Towards real-time airborne pathogen sensing: Electrostatic capture and on-chip LAMP based detection of airborne viral pathogens

Jayakumar, Nitin; Caffrey, Veronique; Caffrey, Michael; Paprotny, Igor

doi:10.1016/j.snb.2024.135767

Sensors and Actuators B: Chemical

2024

DOI: 10.1016/j.snb.2024.135767

|View full text |Cite

Towards real-time airborne pathogen sensing: Electrostatic capture and on-chip LAMP based detection of airborne viral pathogens

Nitin Jayakumar,

Veronique Caffrey,

Michael Caffrey

et al.

Help me understand this report

View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

VLP-based model for the study of airborne viral pathogens

Caffrey,

Jayakumar,

Caffrey

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

The recent COVID-19 pandemic has underscored the danger of airborne viral pathogens. The lack of model systems to study airborne pathogens limits the understanding of airborne pathogen distribution as well as potential surveillance and mitigation strategies. In this work, we develop a novel model system to study airborne pathogens using virus-like particles (VLPs). Specifically, we demonstrate the ability to aerosolize VLP and detect and quantify aerosolized VLP RNA by reverse transcription-loop-mediated isothermal amplification in real-time fluorescent and colorimetric assays. Importantly, the VLP model presents many advantages for the study of airborne viral pathogens: (i) similarity in size and surface components; (ii) ease of generation and noninfectious nature enabling the study of biosafety level 3 and biosafety level 4 viruses; (iii) facile characterization of aerosolization parameters; (iv) ability to adapt the system to other viral envelope proteins, including those of newly discovered pathogens and mutant variants; and (v) the ability to introduce viral sequences to develop nucleic acid amplification assays. IMPORTANCE The study and detection of airborne pathogens are hampered by the lack of appropriate model systems. In this work, we demonstrate that noninfectious virus-like particles (VLPs) represent attractive models to study airborne viral pathogens. Specifically, VLPs are readily prepared, are similar in size and composition to infectious viruses, and are amenable to highly sensitive nucleic acid amplification techniques.

show abstract

VLP-based model for the study of airborne viral pathogens

Caffrey,

Jayakumar,

Caffrey

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Towards real-time airborne pathogen sensing: Electrostatic capture and on-chip LAMP based detection of airborne viral pathogens

Cited by 1 publication

References 48 publications

VLP-based model for the study of airborne viral pathogens

VLP-based model for the study of airborne viral pathogens

Contact Info

Product

Resources

About