Toxaphene affects the levels of mRNA transcripts that encode antioxidant enzymes in Hydra

Woo, Seonock; Lee, Aekyung; Won, Hyokyoung; Ryu, Jae‐Chun; Yum, Seungshic

doi:10.1016/j.cbpc.2012.03.005

Cited by 12 publications

(11 citation statements)

References 29 publications

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“…The cDNA fragments of 10 genes, obtained from the conversion of the Hydra circumcincta RNAs, were amplified by qRT-PCR, with dedicated sets of primer for each gene, as described by Woo et al (2012). The studied genes were the genes coding for : Tubulin α-1, as housekeeping gene (TUBA1, GenBank accession no.…”

Section: Expression Of Some Stress Gene Evaluated By Means Of Qrt-pcrmentioning

confidence: 99%

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Multi-scale impact of chronic exposure to environmental concentrations of chlordecone in freshwater cnidarian, Hydra circumcincta

Colpaert¹,

Villard²,

Jong³

et al. 2020

Environ Sci Pollut Res

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Chlordecone (CLD) is an organochlorine pesticide widely used by the past to control pest insects in banana plantations in the French West Indies. Due to its persistence in the environment, CLD has contaminated the soils where it has been spread, as well as the waters, and is still present in them. The objective of our study was to evaluate the effects of chronic exposure to environmentally relevant CLD concentrations in an animal model, the freshwater hydra (Hydra circumcincta). In a multi-marker approach, we have studied the expression of some target stress genes, the morphology, and the asexual reproduction rates. Our data showed that exposure to low concentrations of chlordecone leads to i) a modulation of the expression of target genes involved in oxidative stress, detoxification, and neurobiological processes, and ii) morphological damages and asexual reproduction impairment. We have observed non-monotonic dose-response curves, which agree with endocrine disrupting chemical effects. Thus, "U-shaped" dose-response curves were observed for SOD, GRed, Hym355, and potentially GST gene expression; inverted "U-shaped" curves for GPx and CYP1A gene expression, and reproductive rates; as well as a biphasic dose-response curve for morphological damages. Therefore, in the range of environmental concentrations tested, very low concentrations of CLD can produce equally or more important deleterious effects than higher ones. Finally, to our knowledge, this study is the first one to fill the lack of knowledge concerning the effects of CLD in Hydra circumcincta, and confirms that this diploblastic organism is a pertinent freshwater model in the risk assessment.

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Section: Expression Of Some Stress Gene Evaluated By Means Of Qrt-pcrmentioning

confidence: 99%

“…Finally, the evaluation by qRT-PCR analyses of the expression of some stress genes can be easily performed and is inexpensive (Woo et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Multi-scale impact of chronic exposure to environmental concentrations of chlordecone in freshwater cnidarian, Hydra circumcincta

Colpaert¹,

Villard²,

Jong³

et al. 2020

Environ Sci Pollut Res

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“…Antibody staining and in situ hybridization detected the peptide in the neurons in the tentacles and around the mouth, as well as in the lower part of the peduncle and the basal disk. We found that the transcription level of the gene encoding Hym-355 was upregulated by toxaphene exposure 40 . Thus, newly differentiated neurons may be necessary to compensate for the neurons damaged under the acute neurotoxic conditions caused by toxaphene.…”

Section: Nervous Systemmentioning

confidence: 98%

“…The authors expected that HyAChE would have an alternative function in Hydra, apart from its function in neurotransmission, because the nonneuronal cholinergic system in the epithelium is prominent in Hydra. This could explain why the transcription levels of HyAChE were not affected by toxaphene exposure 40 .…”

Section: Nervous Systemmentioning

confidence: 98%

“…Many toxicants induce oxidative stress by disrupting the equilibrium between the production of reactive oxygen species (ROS) and their efficient removal by antioxidant systems. We established a real-time quantitative PCR system to detect the transcript level changes in six antioxidant enzyme genes, encoding catalase, glucose-6-phosphate dehydrogenase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, and superoxide dismutase, in H. magnipapillata 40 . The response of Hydra to toxaphene-induced oxidative stress was deduced from the altered expression levels of these antioxidant enzyme genes.…”

Section: Oxidative Stressmentioning

confidence: 99%

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Hydra, a candidate for an alternative model in environmental genomics

Yum

Woo

Lee

et al. 2014

Mol. Cell. Toxicol.

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The need for nonmammalian and nonvertebrate experimental systems for toxicology is increasing because of growing concern about animal welfare. Hydra, a genus of freshwater cnidarian, has been used in classical toxicology for more than 40 years and has been recommended as a standard model for ecotoxicology. Functional assays using Hydra homologues of the signal molecules and transcription factors of other metazoans have demonstrated that Hydra shares the common molecular mechanisms for pattern formation with bilaterian animals. The availability of the Hydra genome and mRNA information has meant that it can be used as a model organism in biological sciences. The purpose of this review is to explore recent developments in our understanding of the molecular aspects of axis formation, bud formation, regeneration, and the nervous system in Hydra and to introduce Hydra as an alternative animal model in environmental toxicogenomics for predicting the toxicity of environmental pollutants at the molecular level.

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Futuristic Approach to Alternative Model Organisms: Hydra Stakes Its Claim

Anbazhagan

Zeeshan

Akbarsha

2018

Alternatives to Animal Testing

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The use of mammalian models for toxicological risk assessment of chemicals has been a widespread practice for well over 50 years. This has remained controversial in view of the species differences. Further, the advances in molecular biology techniques have revolutionized the toxicological risk assessment scenario by creating demand for rapid, less expensive and highly relevant methods to risk-evaluate chemical substances quickly. In order to take advantage of these advances the US EPA funded the National Research Council to develop a strategy and vision for toxicity testing in 21 st century (Tox21). Tox21 envisions a paradigm shift in toxicity testing and proposes the use of emerging technologies based on non-animal methods for better understanding of chemical-environment interaction, and also emphasizes the adoption of animals belonging to lower levels of taxonomic hierarchy, which are less sentient, in in vivo toxicity testing. Adoption of this shift would perhaps benefit translation of the REACH Legislation, which otherwise greatly relies on animal experiments for chemical risk assessment. We aimed at establishing Hydra, a simple organism belonging to Phylum Cnidaria, as a model organism for toxicity testing of environmental chemicals. Besides, Hydra offers advantages such as easy to culture, reproduces fast, cost-effective and highly sensitivity to inorganic pollutants. Moreover, the whole genome sequence of hydra revealed that most of the genes are conserved in which sense it offers advantage over even in the most routinely used other invertebrate model organisms. We standardized several assay methods which formed the nucleus of several publications. Herein we show that Hydra can be used as a viable bio-indicator for early warning of aquatic pollutants.

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Toxaphene affects the levels of mRNA transcripts that encode antioxidant enzymes in Hydra

Cited by 12 publications

References 29 publications

Multi-scale impact of chronic exposure to environmental concentrations of chlordecone in freshwater cnidarian, Hydra circumcincta

Multi-scale impact of chronic exposure to environmental concentrations of chlordecone in freshwater cnidarian, Hydra circumcincta

Hydra, a candidate for an alternative model in environmental genomics

Futuristic Approach to Alternative Model Organisms: Hydra Stakes Its Claim

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