Towards the protein phosphatase-based biosensor for microcystin detection

Campàs, Mònica; Szydlowska, Dorota; Trojanowicz, Marek; Marty, Jean‐Louis

doi:10.1016/j.bios.2004.06.002

Cited by 62 publications

(37 citation statements)

References 39 publications

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“…5B). The detection limit for MC-LR obtained by the proposed method compares favorably with other electrochemical biosensors [47,48]. In terms of the sources of interference, the proposed potentiometric assay is better suited for colored and turbid samples (Table 1).…”

Section: Determination Of Mc-lrmentioning

confidence: 83%

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Ding

Wang

et al. 2016

Sensors and Actuators B: Chemical

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a b s t r a c tWe report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 g/L with a detection limit of 0.5 g/L (3 ). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition.

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Section: Determination Of Mc-lrmentioning

confidence: 83%

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Ding

Wang

et al. 2016

Sensors and Actuators B: Chemical

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“…Furthermore, a comparison of the other biosensors for MCs detection is summarized in Table 1, in contrast to the other techniques such as the electrochemical and evanescent wave allfiber biosensors for MCs detection [7,9,[11][12][13], the present SPR immunosensor was not as highly sensitive as the biosensors above, which may be due to the different antibodies, assay strategies, and technique platforms. However, there is still further scope of the SPR immunoassay sensitivity improvement since antibodyamplification strategies have been successfully applied in other biochemical molecules analyses [21,22].…”

Section: Detection Of Mcs Using Spr Biosensor and Regeneration Efficimentioning

confidence: 92%

“…However, these methods require either complex operation or expensive materials and devices. To circumvent these drawbacks, several research groups have made the attempts to develop biosensors for MCs detection, such as molecularly imprinted polymer (MIP)-based sensor [5], electrochemical immunosensors [6][7][8][9], and evanescent wave all-fiber immunosensors [10,11]. There have been the reports that described the application of test strips for rapid MCs analysis in situ [12,13]; however, it is still challenging to develop a simple, rapid, and sensitive method for MCs analysis.…”

Section: Introductionmentioning

confidence: 99%

Detection of microcystins in environmental samples using surface plasmon resonance biosensor

Gan

Chen

et al. 2009

Talanta

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“…Several additives can be added to the sol-gel in different phases of the preparation, resulting in "tailored materials" suitable for a number of proteins and detection systems [50]. Table 1 summarizes some applications of silica gel-encapsulated proteins reported in the literature [183,194,[200][201][202][203][204][205][206][207][208][209][210][211][212][213][214][215][216][217]. Notable examples include cases in which more proteins are co-encapsulated to immobilize short metabolic pathways [194].…”

Section: Proteins Encapsulated In Silica Gel As Biosensorsmentioning

confidence: 99%

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

Ronda

Bruno

Campanini

et al. 2015

COC

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Abstract:The development of silica-based sol-gel techniques compatible with the retention of protein structure and function started more than 20 years ago, mainly for the design of biotechnological devices or biomedical applications. Silica gels are optically transparent, exhibit good mechanical stability, are manufactured with different geometries, and are easily separated from the reaction media. Biomolecules encapsulated in silica gel normally retain their structural and functional properties, are stabilized with respect to chemical and physical insults, and can sometimes exhibit enhanced activity in comparison to the soluble form. This review briefly describes the chemistry of protein encapsulation within the pores of a silica gel three-dimensional network, the mechanism of interaction between the protein and the gel matrix, and its effects on protein structure, function, stability and dynamics. The main applications in the field of biosensor design are described. Special emphasis is devoted to silica gel encapsulation as a tool to selectively stabilize subsets of protein conformations for biochemical and biophysical studies, an application where silica-based encapsulation demonstrated superior performance with respect to other immobilization techniques.

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Towards the protein phosphatase-based biosensor for microcystin detection

Cited by 62 publications

References 39 publications

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Detection of microcystins in environmental samples using surface plasmon resonance biosensor

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

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