Towards an optimized MTT assay

Nikš, M; Otto, Matthias

doi:10.1016/0022-1759(90)90309-j

Cited by 218 publications

(98 citation statements)

References 7 publications

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“…Recently there have been numerous papers describing alternative, non-radioisotopic methods for measuring lymphocyte mitogenesis and cell mediated cytotoxicity for mammalian cells. One colorimetric method that has been generally accepted is to measure 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) reduction by viable cells, first proposed by Mosmann (1983) and subsequently modified (Tada et al, 1986;Hansen et al, 1989;Niks & Otto, 1990;Van de Loosdrecht et al, 1991). In this paper we describe the use of MTT for measuring in vitro lymphocyte mitogenesis in brook trout (Salvelinus fontinalis).…”

Section: Introductionmentioning

confidence: 99%

A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis

Daly

Moore

Olivier

1995

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 99%

A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis

Daly

Moore

Olivier

1995

Fish & Shellfish Immunology

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“…32,33 Exponentially growing cells (650 -1200/well) were plated in 50 L of media in 96-well microtiter plates. The outer wells of the plates were not used and were filled with 175 L of phosphate-buffered saline to provide added humidity.…”

Section: -(45-dimethylthiazol-2-yl)-25-diphenyltetrazolium Bromidementioning

confidence: 99%

Coexpression of rat glutathione S-transferase A3 and human cytidine deaminase by a bicistronic retroviral vector confers in vitro resistance to nitrogen mustards and cytosine arabinoside in murine fibroblasts

et al. 2000

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The transfer of drug resistance genes into hematopoietic cells is an experimental approach to protect patients from drug-induced myelosuppression. Because anti-cancer drugs are often administered in combination to increase their clinical efficacy, vectors that express two drug resistance genes are being developed to broaden the spectrum of chemoprotection. We have constructed a bicistronic vector, MFG/GST-IRES-CD (MFG/GIC) coexpressing rat glutathione S-transferase (GST) A3 isoform (rGST Yc 1 ) and human cytidine deaminase (CD). Murine NIH 3T3 fibroblast cells transduced with this vector were evaluated for their resistance to nitrogen mustards and cytosine nucleoside analogs. GIC-transduced polyclonal cell populations (GIC cells) demonstrated marked increases in selenium-independent glutathione peroxidase (peroxidase) and CD activities, as well as increased resistance to melphalan (2.3-fold), chlorambucil (3.4-fold), and cytosine arabinoside (Ara-C) (8.1-fold). After selection with Ara-C, the peroxidase and CD activities of GIC cells were augmented 2.6-and 2.9-fold, respectively, in comparison with unselected cells, and the resistance to melphalan, chlorambucil, and Ara-C was further increased to 3.7-, 5.9-, and 53-fold, respectively. Melphalan selection of GIC cells likewise augmented their peroxidase (2.3-fold) and CD (1.9-fold) activities. GIC cells proliferated in the simultaneous presence of melphalan and Ara-C at drug concentrations that completely inhibited the growth of untransduced cells. The growth rate of unselected GIC cells exposed to the drug combination averaged 18% that of drug-free cultures. The growth rate of GIC cells exposed to the drug combination increased to 30% of controls after Ara-C selection and to 50% after melphalan selection. Our results suggest that retroviral transfer of MFG/GIC may be useful for chemoprotection against the toxicities of nitrogen mustards and cytosine nucleoside analogs. Cancer Gene Therapy (2000) 7, 757-765

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“…Cytotoxicity assay Sensitivity of various cell lines to cis-DDP in vitro was determined by the MTT tetrazolium colorimetric assay. 39) Cells (1×10 4 cells per well) were plated in a 96-well plate, incubated overnight, and treated with cis-DDP at various concentrations up to 100 µM. The cells were incubated for 24 h, and each well was supplemented with 10 µl of 5 mg/ml MTT.…”

Section: Methodsmentioning

confidence: 99%

HeLa Cell Transformants Overproducing Mouse Metallothionein Show in vivo Resistance to cis‐Platinum in Nude Mice

Toyoda

Mizushima

Satoh

et al. 2000

Japanese Journal of Cancer Research

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Towards an optimized MTT assay

Cited by 218 publications

References 7 publications

A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis

A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte mitogenesis

Coexpression of rat glutathione S-transferase A3 and human cytidine deaminase by a bicistronic retroviral vector confers in vitro resistance to nitrogen mustards and cytosine arabinoside in murine fibroblasts

HeLa Cell Transformants Overproducing Mouse Metallothionein Show in vivo Resistance to cis‐Platinum in Nude Mice

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