1 seed color pattern gene. Using MAPMAKER and LINKAGE-1, we were able to assign 143 markers to 15 linkage groups, whereas 9 markers remained unassigned. The average interval between markers was 6.5 cM; only one interval was larger than 30 cM. A small fraction (9%) of the markers deviated significantly from the expected Mendelian ratios (1∶2∶1 or 3∶1) and mapped into four clusters. Probes of known genes belonged to three categories: seed proteins, pathogen response genes, and Rhizobium response genes. Within each category, sequences homologous to the various probes were unlinked. The I gene for bean common mosaic virus resistance is the first disease resistance gene to be located on the common bean genetic linkage map.

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

“…Therefore, with a few exceptions, the different maps included few shared markers. The exceptions included the use of shared RFLP markers between the map developed at the University of California, Davis (Nodari et al, 1993a) and the map developed at the University of Paris XI (Adam-Blondon et al, 1994), along with a few isozyme and seed protein markers also shared with the map developed at the University of Florida Gepts et al (1993), Nodari et al (1993a) and Freyre et al (1998) BAT93  Jalo EEP558 (RI) a BJ Midas  G12873 (RI) MG Domestication syndrome: Ppd, fin, St, y, P; phenology, number of nodes and pods, seed weight; dormancy Koinange et al (1996) XR235-1-1  DIACOL Calima (BC) XD Resistance to: X. axonopodis Vallejos et al (1992) and Yu et al (1998) ( Vallejos et al, 1992). The total number of the shared markers, however, remained quite small.…”

“…For each of the LGs, a minimum of two (or more) markers from the different maps were mapped in the BJ population. The maps involved included the Davis map (Nodari et al, 1993a;Freyre et al, 1998), the Paris map (Adam-Blondon et al, 1994), the Florida map (Vallejos et al, 1992), and the Nebraska-Wisconsin maps (Jung et al, 1996a(Jung et al, , 1997. The former three maps are based on RFLP markers, wholly or in their majority.…”

“…Recent investigations have clarified the genetic control of the different colors and color patterns in bean seeds (Beninger et al, 2000). The P gene, which is the basic gene determining the presence (colored) or absence (white) of pigments in seeds (and elsewhere in the plant), and the asp gene, responsible for the dullness of the seedcoat, are located on LG B7 (Arndt and Gepts, 1989;Koinange et al, 1996), whereas the color modifying B gene is tightly linked to the I gene for bean common mosaic virus (BCMV) resistance on B2 (Temple and Morales, 1986;Nodari et al, 1993a). Other seedcoat color genes have also been mapped onto the core linkage map (McClean et al, 2002).…”

“…To the left of each LG, are the framework molecular markers (smaller font) and the biochemical genes (larger font) and major phenotypic trait genes (shaded boxes). For explanations on marker and biochemical gene symbols (Bassett, 1996; Tables 4 and 5 in Gepts, 1999), and the original map references (Vallejos et al, 1992;Nodari et al, 1993a;AdamBlondon et al, 1994). The Co and R VI loci are anthracnose resistance loci (Geffroy, 1997;Geffroy et al, 1999Geffroy et al, , 2000Kelly and Young, 1996;Young et al, 1998;Vallejo and Kelly, 2002).…”

Caracterização de linhagens endogâmicas recombinantes e mapeamento de locos de características quantitativas associados a ciclo e produtividade do feijoeiro-comum

“…Na última década, alguns marcadores isoenzimáticos têm sido usados para o mapeamento (Vallejos & Chase, 1991a, 1991b. O avanço das técnicas de biologia molecular e o desenvolvimento dos marcadores moleculares do DNA têm permitido a construção de mapas genéticos mais saturados para o feijoeiro-comum (Vallejos et al, 1992;Nodari et al, 1993;Miklas et al, 1998).…”