2017
DOI: 10.1002/jor.23793
|View full text |Cite
|
Sign up to set email alerts
|

Towards engineering distinct multi‐lamellated outer and inner annulus fibrosus tissues

Abstract: The annulus fibrosus (AF) of the intervertebral disc (IVD) has a zonal distribution of phenotypically distinct cells. The outer AF (OAF) cells produce an extracellular matrix (ECM) rich in type I collagen with little proteoglycans, whereas the ECM of the inner AF (IAF) has abundant type II collagen and proteoglycans. The inhomogeneous distribution of the ECM in the AF may reflect the complex mechanical forces that the IVD experiences. A bioengineered AF tissue should recapitulate both the inner and outer zones… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
10
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 16 publications
(10 citation statements)
references
References 50 publications
0
10
0
Order By: Relevance
“…However, the ECM composition of AF tissue changes between the outer region, which is abundant in collagen type I with little proteoglycans, and the inner region, which is rich in collagen type II and proteoglycans 88 . Supplementation of culture media with insulin transferrin‐selenium, proline, dexamethasone, and pyruvate to a multi‐lamellated outer and inner AF engineered tissue was able to promote the accumulation of collagen type I in the outer region and aggrecan and collagen type II the in inner region, replicating the ECM distribution of the native tissue 89 …”
Section: Electrospinningmentioning
confidence: 96%
See 1 more Smart Citation
“…However, the ECM composition of AF tissue changes between the outer region, which is abundant in collagen type I with little proteoglycans, and the inner region, which is rich in collagen type II and proteoglycans 88 . Supplementation of culture media with insulin transferrin‐selenium, proline, dexamethasone, and pyruvate to a multi‐lamellated outer and inner AF engineered tissue was able to promote the accumulation of collagen type I in the outer region and aggrecan and collagen type II the in inner region, replicating the ECM distribution of the native tissue 89 …”
Section: Electrospinningmentioning
confidence: 96%
“…88 Supplementation of culture media with insulin transferrin-selenium, proline, dexamethasone, and pyruvate to a multi-lamellated outer and inner AF engineered tissue was able to promote the accumulation of collagen type I in the outer region and aggrecan and collagen type II the in inner region, replicating the ECM distribution of the native tissue. 89 Considering the beneficial effects on ECM components in maintaining and/or directing cell phenotype, 90 and degradation rate. 91 Given the importance of mechanical loading in disc degeneration and regeneration, bioreactor systems have been utilized to provide more physiologically relevant conditions for the development of functional IVD substitutes.…”
Section: Optimization Of Cells and Cues For Ivd Tementioning
confidence: 99%
“…Inclusion of an endplate region to stimulate integration of tissue‐engineered discs in vitro and in vivo has also been attempted in constructs composed of layered electrospun polycarbonate urethane for the AF region, surrounding a calcium polyphosphate cylinder seeded directly with NP cells . These constructs were cultured for 2 weeks in serum‐containing media prior to implantation in a defect created in the bovine caudal disc space and adjacent vertebral body for 4 weeks .…”
Section: Progress In Whole Disc Tissue Engineeringmentioning
confidence: 99%
“…After Inclusion of an endplate region to stimulate integration of tissueengineered discs in vitro and in vivo has also been attempted in constructs composed of layered electrospun polycarbonate urethane for the AF region, surrounding a calcium polyphosphate cylinder seeded directly with NP cells. 53,66 These constructs were cultured for 2 weeks in serum-containing media prior to implantation in a defect created in the bovine caudal disc space and adjacent vertebral body for 4 weeks. 48 While there was histological evidence of integration between the engineered disc components after in vivo implantation, these constructs were not sized to replace the whole bovine caudal disc, and there was evidence of subsidence into the vertebral body.…”
Section: In Vivo Evaluation Of Engineered Discs In the Spinementioning
confidence: 99%
“…Several studies have combined two different biomaterials to create an engineered whole disc replacement. , One composite whole disc replacement, using PLA mesh for the AF and alginate gel for the NP, exhibited increased ECM over time in vitro , some integration between the two regions after 12 weeks in culture, an increase in mechanical properties over time, and shows promise in maintaining disc height in an animal model. , Another composite whole disc replacement using electrospun PCL for the AF and agarose for the NP observed compressive stress relaxation behavior and an increase in compressive modulus over weeks in culture. Additional animal studies of this implant highlighted the need for integration into the surrounding anatomy, so engineered end plates of an additional material were added to the design. , These types of complex tissue engineered whole disc approaches recapitulate anatomical and some mechanical properties of the disc and show promise for restoring disc height .…”
Section: Introductionmentioning
confidence: 99%