Towards standardization of canine STRs: a proposed nomenclature for six markers from the ISAG comparison‐test panel

Ciampolini, Roberta; Cecchi, Francesca; Bramante, Assunta; Tancredi, Mariella; Presciuttini, Silvano

doi:10.1111/j.1365-2052.2011.02267.x

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…The definitive breed standard was approved by ENCI in February 1949 and nine months later, in November, the Società Amatori Bracco Italiano (Association of Italian Bracco Amateurs, SABI) was founded. At the present the studies on the genetic characterization by microsatellites of the Bracco Italiano breed, using ISAG panel as suggested by the international ISAG canine comparison test (Ciampolini et al, 2012), showed a low genetic variability of the breed (Ciampolini et al, 2011).…”

Section: Introductionmentioning

confidence: 74%

Genetic Variability inBracco ItalianoDog Breed Assessed by Pedigree Data

Cecchi

Paci

Spaterna

et al. 2013

Italian Journal of Animal Science

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The Bracco Italiano is one of the oldest pointing dog breed, used for hunting ever since the Renaissance time. The complete electronic record of the breed was downloaded from the ENCI database [whole population (WP) = 24,613 animals registered since 1970 to 2011] with the aim to estimate genetic variability in Bracco Italiano dog breed using pedigree records. Up to 97% of the individuals had registered parents and 86% registered grandfathers. Average generation interval was 4.68±0.545 for stallions and 4.08±0.321 year for dams. Reference population (RP) was defined as the population of interest that include living reproductive animals approaching the last three generations and include 9006 dogs of which 34% were inbreds. The number of ancestors was 564 in WP and 188 in RP, while the effective number of ancestors was 46 and 34 respectively. To explain 50% of the genetic variability, a total of 18 and 9 ancestors enough, respectively in the WP and RP. The average inbreeding coefficient in the RP resulted 6.7% while the average increase in inbreeding was estimated to be 1.29% (Ne=38.86). Nevertheless a regular monitoring of genetic variability of the population is important and must be adopted, in order to avoid the danger of an excessive increase of inbreeding in the future, which would result in significant inbreeding depression and in significant loss of genetic variation.

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Section: Introductionmentioning

confidence: 74%

Genetic Variability inBracco ItalianoDog Breed Assessed by Pedigree Data

Cecchi

Paci

Spaterna

et al. 2013

Italian Journal of Animal Science

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“…; Ciampolini et al . ). Recently, however, for production animals such as pigs ( Sus scrofa ) and cattle ( B. taurus ), a trend towards identification and parentage verification using single nucleotide polymorphisms (SNPs) has been observed (Rohrer et al .…”

Section: Discussionmentioning

confidence: 97%

An evaluation of the International Society for Animal Genetics recommended parentage and identification panel for the domestic pigeon (Columba livia domestica)

Groot¹,

Haeringen²

2017

Animal Genetics

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In this study, the International Society for Animal Genetics (ISAG) recommended panel for the identification of the domestic pigeon (Columba livia domestica) is characterized based on commonly used statistical parameters. The marker panel is based on 16 short tandem repeat (STR) loci (PIGN15, PIGN10, PIGN57, PIGN26, CliμD16, CliμD19, PIGN12, CliμD17, CliμT17, PIGN04, CliμD01, CliμD11, CliμD35, CliμT02, CliμT13, CliμT43). The alleles of the 16 loci consist of a mixture of tri-, tetra-, penta- and hexameric repeat patterns. A sex determination marker was included in the multiplex for quality control. The repeat sequence of the PIGN markers was previously unpublished and therefore sequenced to reveal the sequence pattern. In total, 1421 pigeons were genotyped on 16 STR loci to generate allele frequency data for each locus. For all 16 markers combined, a PE1 (combined non-exclusion probability, first parent) of 0.9986 and PE2 (combined non-exclusion probability, second parent) of >0.9999 was observed. Comparing the alleged father and mother, a PE value of >0.9999 was observed. Two of the markers, CliμD19 and PIGN12, were found to have relatively high Hardy-Weinberg equilibrium and F(null) values. Therefore these markers may be considered to be replaced by other STRs. Another point of discussion may be to add a gender identification marker to the recommended ISAG panel. Not only can this serve as an extra identification marker, but this can also confirm the sex of a sample, because it is challenging to determine the sex based on phenotypical characteristics, especially for chicks. In conclusion, the set of 16 STR markers can be used in routine parentage verification and the identification of individuals.

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“…The 19 microsatellites used in the analysis (Table 1 ) were located on 18 of the 39 chromosomes of the dog genome ( Canis lupus familiaris ); they are included in the “core panel” of loci recommended by the international society for animal genetics (ISAG) for parentage verification in the international canine comparison test [ 15 ]. The 19 microsatellites were co-amplified in a single multiplex PCR reaction.…”

Section: Main Textmentioning

confidence: 99%

The use of genetic markers to estimate relationships between dogs in the course of criminal investigations

et al. 2017

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ObjectiveAttacks on humans by dogs in a pack, though uncommon, do happen, and result in severe, sometimes fatal, injuries. We describe the role that canine genetic markers played during the investigation of a fatal dog-pack attack involving a 50-year-old male truck driver in a parking lot in Tuscany (Italy). Using canine specific STR genetic markers, the local authorities, in the course of their investigations, reconstructed the genetic relationships between the dogs that caused the deadly aggression and other dogs belonging to the owner of the parking who, at the moment of the aggression, was located in another region of Italy.Results From a Bayesian clustering algorithm, the most likely number of clusters was two. The average relatedness among the dogs responsible for the aggression was higher than the average relatedness among the other dogs or between the two groups. Taken together, all these results indicate that the two groups of dogs are clearly distinct. Genetic relationships showed that the two groups of dogs were not related. It was therefore unlikely that the murderous dogs belonged to the owner of the parking lot who, on grounds of this and additional evidence, was eventually acquitted.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-017-2722-6) contains supplementary material, which is available to authorized users.

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Towards standardization of canine STRs: a proposed nomenclature for six markers from the ISAG comparison‐test panel

Cited by 4 publications

References 14 publications

Genetic Variability inBracco ItalianoDog Breed Assessed by Pedigree Data

Genetic Variability inBracco ItalianoDog Breed Assessed by Pedigree Data

An evaluation of the International Society for Animal Genetics recommended parentage and identification panel for the domestic pigeon (Columba livia domestica)

The use of genetic markers to estimate relationships between dogs in the course of criminal investigations

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