Towardsin vivointradermal surface enhanced Raman scattering (SERS) measurements: silver coated microneedle based SERS probe

Abstract

Journal of BIOPHOTONICSWe propose a microneedle coated with silver (Ag) to detect analytes at low concentrations positioned at a depth of more than 700 mm below the surface of a skin phantom with absorbers and scatterers for mimicking the intradermal surface-enhanced Raman scattering (SERS) measurements. The Ag layer in the Ag-coated microneedle-based probe is found to be the key to the effective detection of analytes buried inside the aforesaid phantom. Glucose concentrations ranging from 5 to 150 mM inside phantoms can be estimated with a root mean square error (RMSE) of 3.3 mM. This work shows the potential of using microneedles for simple in vivo intradermal SERS measurements of analytes with clinical relevance.SERS spectra of R6G molecules positioned inside the bottom layer of an agar phantom at a depth of 760 mm below the surface (inset) measured by using Ag-coated microneedle (conc: 10 -4 M; P EX : 5 mW), microneedle (conc: 10 -2 M; P EX : 5 mW), and ordinary Raman (conc: 10 -2 M; P EX : 100 mW). P EX means the excitation power and conc means concentration of R6G in the bottom layer.

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

“…HCPCF provides better light confinement and a larger interaction length for the guided light and the analyte, resulting in improved sensitivity to detect low concentrations in extremely low sample volumes. A microneedle was coated with silver to detect analytes at low concentrations positioned at a depth of more than 700 µm below the surface of a skin phantom with absorbers and scatterers for mimicking intradermal SERS measurements . Glucose concentrations ranging from 5 mM to 150 mM inside phantoms were estimated with a root mean square error of 3.3 mM.…”

Investigation of magnetic field enriched surface enhanced resonance Raman scattering performance using Fe3O4@Ag nanoparticles for malaria diagnosis

“…4 We employed the DDA model since the technique is more efficient and flexible (comparing to the multiple multipole method and the finite difference time domain method). 5 The Amsterdam DDA code 6 Figure 1 shows the SERRS spectra of β -hematin (a) without and (b) with the influence of an external magnetic field by using different Fe 3 O 4 @Ag nanoparticles. Prominent Raman peaks were observed at 754, 1120, 1570, and 1628 cm -1 , which correspond to ν 15 (D 4h notation system for resonance Raman peaks studies on myoglobin), 21 ν 22 , ν 2 , and ν 10 , respectively.…”

Engineering Microneedles for Therapy and Diagnosis: A Survey

“…MNs can pierce skin and its surface of the needles can be used as SERS substrate with simple modification. Yuen and Liu developed an Ag-coated MN probe based on intradermal SERS detection ( Figure 5B) [126]. A 750 nm thick Ag film was coated on MN surface as the Raman scattering enhanced substrate.…”