Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria

Alexandri, Maria; Hübner, Dennis; Schneider, Roland; Fröhling, Antje; Venus, Joachim

doi:10.1016/j.nbt.2022.08.003

Cited by 11 publications

(4 citation statements)

References 41 publications

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“…Using this procedure, most sugars were consumed by the mixed culture, reaching a LA concentration of 60 g/L with a yield of 0.55 g LA /g SBP with the strain Lactobacillus plantarum HII in the first step and Lactobacillus plantarum HII and Lactobacillus brevis PCM 488 in the second. The same strategy was followed by Alexandri et al after observing the non-complete consumption of the hydrolyzed sugars from SBP [96]. They produced 22 g/L of LA with a yield of 0.78 g LA /g SBP with the strains L. coryniformis subsp.…”

Section: Production Of La From Sbp Hydrolysatesmentioning

confidence: 94%

“…They produced 22 g/L of LA with a yield of 0.78 g LA /g SBP with the strains L. coryniformis subsp. torquens DSM 20005 and isolate A250 (most likely L. preudomesenteroides), adding the second strain when half of the glucose was consumed [96]. Additionally, a similar procedure was performed by Diaz et al, where SBP hydrolysate was fermented first to ethanol and then to LA with the strain Lactobacillus plantarum [73].…”

Section: Production Of La From Sbp Hydrolysatesmentioning

confidence: 99%

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Sugar Beet Pulp as Raw Material for the Production of Bioplastics

2023

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The production of bioplastics from renewable materials has gained interest in recent years, due to the large accumulation of non-degradable plastic produced in the environment. Here, sugar beet pulp (SBP) is evaluated as a potential raw material for the production of bioplastics such as polylactic acid (PLA) and polyhydroxyalkanoates (PHAs). SBP is a by-product obtained in the sugar industry after sugar extraction from sugar beet, and it is mainly used for animal feed. It has a varied composition consisting mainly of cellulose, hemicellulose and pectin. Thus, it has been used to produce different value-added products such as methane, hydrogen, pectin, simple sugars, ethanol, lactic acid and succinic acid. This review focuses on the different bioprocesses involved in the production of lactic acid and PHAs, both precursors of bioplastics, from sugars derived from SBP. The review, therefore, describes the pretreatments applied to SBP, the conditions most frequently used for the enzymatic hydrolysis of SBP as well as the fermentation processes to obtain LA and PHAs.

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Section: Production Of La From Sbp Hydrolysatesmentioning

confidence: 94%

Section: Production Of La From Sbp Hydrolysatesmentioning

confidence: 99%

Sugar Beet Pulp as Raw Material for the Production of Bioplastics

2023

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“…Lignocellulosic biomass is a natural material that can be used as a raw material for LA production. Lignocellulosic biomass can be obtained from sources like agricultural and industrial waste such as bagasse, grass, straw, corn cob, wood waste, molasses, sugar beet pulp, and coconut pulp (Ghaffar et al, 2014 ; Darwin et al, 2018 ; Alexandri et al, 2022 ). However, utilizing lignocellulosic biomass for LA production typically requires pretreatment to break down the complex structure of lignocellulose into fermentable sugars like glucose and sucrose.…”

Section: Lactic Acid Fermentation Feedstockmentioning

confidence: 99%

Biotechnological lactic acid production from low-cost renewable sources via anaerobic microbial processes

Kacaribu,

Darwin

2024

bta

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Lactic acid (LA) production from microbial fermentation using low-cost renewable sources has emerged as an attractive alternative to the use of petroleum-based products. This approach not only offers sustainable solutions for waste management but also enables the production of value-added products in an eco-friendly manner. However, to make this approach economically viable, optimizing the production process for high yield, productivity, and purity while minimizing costs is crucial. To address these challenges, various approaches have been proposed, including the use of neutralizing agents, high cell density cultures, co-cultures, fed-batch fermentation, and product removal strategies. Overall, this review underscores the potential of microbial fermentation for LA production as a sustainable and cost-effective solution to meet the growing demand for eco-friendly products. Further optimization of fermentation processes and the development of new microbial strains and fermentation techniques are key to advancing this approach. The production of LA through microbial fermentation presents a sustainable and eco-friendly solution to the increasing demand for eco-friendly products. With continued innovation, we can expect to see a significant reduction in the environmental impact of industrial processes, coupled with a more cost-effective and high-purity source of lactic acid for various industries.

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“…The current limitations and challenges for efficient microbial production of optically pure d -lactic acid are focusing on the development of cost-effective fermentation process with a high yield and high optical purity of lactic acid 3 . In the fermentation process, substrate for lactic acid production has been significantly considered as a key factor to enable the cost-effective fermentative production of optically pure lactic acid.…”

Section: Introductionmentioning

confidence: 99%

Association between organic nitrogen substrates and the optical purity of d-lactic acid during the fermentation by Sporolactobacillus terrae SBT-1

Thitiprasert,

Jaiaue,

Amornbunchai

et al. 2024

Sci Rep

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The development of biotechnological lactic acid production has attracted attention to the potential production of an optically pure isomer of lactic acid, although the relationship between fermentation and the biosynthesis of highly optically pure d-lactic acid remains poorly understood. Sporolactobacillus terrae SBT-1 is an excellent d-lactic acid producer that depends on cultivation conditions. Herein, three enzymes responsible for synthesizing optically pure d-lactic acid, including d-lactate dehydrogenase (D-LDH; encoded by ldhDs), l-lactate dehydrogenase (L-LDH; encoded by ldhLs), and lactate racemase (Lar; encoded by larA), were quantified under different organic nitrogen sources and concentration to study the relationship between fermentation conditions and synthesis pathway of optically pure lactic acid. Different organic nitrogen sources and concentrations significantly affected the quantity and quality of d-lactic acid produced by strain SBT-1 as well as the synthetic optically pure lactic acid pathway. Yeast extract is a preferred organic nitrogen source for achieving high catalytic efficiency of d-lactate dehydrogenase and increasing the transcription level of ldhA2, indicating that this enzyme plays a major role in d-lactic acid formation in S. terrae SBT-1. Furthermore, lactate racemization activity could be regulated by the presence of d-lactic acid. The results of this study suggest that specific nutrient requirements are necessary to achieve a stable and highly productive fermentation process for the d-lactic acid of an individual strain.

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Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria

Cited by 11 publications

References 41 publications

Sugar Beet Pulp as Raw Material for the Production of Bioplastics

Sugar Beet Pulp as Raw Material for the Production of Bioplastics

Biotechnological lactic acid production from low-cost renewable sources via anaerobic microbial processes

Association between organic nitrogen substrates and the optical purity of d-lactic acid during the fermentation by Sporolactobacillus terrae SBT-1

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