Towards dynamic coating of glass microchip chambers for amplifying DNAvia the polymerase chain reaction

Giordano, Braden C.; Copeland, Ebony R.; Landers, James P.

doi:10.1002/1522-2683(200101)22:2<334::aid-elps334>3.0.co;2-o

Cited by 100 publications

(61 citation statements)

References 26 publications

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“…On the other hand, passivation of the Si surface by silanization followed by a polymer coating showed good though inconsistent amplification, while SiO 2 performed best of all [214,215]. Alternatives to silanization for passivation of surfaces in glass PCR microchambers have also been explored by Giordano et al [216]. A distinctively different approach to on-chip PCR was introduced by Kopp et al [217].…”

Section: Clinical Dna By Microchip Pcrmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

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This review gives an overview of developments in the field of microchip analysis for clinical diagnostic and forensic applications. The approach chosen to review the literature is different from that in most microchip reviews to date, in that the information is presented in terms of analytes tested rather than microchip method. Analyte categories for which examples are presented include (i) drugs (quality control, seizures) and explosives residues, (ii) drugs and endogenous small molecules and ions in biofluids, (iii) proteins and peptides, and (iv) analysis of nucleic acids and oligonucleotides. Few cases of microchip analysis of physiological samples or other "real-world" matrices were found. However, many of the examples presented have potential application for these samples, especially with ongoing parallel developments involving integration of sample pretreatment onto chips and the use of fluid propulsion mechanisms other than electrokinetic pumping.

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Section: Clinical Dna By Microchip Pcrmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

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“…favorable fluidic, optical and electrical insulating properties), high resistance to fluid and vapor diffusion during PCR, and hence used in most integrated PCR-CE devices (Hong et al 2001;Khandurina et al 2000;Lagally et al 2004;Prakash et al 2005;Rodriguez et al 2003;Waters et al 1998). The main drawback in using glass is its hydrophilicity which causes excessive adsorption of PCR reagents and requires careful tailoring and/or suitable modification of its surface properties (Erill et al 2003;Giordano et al 2001a;Obeid et al 2003).…”

Section: Introductionmentioning

confidence: 99%

An integrated genetic analysis microfluidic platform with valves and a PCR chip reusability method to avoid contamination

Prakash

Kaler

2006

Microfluid Nanofluid

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Clinical diagnostics and genomic research often require performing numerous genetic tests. While microfluidic devices provide a low-cost alternative to such demands, integrated microfluidic devices are fabricated using expensive technology not always affordable for single use. However, carryover crosscontamination (CXC) concerns (i.e. either false positive or false negative PCR data) in PCR chips prevent reuse, defying much of the advantages of miniaturized systems developed using expensive MEMS processing. In this work, we present an integrated and reusable PCR-CE glass microfluidic chip capable of multichamber PCR and sequential CE, with emphasis on a unique chip reusability approach to avoid CXC. For reliable PCR, the surface of the chamber is re-configured from its virgin hydrophilic (CA < 20°) to hydrophobic (CA > 110°) by silanization. To then extend this silanization method as a chip reusability technique, the silanization coating is 'stripped and re-silanized' (SRS) to create a fresh coating prior to each successive PCR run. Experimental confirmation of the effectiveness of SRS method in avoiding the CXC is demonstrated using plasmid DNA and HIV-1 infected DNA samples. We also present passive plug microvalves incorporated in the chip to enable fluid/vapor retention during the PCR and controlled fluid flow from the PCR chamber to the CE section for further analysis.

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“…Different strategies for dynamic coating of glass microchip chambers for amplifying DNA via PCR have been investigated by the group of Landers [53]. While HEC was found to be suitable to deactivate microchannel surfaces to enable reliable DNA separations, it was observed that HEC at a concentration of 0.2% w/v significantly inhibited a-DNA amplification in polypropylene inserts.…”

Section: Coating Of Integrated Reactor Chambersmentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

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Different approaches and techniques for surface modification of microfluidic devices applied for microchip electrophoresis are reviewed. The main focus is on the improved electrophoretic separation by reducing analyte-wall interactions and manipulation of electroosmosis. Approaches and methods for permanent and dynamic surface modification of microfluidic devices, manufactured from glass, quartz and also different polymeric substrates, are described.

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Towards dynamic coating of glass microchip chambers for amplifying DNAvia the polymerase chain reaction

Cited by 100 publications

References 26 publications

Microfluidic chips for clinical and forensic analysis

Microfluidic chips for clinical and forensic analysis

An integrated genetic analysis microfluidic platform with valves and a PCR chip reusability method to avoid contamination

Surface modification in microchip electrophoresis

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