Towards high resolution maps of the mouse and human genomes—a facility for ordering markers to 0.1 cM resolution

Breen, Maria; Deakln, Lisa; Macdonald, B; Miller, Steve; Sibson, Ross; Tarttelln, Emma; Avner, Philip; Bourgade, Franck; Guénet, Jean-Louis; Montagutelli, Xavler; Polrier, Christophe; Simon, Dominique; Tailor, Dillp; Bishop, Mark; Kelly, M.; Rysavy, Francis; Rastan, Sohaila; Norris, Dominic P.; Shepherd, David; Abbott, Catherine M.; Pllz, Alison; Hodge, Sarah J.; Jackson, Ian J.; Boyd, Yvonne; Blair, Helen J.; Maslen, G.; Todd, John A.; Reed, Peter; Stoye, Jonathan P.; Ashworth, Alan; McCarthy, Linda; Cox, Roger; Schalkwyk, Leonard C.; Lehrach, Hans; Klose, Joachim; Gangadharan, Uma; Brown, Steve

doi:10.1093/hmg/3.4.621

Cited by 157 publications

(47 citation statements)

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“…Although no known insertion loci were found to be occupied in our previous studies of CD2-myc lympho- (Breen et al, 1994). Genetic distances (cM) are also indicated.…”

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confidence: 72%

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til-1: a novel proviral insertion locus for Moloney murine leukaemia virus in lymphomas of CD2-myc transgenic mice

Stewart

Terry

O'Hara

et al. 1996

Journal of General Virology

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Moloney murine leukaemia virus (MoMLV) markedly accelerates thymic lymphoma development in mice carrying a transgene in which the human c-myc gene is linked to the CD2 locus control region. To investigate the mechanism of synergy and identify the genes which collaborate with myc in these clonal tumours, we analysed the sites of MoMLV insertion. Analysis of known viral integration loci revealed only a small number of insertions at bmi-1, pim-1 and ahi-1. Further cloning and hybridization analysis revealed a new common integration locus, designated till, which was occupied in 25 out of 77 lymphomas examined, with evidence of multiple clonal insertions in some cases. Mapping relative to established chromosomal markers in interspecific backcross mice located till to mouse chromosome 17, distal to pim-1 and tic-1. These results suggest that the til-1 locus may harbour a novel myecollaborating gene which acts as a target for activation in T cell lymphomas.Proviral insertional mutagenesis is a pivotal event in oncogenesis by murine leukaemia virus (MLV) and the study of viral insertion sites in MLV-induced turnouts has led to the identification of a large number of host cell genes relevant to this process (Kung et al., 1991).Moreover, the discovery that transgenic mice carrying a dominant oncogene develop tumours much more rapidly if infected early in life with MLV has provided a powerful approach to the identification and complementation mapping of the host cell genes which drive the multi-step evolution of lymphomas (Berns, 1994). As an example, Eta-myc mice developed B cell lymphomas very rapidly after infection with Moloney MLV (MoMLV), with frequent insertions at several distinct host cell loci including pim-1, pal-1, bmi-1 and bla- 1 (van Lohuizen et al., 1991). The importance of these insertions to lymphoma development has been amply confirmed for pim-1 and bmi-1, both of which can act independently as dominant oncogenes and are strongly synergistic with myc in lymphomagenesis van Lohuizen et al., 1989;Haupt et al., 1993). Moreover, a reciprocal pattern of gene activation was seen in pim-1 transgenic mice which developed low incidence lymphomas that could be accelerated by neonatal infection with * Author for correspondence. Fax +44 141 330 6467. e-mail gpma03 @udcf.gla.ac.uk MoMLV, with concomitant insertions at c-myc, N-myc and paL1 loci (van Lohuizen et al., 1989).To test the effects of overexpressing c-myc in the T cell lineage, we developed a transgenic model in which the human c-myc oncogene was linked to a T cell-specific regulatory element, the locus control region from the human CD2 gene. We found that CD2-myc transgenic lines developed thymic lymphomas at low frequency and that neonatal infection with MoMLV dramatically accelerated this process, leading to 100% tumour incidence within 80 days of birth (Stewart et al., 1993).The tumours were of a homogeneous T cell phenotype (CD3 +, CD8 +, CD4 +/ ), invariably expressed the transgene and contained clonal integrations of MoMLV. Surprisingly, an initial ...

