Towards organ printing: engineering an intra-organ branched vascular tree

Visconti, Richard P.; Kasyanov, Vladimir; Gentile, Carmine; Zhang, Jing; Markwald, Roger R.; Mironov, Vladimir

doi:10.1517/14712590903563352

Cited by 210 publications

(148 citation statements)

References 100 publications

(108 reference statements)

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“…This technology has already been used to engineer constructs that mimic aspects of the anatomical and structural complexity of relatively thin tissues and hollow tubes such as skin [4] , blood vessels [5] and articular cartilage [6] . However reproducing the complex cellular and extra-cellular micro-organisation of an entire solid organ is well beyond the capabilities of currently available bioprinting technologies.…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Daly

Cunniffe

Sathy

et al. 2016

Adv Healthcare Materials

224

179

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The ability to print defined patterns of cells and extracellular-matrix components in three dimensions has enabled the engineering of simple biological tissues, however bioprinting functional solid organs is beyond the capabilities of current biofabrication technologies. An alternative approach would be to bioprint the developmental precursor to an adult organ, using this engineered rudiment as a template for subsequent organogenesis in vivo. Here we demonstrate that developmentally inspired hypertrophic cartilage templates can be engineered in vitro using stem cells within a supporting gamma-irradiated alginate bioink incorporating Arg-Gly-Asp (RGD) adhesion peptides.Furthermore, these soft tissue templates can be reinforced with a network of printed polycaprolactone fibres, resulting in a ~350 fold increase in construct compressive modulus providing the necessary stiffness to implant such immature cartilaginous rudiments into load bearing locations. As a proofof-principal, multiple-tool biofabrication was used to engineer a mechanically reinforced cartilaginous template mimicking the geometry of a vertebral body, which in vivo supported the development of a vascularized bone organ containing trabecular-like endochondral bone with a supporting marrow structure. Such developmental engineering approaches could be applied to the biofabrication of other solid organs by bioprinting pre-cursors that have the capacity to mature into their adult counterparts over time in vivo.3

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Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Daly

Cunniffe

Sathy

et al. 2016

Adv Healthcare Materials

224

179

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show abstract

“…The composition of biopapers, or a matrix upon which the ink is applied during printing, is another consideration in some applications. An interesting new development for applications employing human pluripotent stem cells is the announcement by GE Healthcare that a serum-derived protein supplement in a completely defined, xeno-free medium can support stable culture of human pluripotent stem cells on untreated matrix [25] .…”

Section: Application-specific Factorsmentioning

confidence: 99%

Digital biomanufacturing supporting vascularization in 3D bioprinting

Whitford¹,

Hoying²

2017

ijb

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Synergies in bioprinting are appearing from individual researchers focusing on divergent aspects of the technology. Many are now evolving from simple mono-dimensional operations to model-controlled multi-material, interpenetrating networks using multi-modal deposition techniques. Bioinks are being designed to address numerous critical process parameters. Both the cellular constructs and architectural design for the necessary vascular component in digitally biomanufactured tissue constructs are being addressed. Advances are occurring from the topology of the circuits to the source of the of the biological microvessel components. Instruments monitoring and control of these activates are becoming interconnected. More and higher quality data are being collected and analysis is becoming richer. Information management and model generation is now describing a "process network." This is promising; more efficient use of both locally and imported raw data supporting accelerated strategic as well as tactical decision making. This allows real time optimization of the immediate bioprinting bioprocess based on such high value criteria as instantaneous progress assessment and comparison to previous activities. Finally, operations up-and down-stream of the deposition are being included in a supervisory enterprise control.

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“…Limited material selection and lack of submicro-scale structural resolution are the major shortcomings of these techniques. Bioprinting (94), in which cells and matrix are deposited dropwise, has been developed over the past decade but also is a slow, serial process with limitations on print resolution, materials, and cells. In contrast to these methods, 3D sacrificial molding provides an intriguing alternative (95)(96)(97).…”

Section: Three-dimensional (3d) Printed Vesselsmentioning

confidence: 99%

Bioengineered Vascular Scaffolds: The State of the Art

et al. 2014

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To date, there is increasing clinical need for vascular substitutes due to accidents, malformations, and ischemic diseases. Over the years, many approaches have been developed to solve this problem, starting from autologous native vessels to artificial vascular grafts; unfortunately, none of these have provided the perfect vascular substitute. All have been burdened by various complications, including infection, thrombogenicity, calcification, foreign body reaction, lack of growth potential, late stenosis and occlusion from intimal hyperplasia, and pseudoaneurysm formation. In the last few years, vascular tissue engineering has emerged as one of the most promising approaches for producing mechanically competent vascular substitutes. Nanotechnologies have contributed their part, allowing extraordinarily biostable and biocompatible materials to be developed. Specifically, the use of electrospinning to manufacture conduits able to guarantee a stable flow of biological fluids and guide the formation of a new vessel has revolutionized the concept of the vascular substitute. The electrospinning technique allows extracellular matrix (ECM) to be mimicked with high fidelity, reproducing its porosity and complexity, and providing an environment suitable for cell growth. In the future, a better knowledge of ECM and the manufacture of new materials will allow us to “create” functional biological vessels - the base required to develop organ substitutes and eventually solve the problem of organ failure.

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Towards organ printing: engineering an intra-organ branched vascular tree

Cited by 210 publications

References 100 publications

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering

Digital biomanufacturing supporting vascularization in 3D bioprinting

Bioengineered Vascular Scaffolds: The State of the Art

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