2022
DOI: 10.1080/21623945.2022.2104514
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Towards better models for studying human adipocytesin vitro

Abstract: With obesity and its comorbidities continuing to rise, we urgently need to improve our understanding of what mechanisms trigger the white adipose tissue to become dysfunctional in response to over-feeding. The recent invent of 3D culturing models has produced several noteworthy protocols for differentiating unilocular adipocytes in vitro , promising to revolutionize the obesity research field by providing more representative adipose tissue models for such mechanistic studies. In parallel… Show more

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Cited by 9 publications
(2 citation statements)
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“…In the past 10 years, several new models of adipose tissue have been developed with varying degrees of complexity [ 11 ]. Many of these models support the 3D environment through the use of scaffolds, such as silk [ 12 , 13 ], Matrigel [ 14 ] or Geltrex [ 15 ], collagen [ 16 ], and fibrinogen [ 17 ]. More recently, efforts have been made to increase the complexity of adipose models by including other cell lineages that are found in native adipose tissue.…”
Section: Introductionmentioning
confidence: 99%
“…In the past 10 years, several new models of adipose tissue have been developed with varying degrees of complexity [ 11 ]. Many of these models support the 3D environment through the use of scaffolds, such as silk [ 12 , 13 ], Matrigel [ 14 ] or Geltrex [ 15 ], collagen [ 16 ], and fibrinogen [ 17 ]. More recently, efforts have been made to increase the complexity of adipose models by including other cell lineages that are found in native adipose tissue.…”
Section: Introductionmentioning
confidence: 99%
“…In vitro studies of lipedema adipose derived stem cells and preadipocytes aremainly performed in 2D cultures and in vitro differentiation of ASCs upon adipogenic stimulation is usually followed after 21–28 days of cultivation. Interestingly, in vitro differentiation, particularly in 2D cultures, leads to the accumulation of small multilocular lipid droplets, while freshly isolated mature adipocytes contain a single large unilocular LD [ 81 ] ( Figure 2 a–f).…”
Section: Introductionmentioning
confidence: 99%