Due to rapid changes in urban and rural economic development, the Chinese landscape has been gradually transforming toward urbanization. Most Chinese rural villages face declining problems such as population loss, land use transformation, fragmentation and abandonment, resulting in big changes in the rural spatial morphology. To understand these urbanization challenges, this study established a multi-factor methodology and applied it to a case study of three selected typical villages in southern Jiangsu Province. From this analysis, the quantification of the rural spatial morphology and environmental status, from 2005 to 2016, was determined. The eight driving factors established considered the rural geological location, landform, and social economic status. To analyze the driving factors, a quantitative analysis using ArcGIS, Environment for Visualizing Images (ENVI), and Analytic Network Process (ANP) decision-making methods were used. The results revealed mechanisms between the changes to spatial morphology of rural villages in southern Jiangsu Province and their key driving factors. This study provides data support and a theoretical framework to guide future development and policy of rural villages of different types, which supports the sustainable development of Chinese rural villages.