Towards The Isolation Of Sperm Cells For Androgenic Purposes

Keijzer, C.J.; Wilms, H.J.; Aelst, A.C. van; Klooster, H.B. Leferink-ten

doi:10.1515/9783110871944-052

Cited by 3 publications

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“…Solutions of different osmotic values, ranging from pure water (Keijzer et al 1986) to 20% sucrose (Russell 1986), have been used to induce the bursting of pollen grains. Small grains, however, can resist incubation in pure water (Theunis et al 1988).…”

Section: Methodsmentioning

confidence: 99%

Isolation of male and female gametes in higher plants

Theunis

Pierson

Cresti

1991

Sexual Plant Reprod

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The availability of generative cells, sperm cells, embryo sacs and egg cells from angiosperm plants in isolated conditions has opened up many prospects: study of the mechanism of recognition and fusion between gametes of opposite sex and detailed observation of the process of fertilization, biochemical and genetic analysis of gamete-specific components and genetic engineering in combination with in vitro fertilization. This review provides a list of about ninety publications, in which the isolation of male or female angiosperm gametes and the closely related generative cells and embryo sacs is reported. The species used are summarized in two tables. A description is given of the diverse isolation techniques, which consist of enzymatic digestion, bursting of pollen by osmotic shock, squashing, grinding and micro-dissection. Viability of isolated cells and yield, two important aspects of biotechnological manipulation, are emphasized. A critical evaluation of the most significant results obtained so far with isolated material is presented together with notes on prospects for the future.

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Section: Methodsmentioning

confidence: 99%

Isolation of male and female gametes in higher plants

Theunis

Pierson

Cresti

1991

Sexual Plant Reprod

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Tissue interactions in the developing loculeof Gasteria verrucosaduring microgametogenesis

Keijzer

Willemse

1988

Acta Botanica Neerlandica

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S UMM A RYWe investiga ted th e develo pment of the locule ti ss ues of Gasteria verrucosa (Mill.) H. Du va l using electron microscopy a nd summ a rized it in deta iled pen-d ra,wings .Most of the a nth er o pening processes ta ke pl ace in th e epidermi s a nd th e end o thecium . The sta rch in these two layers is used fo r different steps o f the dehi scence process. The (sin gle) middle laye r is iso lated from the end o thecium a nd fin a lly degenera tes. This process is rel ated to ta peta l fun cti ons.Spo ro poll enin -co ntainin g structures a ri se on both the tapetum a nd the microspo res, fo r po llen di spersa l a nd pro tecti o n, respec ti vely. In the ta petum , sta rch turn s into part of the po llenkitt. During the micros pore mitosis the sta rch of the micros po res is deposited in the vegeta ti ve cell. The po la rity in th e microspo res is rela ted to th e fo rm a ti o n a nd develo pm ent of the genera ti ve cell . Ultrastructura l cha nges in the different cell layers a re di scussed in rela ti o n to their develo pment a nd interacti o n.Key-words: a nther, a nther dehi scence, end o thecium, po llen, po ll en di spersa l, tapetum , ti ssue, interaction.I NT RODU C TIO N

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Three-dimensional observations on freeze-fractured frozen hydratedPapaver dubiumpollen with cryo-scanning electron microscopy

Aelst

Mueller

Dueggelin

et al. 1989

Acta Botanica Neerlandica

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The ultrastructure of generative cells and vegetative cells of Papaver dubium L. pollen was studied with low-temperature scanning electron microscopy (LTSEM). Liquid propane freezing resulted in a very fast fixation. The on-line fracturing and observation of the pollen grains was performed at -130 a C. The specimens were fully hydrated and did not undergo morphological changes during the procedure. Unfractured and cross fractured organelles, generative cells, vegetative nuclei and membranes are visible in three-dimensional images. The LTSEM micrographs show a lobed vegetative nucleus with many nuclear pores and a wrinkled generative cell in longitudinal direction. The vegetative nucleus and the generative cell are situated close to each other but structural connections are not observed.

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Towards The Isolation Of Sperm Cells For Androgenic Purposes

Cited by 3 publications

References 0 publications

Isolation of male and female gametes in higher plants

Isolation of male and female gametes in higher plants

Tissue interactions in the developing loculeof Gasteria verrucosaduring microgametogenesis

Three-dimensional observations on freeze-fractured frozen hydratedPapaver dubiumpollen with cryo-scanning electron microscopy

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