Software-defined networking (SDN) is a framework that enhances scalability and agility in network simplicity and control. It is characterized by logical centralization, improving control, and data planes. Nevertheless, additional investigation is needed to fully understand the influence of flow conflict on the transfer rate variable. The present research aims to analyze the impact of flow conflict on the efficacy of the two protocols, the transmission control protocol (TCP) and the user datagram protocol (UDP), respectively, by utilizing throughput as a measure of efficiency. The measurements are verified through SDN OpenFlow networking modeling using MININET. The findings reveal a significant average alteration in transfer rate for TCP and UDP when SDN conflict rules are present, ultimately impacting network and operational efficiency.