Towards instantaneous quantitative fluoroimaging drugs determination in body fluids with no added reagents

Abstract

Our objective is to develop a simple monitoring technique for rapid, sensitive and quantitative drugs detection in body fluids, with no reagent added and no need for qualified professionals. The user-friendly automatic Fluo-imager will (a) measure the full-range 3D fluorescence map of the inserted fluid sample, (b) determine the chemical nature and concentration of the drugs and (c) transfer the results through internet to the diagnosis center. For these goals the fluorescence measurement data will be examined by neuronal network-pattern recognition software. The software identifies the chemical nature and the appropriate concentration of the drug by comparison of the obtained 3D pattern with the contents of the data bank. One of the problems in the approach under consideration is the high optical density of body fluids in the UV region, which raises difficulties in the fluorescence measurements. In this paper, we have attempted to overcome this problem by means of preliminary dilution. Nevertheless, the problem of subtraction of the fluid fluorescence background still needs to be addressed.

Spectroscopic methods for detection of impurities in water

“…It should be emphasized that this method provides a general picture while the synchronous fluorescence is equivalent to the information included in any diagonal line along the 3-D contour map, which represents simultaneous scan of emission and excitation wavelengths with a constant wavelength separation. The fluorescence synchronous spectroscopy method has been applied first into qualitative and quantitative detection of various luminescent organic-aromatic compounds 3 and later has been applied towards trace analysis 4 , human serum 5 and body fluid drug-analysis 6 , water samples 7 , as well as into studying photochemical and photophysical mechanisms of excited aromatic compounds. In this context, our goal is to develop a compact, robust, deployable, and easy-to-use analytical tool for fast, accurate, and continuous analytical measurements.…”