Towards polarization sensitive tomographic diffraction microscopy: quantitative 3D differential interference contrast microscopy

Abstract

Tomographic diffraction microscopy is a 3D marker-less imaging technique allowing for quantitative characterization of the complex refractive index of the investigated sample. The method is a generalization of digital holography with a full control of the sample's illumination angle. Combining several holographic acquisitions through a 3D synthetic aperture process makes it possible to assess the optical field scattered by a 3D object. Recently we proposed to take benefits of the democratization of polarization array sensors to characterize birefringent samples. With this presentation, we demonstrate that using the same experimental configuration, with a modified demosaicking procedure, we can produce 3D Differential Interference Contrast microscope images of isotrope sample.