Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Streptomyces spp.

El-Abyad, M.S.; El-Sayed, Mostafa A.; El-Shanshoury, Abd El-Raheem R.; El-Sabbagh, Sabha M.

doi:10.1007/bf00016608

Cited by 115 publications

(59 citation statements)

References 19 publications

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“…as seed-coating. An increased availability of growth regulators produced by the organism was the reason attributed for the improvement in tomato growth (El-Abyad et al, 1993). Gopalakrishnan et al, 2012 investigated the PGP activities of the Streptomyces strains (CAI-21, CAI-26 and MMA-32) in rice grown under aerobic soil conditions by the SRI method and they reported that the Streptomyces strains significantly enhanced all the morphological parameters like plant height, tillering, panicle length and dry weight, filled grain number and weight, seed weight, stover and grain yield.…”

Section: Resultsmentioning

confidence: 99%

Biocontrol and growth promotive potential of Streptomyces spp. in black pepper (Piper nigrum L.)

Bhai

Lijina

Prameela

et al. 2017

JBC

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Actinomycetes isolated from the rhizosphere of black pepper and from vermicompost were tested for their antagonistic effect against Phytophthora capsici and Radopholus similis, the causal agents of foot rot and slow decline diseases of black pepper. Based on in vitro evaluations, four isolates were shortlisted (IISR Act2, IISR Act5, IISR Act6, and IISR Act9) and subjected to in vivo evaluation for Phytophthora infection by challenge inoculation and also greenhouse evaluation for growth promotion in black pepper. Rooted plants of black pepper were raised in soil amended with Actinomycetes strains individually and in combinations in portray and were transplanted into earthenware pots containing potting mixture amended with respective actinomycetes keeping un-amended plants as control. Observations were recorded on growth parameters like plant height, root weight, shoot weight and root infection by nematodes. Besides, soil was also analyzed for pH, dehydrogenase activity, EC and NPK content to know the influence of actinomycetes on soil microflora as well as on nutrient status. The results showed that consortia are more effective than individual isolates. Consortia holding IISR Act5+IISR Act9 were found highly effective in enhancing all the growth parameters followed by IISR Act2+ IISR Act9 and IISR Act2 + IISR Act5. The dehydrogenase activity was found higher in these consortia showing the higher microbial metabolic activity. Root lesions were also negligible in these treatments. Being effective in growth promotion as well as antagonistic activity, the isolates were tested for plant growth promotion and biocontrol traits. Among the isolates, IISR Act9 was found highly efficient in IAA production (119µg/ml) when compared to IISR Act2 (36.25µg/ml) and IISR Act5 (32.4µg/ml). Hence based on the growth promotive and pathogen suppressive effect, the consortia of either IISR Act5+IISR Act9 , IISR Act2+IISR Act9 or IISR Act2+IISR Act5 can be effectively used in black pepper for growth promotion and biological control of foot rot and slow decline diseases. The potential actinomycetes were identified as Streptomyces spp. as per Bergey's manual and rpoB gene sequence similarity of which IISR Act2 is identified as Streptomyces sp., IISR Act9 as Streptomyces albus and IISR Act5 as Streptomyces sp.

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Section: Resultsmentioning

confidence: 99%

Biocontrol and growth promotive potential of Streptomyces spp. in black pepper (Piper nigrum L.)

Bhai

Lijina

Prameela

et al. 2017

JBC

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“…mecanismos como produção de antibióticos, enzimas líticas e indução a resistência (El-Abyad et al, 1993;Samac & Kinkel, 2001). …”

Section: Resultsunclassified

Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Sousa

Soares

Garrido

et al. 2006

Pesq. agropec. bras.

