Towards next-generation model microorganism chassis for biomanufacturing

Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses

“…The development of efficient green bio-manufacturing technology based on excellent microbial chassis cell performance is a circular economy transformation technology with great application potential [ 44 ]. Our objective in this study was engineered microbial chassis cells from the physiological perspective, and compared with previous studies, the engineering strategy in this study systematically analyzed the effects of chassis cell lifespan and morphology on industrial production.…”

Bacillus subtilis chassis in biomanufacturing 4.0

“… 1 Recent developments in smart biomanufacturing and Biological Design Automation usher BM 4.0 into a new era (Table 1). 2‐4 …”

“…1 Recent developments in smart biomanufacturing and Biological Design Automation usher BM 4.0 into a new era (Table 1). [2][3][4] The application of chassis in BM 4.0 and the emergence of enabling technologies would drive the translation of the SynBio chassis from laboratory to industrial applications by minimizing regulating complexity and enhancing biological entity performance predictability, although synthetic biologists originally expected to study chassis, also referred to as the 'living machine,' as a way to decipher the nature of life. 5 Bacillus subtilis, a robust, fieldable and well-characterized host is likely one of the most promising chassis for BM 4.0, 6,7 considering that B. subtilis can biosynthesize value-added products such as single-chain antibodies, nucleotides, vitamins, antibiotics, biosurfactants, carbohydrates, and biodegradable polymers [8][9][10][11][12][13] (Table 2).…”

Bacillus subtilis Chassis in Biomanufacturing 4.0

“…1) [1]. Recent developments in smart biomanufacturing and Biological Design Automation usher BM 4.0 into a new era (Table 1) [2][3][4].…”