Towards the design of universal immunosurfaces for SPR-based assays: A review

Mauriz, Elba; García-Fernández, M. C.; Lechuga, Laura M.

doi:10.1016/j.trac.2016.02.006

Cited by 70 publications

(55 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Exploitation of the gold-thiol bond makes this immobilization strategy useful for gold substrates and nanoparticles in techniques such as surface plasmon resonance (SPR). 55 UV-excitation has also been used to initiate photoreduction of disulfide bridges in hinge regions of antibodies according to an approach known as the photonic immobilization technique. 56 Employing UV pulses at 258 nm and 10 kHz, free thiols can be produced that are then able to bind gold substrates for quartz crystal microbalance (QCM) measurements.…”

Section: Thiol Groupsmentioning

confidence: 99%

See 1 more Smart Citation

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

et al. 2017

View full text Add to dashboard Cite

Orientation of surface immobilized capture proteins, such as antibodies, plays a critical role in the performance of immunoassays. The sensitivity of immunodiagnostic procedures is dependent on presentation of the antibody, with optimum performance requiring the antigen binding sites be directed toward the solution phase. This review describes the most recent methods for oriented antibody immobilization and the characterization techniques employed for investigation of the antibody state. The introduction describes the importance of oriented antibodies for maximizing biosensor capabilities. Methods for improving antibody binding are discussed, including surface modification and design (with sections on surface treatments, three-dimensional substrates, self-assembled monolayers, and molecular imprinting), covalent attachment (including targeting amine, carboxyl, thiol and carbohydrates, as well as “click” chemistries), and (bio)affinity techniques (with sections on material binding peptides, biotin-streptavidin interaction, DNA directed immobilization, Protein A and G, Fc binding peptides, aptamers, and metal affinity). Characterization techniques for investigating antibody orientation are discussed, including x-ray photoelectron spectroscopy, spectroscopic ellipsometry, dual polarization interferometry, neutron reflectometry, atomic force microscopy, and time-of-flight secondary-ion mass spectrometry. Future perspectives and recommendations are offered in conclusion.

show abstract

Section: Thiol Groupsmentioning

confidence: 99%

“…Upon absorption on the sensing surface, the refractive index of the material changes causing the reflected light angle and the surface plasmon resonance angle to change accordingly. A recent review by Mauriz et al 55 covers SPR-based assays in great detail.…”

Section: Surface Plasmon Resonancementioning

confidence: 99%

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Among different sensing strategies, surface plasmon resonance (SPR) has been recognized one effective tool to combine with the immunosensing technique due to the characteristics of label-free, real-time, regeneration, and low cost (Mauriz et al 2016;Liang et al 2016). SPR immunosensor (Onodera and Toko 2014;Abadian et al 2014) can accurately monitor the changes in the refractive index caused by the binding reaction of antigen-antibody on the surface of the chip in real time Luz et al 2015), which made it have a significant advantage in rapid, selective and accurate detection of harmful substances, especially the small molecules in complex sample system.…”

Section: Introductionmentioning

confidence: 99%

Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor

Pan

Wang

et al. 2016

Food Anal. Methods

View full text Add to dashboard Cite

In this research, a reproducible surface plasmon resonance (SPR) immunosensor based on inhibition format was fabricated for stable and sensitive detection of four sulfonamides (SAs) in animal-derived foods. The parameters in the fabrication and measurement process were optimized and discussed in details. The method using the proposed SPR immunosensor was validated and exhibited favorable performance for SAs residues detection in common animal-derived food products, as well as acceptable accuracy (pure milk 88.5-106.2%; egg 89.0-91.5%; chicken muscle 87.2-94.7%; beef 84.8-96.9%; fish 87.8-95.0%), precision (relative standard deviation (n = 3), pure milk 3.2-5.2%; egg 1.1-5.4%; chicken muscle 1.1-5.9%; beef 2.5-7.1%; fish 4.6-5.3%), and sensitivity (IC 15 , pure milk 59.6 ng mL − 1 , egg 56.1 ng mL − 1 , chicken muscle 66.9 ng mL −1 , beef 63.3 ng mL −1 , fish 62.7 ng mL −1 ). Each detection cycle could finish in less than 5 min, and each SPR chip could reuse 300 analysis cycles with response attenuation of 3.7%. These results have demonstrated that the proposed SPR immunosensor has offered an effective, accurate, sensitive, rapid, and low-cost methodology for SAs residue detection in food sample, and this method has great potential for the routine analysis of large numbers of samples on measuring different kinds of compounds.

show abstract

“…1 The basis of the SPR technique is the changes in the interfacial refractive index of a sensor surface due to the high-affinity interaction between an immobilized biomolecule and its biospecific analyte in solution. 2 SPR provides the evaluation of the analyte-immobilized ligand interaction as well as antigen–antibody (Ag-Ab) interaction without any needs for further chemical or biological markers.…”

mentioning

confidence: 99%

“…To acquire a detectable signal in an enzyme-linked immunosorbent assay (ELISA), Abs need to be labeled while SPR permits direct detection in real-time without any labels or additional steps. 1,3 …”

mentioning

confidence: 99%

Tips on ligand immobilization and kinetic study using surface plasmonresonance

Dolatabadi

Guárdia

2016

Bioimpacts

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) technique offers a robust label-free approach applicable in various investigations including binding affinity, specificity and kinetics of biological macromolecules (e.g., peptides, proteins and nucleotidase) and small molecules. SPR provides extremely important data on the kinetics and affinity of substances examined, through which bio-specific interaction(s) can be established by the analysis of adsorption of analyteonto the immobilized ligand(s) on a sensor-based analytical system. Due to SPR wide applications in biomedical laboratories, the aim of this editorial is to highlight the importance of SPR in affinity kinetics and ligand immobilization.

show abstract

Towards the design of universal immunosurfaces for SPR-based assays: A review

Cited by 70 publications

References 73 publications

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor

Tips on ligand immobilization and kinetic study using surface plasmonresonance

Contact Info

Product

Resources

About