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“…Although no known insertion loci were found to be occupied in our previous studies of CD2-myc lympho- (Breen et al, 1994). Genetic distances (cM) are also indicated.…”

mentioning

confidence: 72%

“…Towards this goal, we established the chromosomal location of til-1 using the European Interspecific Backcross (Breen et al, 1994). An SstI polymorphism was established by probing parental DNAs (C57BL/6 and Mus spretus) with the til-lA probe and used to follow the segregation of the locus in 64 random backcross progeny.…”

mentioning

confidence: 99%

til-1: a novel proviral insertion locus for Moloney murine leukaemia virus in lymphomas of CD2-myc transgenic mice

Stewart

Terry

O'Hara

et al. 1996

Journal of General Virology

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“…F 1 females were mated to B6 males to produce an N 2 generation that became part of the EUCIB mapping panel (Breen et al 1994;Rhodes et al 1998). N 2 males (N ¼ 157)…”

Section: Methodsmentioning

confidence: 99%

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

Burgio

Szatanik

Guénet³

et al. 2007

Genetics

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Complex traits are under the genetic control of multiple genes, often with weak effects and strong epistatic interactions. We developed two new collections of mouse strains to improve genetic dissection of complex traits. They are derived from several backcrosses of the Mus spretus SEG/Pas or STF/Pas strains on the C57BL/6J background. Each of the 55 interspecific recombinant congenic strains (IRCSs) carries up to eight SEG/Pas chromosomal segments with an average size of 11.7 Mb, totalizing 1.37% of the genome. The complete series covers 39.7% of the SEG/Pas genome. As a complementary resource, six partial or complete interspecific consomic strains were developed and increased genome coverage to 45.6%. To evaluate the usefulness of these strains for QTL mapping, 16 IRCSs were compared with C57BL/6J for seven hematological parameters. Strain 66H, which carries three SEG/Pas chromosomal segments, had lower red blood cell volume and higher platelet count than C57BL/6J. Each chromosomal segment was isolated in a congenic strain to evaluate individual effects. Congenic strains were combined to assess epistasis. Our data show that both traits were controlled by several genes with complex epistatic interactions. IRCSs are therefore useful to unravel QTL with small effects and gene-by-gene interactions.

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“…Backcross progeny were initially typed for 78 primary anchor loci spanning the entire genome with 3-6 anchors per chromosome (Breen et al 1994). Subsequently, a number of additional anchor markers were added.…”

Section: Identification Of Panels Of Recombinants From Eucib For Highmentioning

confidence: 99%

“…Recently, we reported the construction of a high-resolution mouse mapping resource consisting of an interspecific backcross of nearly 1000 progeny (Breen et al 1994). A backcross of this size has a genetic resolution of 0.3 cM at the 95% confidence level that equates to ∼600 kb in the mouse genome.…”

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confidence: 99%

A High-Resolution Microsatellite Map of the Mouse Genome

Rhodes¹,

Straw²,

Fernando³

et al. 1998

Genome Res.

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The European Collaborative Interspecific Backcross (EUCIB) resource was constructed for the purposes of high-resolution genetic mapping of the mouse genome (Breen et al. 1994). The large Mus spretus/C57BL/6 backcross of 982 progeny has a genetic resolution of 0.3 cM at the 95% confidence level (∼500 kb in the mouse genome). We have used the EUCIB mapping resource to develop a genome-wide high-resolution genetic map incorporating 3368 microsatellites. The microsatellites are distributed among 2302 genetically separated bins with 1.46 markers per bin on average. Average bin separation is 0.61 cM. This high-resolution genetic map will aid the construction of a robust physical map of the mouse genome.

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Towards high resolution maps of the mouse and human genomes—a facility for ordering markers to 0.1 cM resolution

Cited by 157 publications

References 0 publications

til-1: a novel proviral insertion locus for Moloney murine leukaemia virus in lymphomas of CD2-myc transgenic mice

til-1: a novel proviral insertion locus for Moloney murine leukaemia virus in lymphomas of CD2-myc transgenic mice

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

A High-Resolution Microsatellite Map of the Mouse Genome

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