Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway

Analysis of creatine kinase activity with evaluation of protein expression under the effect of heat and hydrogen peroxide

“…This difference may arise due to the separate inactivation mechanisms. The parameters summarized in Table were consistent with those of CK from other studies [25]. CK possesses four cysteine residues and only one of them is believed to be essential for catalytic activity, where the other three are thought to be involved in CK folding [25][26][27].…”

“…The parameters summarized in Table were consistent with those of CK from other studies [25]. CK possesses four cysteine residues and only one of them is believed to be essential for catalytic activity, where the other three are thought to be involved in CK folding [25][26][27]. According to [2] muscle isoform of CK could retain its activity at temperatures below 50 °C and being completely in-activated after heat treatment at temperatures above 62 °C [28,29].…”