Towards centralized biogas plants: Co-digestion of sewage sludge and pig manure maintains process performance and active microbiome diversity

Lavergne, Céline; Bovio-Winkler, Patricia; Etchebehere, Claudia; García-Gen, Santiago

doi:10.1016/j.biortech.2019.122442

Cited by 27 publications

(9 citation statements)

References 42 publications

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“…The Proteobacteria, the Bacteroidetes, the Firmicutes, the Actinobacteria, the Synergistetes, the Euryarchaeota, and Cloacimonetes were the dominant phyla in WWTP Marrakech. These data are similar to those previously obtained using marker gene-based analysis [39,40,52,53,55,[57][58][59]. At the OTU level (Figure 3), OTU 1 (Firmicutes), OTU 2 (Cloacimonetes), and OTU 3 (Bacteroidetes) were the most abundant of the four digesters.…”

Section: Discussionsupporting

confidence: 89%

Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Houari

Ranchou-Peyruse

et al. 2020

Processes

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Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1–5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 105 to 1.47 × 107 gene copies.g−1 of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 105 to 1.92 × 107 gene copies.g−1 of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.

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Section: Discussionsupporting

confidence: 89%

Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Houari

Ranchou-Peyruse

et al. 2020

Processes

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“…+ concentrations of the influent sewage [32]. The ammonium concentration was much lower in the case of anaerobic sludge, with a value of 279 mg/L.…”

Section: Chemical Characterizationmentioning

confidence: 92%

Nitrogen Removal by an Anaerobic Iron-Dependent Ammonium Oxidation (Feammox) Enrichment: Potential for Wastewater Treatment

Rodríguez

Cisternas

Serrano

et al. 2021

Water

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Nitrogen pollution in water is a growing concern. Anthropogenic activities have increased the amount of nitrogen released into watercourses, which harms human health and the environment, and causes serious problems, such as eutrophication. Feammox is a recently discovered biological pathway associated with the nitrogen cycle that has gained scientific interest. This process couples anaerobic ammonium oxidation with iron reduction. This work presents a study on the Feammox mechanism from the enrichment of an activated sludge obtained from a sewage treatment plant. The enrichment was carried out at neutral pH to study the N2 pathway, that is, the Feammox process with the oxidation of ammonium (NH4+) directly to N2. In addition, different sources of iron were studied: iron chloride (FeCl3); ferrihydrite; and goethite. The characterization of the sludge showed the genes associated with ammonia monooxygenase, nitrate and nitrite reductases processes, along with relevant microbial species. The enrichment, carried out for 42 days and monitored every 14 days, showed that FeCl3 as a source of Fe was more effective for the coupled process of oxidation of NH4+ and the reduction of Fe(III) to Fe(II). At the end of the enrichment period, a removal of 31% and 32.2% of NH4+, and an increase in Fe(II) concentration by 52.4 and 63.9 times regarding the initial value were achieved in aerobic and anaerobic sludge, respectively. This study provides information on the potential of Feammox in the removal of N from wastewater, and the oxidation/reduction yields in the initial enrichment phase.

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“…Paired‐end reads from the original DNA fragments were merged using FLASH (Version 1.2.7) (Magoč & Salzberg, 2011) and were assigned to each sample according to the unique barcodes. Sequences were analysed using QIIME 2 (v2022.2) software package (Lavergne et al., 2020). Chimeric sequences were removed using the USEARCH software based on the UCHIME algorithm (Edgar et al., 2011).…”

Section: Methodsmentioning

confidence: 99%

Gut bacterial community of Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is driven by rice varieties

Lin,

Zhang,

et al. 2024

J Applied Entomology

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To understand the effect of diet on gut bacteria of Cnaphalocrocis medinalis (rice leaf folder, RLF), we compared the composition and diversity of gut bacterial community in C. medinalis larvae collected from three different rice variety fields of Minghui 82 (RLF1), Youngyong 15 (RLF2) and Minghui 2155 (RLF3), using culture‐independent PCR (V3 variable region)‐denaturing gradient gel electrophoresis (PCR‐DGGE) methods and hypervariable region (V4) of 16S rRNA sequenced by Illumina HiSeq platform. The DGGE result showed that the bacteria genera Asaia, Bacillus, Stenotrophomonas, Achromobacter and Serratia coexisted in the guts of RLF1, RLF2 and RLF3, while Cedecea, Burkholderia and Acinetobacter coexisted in the guts of RLF2 and RLF3. However, only the genus Enterococcus existed in the guts of RLF1, and Pantoea, Wolbachia and Tumebacillus in RLF3 larvae. A total of 25 bacterial phyla, 48 classes, 127 orders, 223 families, and 406 genera were identified when using 16s RNA sequencing, with 35 genera coexisted in RLF1, RLF2 and RLF3 larval guts, while 83, 36, 141 unique genera existed in RLF1, RLF2 and RLF3, respectively. The indices of ACE and Shannon were not significantly different among RLF1, RLF2 and RLF3. The dominant bacterial taxa were Proteobacteria, Bacteroidetes and Firmicutes at the phylum level, and Acinetobacter and Wolbachia at the genus level. The relative genus abundance for the genera with relative abundance (≥0.01) was significantly different among RLF1, RLF2 and RLF3. The findings indicated that different rice varieties had significant effects on the relative abundance of gut bacteria in RLF, which could provide new insights into the relationship between insect gut bacteria and their associated host plants.

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Towards centralized biogas plants: Co-digestion of sewage sludge and pig manure maintains process performance and active microbiome diversity

Cited by 27 publications

References 42 publications

Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Nitrogen Removal by an Anaerobic Iron-Dependent Ammonium Oxidation (Feammox) Enrichment: Potential for Wastewater Treatment

Gut bacterial community of Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is driven by rice varieties

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