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Resumo -Este trabalho teve como objetivo avaliar o efeito de seis isolados de estreptomicetos na mortalidade e eclosão de juvenis de segundo estádio (J2) de Meloidogyne incognita e no controle da meloidoginose em mudas de tomateiro. Foi montado um bioensaio em placas tipo Elisa, sendo adicionados em cada célula, 200 µL de metabólitos dos isolados, com 20 µL de uma suspensão com 25 juvenis de segundo estádio (J2) de M. incognita. Os metabólitos produzidos por Streptomyces griseus subsp. griseus causaram 98,2% de mortalidade dos J2 de M. incognita. Em outro bioensaio, foram adicionados 3 mL dos metabólitos em frascos de vidro, com 100 µL da suspensão contendo 25 ovos de M. incognita. O isolado N0035 de Streptomyces proporcionou 98,8% de inibição na eclosão de J2 de M. incognita. Num terceiro bioensaio, o substrato de produção de mudas foi infestado com suspensão de estreptomicetos e incubado por 30 dias. Quinze dias depois da germinação das sementes do tomateiro, foi realizada a inoculação com 2.000 J2 por planta. Verificou-se a redução de 68% no número de galhas por grama de raiz e de 76,8% na massa de ovos por grama de raiz, nas mudas produzidas no substrato infestado e incubado com Streptomyces griseus subsp. griseus, quando comparado com a testemunha.Termos para indexação: Meloidogyne incognita, Lycopersicon esculentum, Streptomyces, actinomicetos, nematóide-das-galhas, biocontrole. Streptomycetes in the control of Meloidogyne incognita in tomato plantsAbstract -This work aimed to evaluate the effect of six streptomycete isolates on mortality, hatching, and control of Meloidogyne incognita on tomato seedlings. An assay was conducted in Elisa plates, with the addition of 200 µL of the streptomycete metabolites, along with 20 µL of suspension containing 25 second stage juveniles (J2) of M. incognita. The metabolites produced by Streptomyces griseus subsp. griseus caused mortality of 98.2% on M. incognita. A second assay was conducted with 3 mL of streptomycete metabolites and 25 eggs of M. incognita. The isolate N0035 of Streptomyces caused inhibition of 98.8% on hatching. In a third assay, potting mix was infested with suspension of the streptomycetes isolates and incubated for 30 days. Fifteen days after germination, tomato seedlings were inoculated with a suspension containing 2,000 J2 of M. incognita. Reductions of 68% in the number of galls per gram of roots and of 76.8% in the egg mass per gram of roots were observed on tomato seedlings grown in the potting mix inoculated with Streptomyces griseus subsp. griseus, compared to the control.

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“…O controle biológico pela introdução de microrganismos antagônicos a R. solanacearum, incluindo-se estirpes avirulentas, pode não resultar no controle do fitopatógeno. Por outro lado, a utilização de isolados de estreptomicetos tem apresentado resultados mais promissores (El-Abyad et al, 1993).…”

Section: Introductionunclassified

Seleção de isolados de estreptomicetos para controle de Ralstonia solanacearum em tomateiro

Gava

Pereira²,

Fernandes

et al. 2002

Pesq. agropec. bras.

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Resumo -Em condições tropicais, a murcha-bacteriana, causada por Ralstonia solanacearum, tem provocado danos severos à cultura do tomate (Lycopersicon esculentum Mill.), principalmente em condições de temperaturas acima de 25°C, com umidade relativa elevada. Neste contexto, o controle biológico pode representar uma alternativa viável. Este trabalho teve como objetivo selecionar isolados de estreptomicetos para o controle de R. solanacearum em tomateiro. Entre os isolados avaliados verificaram-se diferenças na inibição do patógeno in vitro, tanto em relação à faixa de pH avaliada, quanto ao tempo de observação. Constataram-se, também, variações no estabelecimento dos isolados de estreptomicetos na rizosfera das plântulas e nas mudas preparadas para transplante, e os isolados SP164, SF232, SAc326 e SG384 apresentaram as densidades populacionais mais elevadas. O experimento conduzido em canteiros com solo infestado com R. solanacearum mostrou que as plantas tratadas com o isolado SG384 apresentaram melhor nível de controle, 48 dias após a semeadura, período no qual todas as testemunhas haviam morrido.Termos para indexação: Lycopersicon esculentum, controle biológico, inoculação, rizosfera. Selection of streptomycetes isolates for the control of Ralstonia solanacearum in tomato plantsAbstract -The bacterial wilt caused by Ralstonia solanacearum causes high damages in tomato (Lycopersicon esculentum Mill.) culture, mainly under temperature above 25 o C and high relative humidity. Because conventional methods of control have shown low efficiency, biological control is a promising alternative. This work aimed to select isolates of streptomycetes for R. solanacearum control in tomato. Differences were found among the isolates in the in vitro inhibition of the pathogen either in time or pH range. The streptomycetes isolates grew differently in the rhizosphere of seedlings and plantlets of tomato. The isolates SP164, SF232, SAc326 and SG384 showed the highest population densities. In the experiment conducted in soil infested with R. solanacearum, plants treated with the isolate SG384 presented best control level 48 days after sowing, period after which all control plants were dead.

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Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Streptomyces spp.

Cited by 115 publications

References 19 publications

Biocontrol and growth promotive potential of <i>Streptomyces</i> spp. in black pepper (<i>Piper nigrum</i> L.)

Biocontrol and growth promotive potential of <i>Streptomyces</i> spp. in black pepper (<i>Piper nigrum</i> L.)

Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Seleção de isolados de estreptomicetos para controle de Ralstonia solanacearum em tomateiro

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