Toxic Activity of Purified Lipopolysaccharide of Neisseria gonorrhoeae for Human Fallopian Tube Mucosa

Gregg, Clark R.; Melly, M. Ann; Hellerqvist, Carl G.; Coniglio, John G.; McGee, Zell A.

doi:10.1093/infdis/143.3.432

Cited by 126 publications

(59 citation statements)

References 31 publications

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“…In this model it was shown that GC are internalized by nonciliated cells and sequestered within membrane-bound vacuoles which transcytose, fuse with the cells' basolateral membranes, and release the bacteria into the submucosa (22,26,(39)(40)(41)80), where they can initiate an inflammatory reaction and invade the bloodstream to spread systemically.…”

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confidence: 99%

Effect of α-Oligosaccharide Phenotype of Neisseria gonorrhoeae Strain MS11 on Invasion of Chang Conjunctival, HEC-1-B Endometrial, and ME-180 Cervical Cells

2000

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The genes encoding the glycosyltransferases responsible for the addition of the five sugars in the ␣-oligosaccharide (␣-OS) moiety of lipooligosaccharide (LOS) have been identified. Disruption of these glycosyltransferase genes singly or in combination results in corresponding truncations in LOS. In the present work we show that sequential deletion of the terminal four sugar residues of gonococcal ␣-OS had no discernible effect on the invasion of human conjunctival, endometrial, and cervical cell lines. However, deletion of the proximal glucose, which resulted in the complete deletion of ␣-OS, significantly impaired invasion of the gonococci into all three cell lines. The effect of deleting ␣-OS on invasion was independent of and additive to the known invasion-promoting factor OpaA. These data suggest that the proximal glucose residue of the ␣-OS chain of LOS is required for efficient invasion of gonococci into host mucosa.

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confidence: 99%

Effect of α-Oligosaccharide Phenotype of Neisseria gonorrhoeae Strain MS11 on Invasion of Chang Conjunctival, HEC-1-B Endometrial, and ME-180 Cervical Cells

2000

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“…injluenzae endotoxin. It is interesting that sterile supernatant fluids from fallopian tube organ cultures infected with Neisseria gonorrhoeae similarly cause loss of ciliary activity and sloughing of epithelial cells when transferred to fresh organ cultures Johnson et al, 1977) and Gregg et al (1981) have shown that the active factor in such supernatant fluids is gonoccocal lipopolysaccharide (endotoxin). The role that H .…”

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Scanning Electron Microscopy of the Interaction between Haemophilus influenzae and organ cultures of rat trachea

Johnson

Clark

Osborn

1983

Journal of Medical Microbiology

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“…It is an outer membrane glycolipid involved in immune system evasion, attachment to epithelial tissue, host cell invasion, mediation of toxic damage in the fallopian tube, and stimulation of the production of bactericidal antibodies (2,15,20,41,44,45). Biosynthesis of gonococcal and meningococcal LOS occurs via the same biosynthetic pathway producing a branched oligosaccharide attached to lipid A via two 3-deoxy-D-manno-2-octulosonic acid (KDO) molecules (12,13,21,22,48).…”

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Analysis of Lipooligosaccharide Biosynthesis in the Neisseriaceae

2001

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Neisserial lipooligosaccharide (LOS) contains three oligosaccharide chains, termed the ␣, ␤, and ␥ chains. We used Southern hybridization experiments on DNA isolated from various Neisseria spp. to determine if strains considered to be nonpathogenic possessed DNA sequences homologous with genes involved in the biosynthesis of these oligosaccharide chains. The presence or absence of specific genes was compared to the LOS profiles expressed by each strain, as characterized by their mobilities on sodium dodecyl sulfatepolyacrylamide gel electrophoresis gel and their reactivities with various LOS-specific monoclonal antibodies. A great deal of heterogeneity was seen with respect to the presence of genes encoding glycosyltransferases in Neisseria. All pathogenic species were found to possess DNA sequences homologous with the lgt gene cluster, a group of genes needed for the synthesis of the ␣ chain. Some of these genes were also found to be present in strains considered to be nonpathogenic, such as Neisseria lactamica, N. subflava, and N. sicca. Some nonpathogenic Neisseria spp. were able to express high-molecular-mass LOS structures, even though they lacked the DNA sequences homologous with rfaF, a gene whose product must act before gonococcal and meningococcal LOS can be elongated. Using a PCR amplification strategy, in combination with DNA sequencing, we demonstrated that N. subflava 44 possessed lgtA, lgtB, and lgtE genes. The predicted amino acid sequence encoded by each of these genes suggested that they encoded functional proteins; however, structural analysis of LOS isolated from this strain indicated that the bulk of its LOS was not modified by these gene products. This suggests the existence of an additional regulatory mechanism that is responsible for the limited expression of these genes in this strain.

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Toxic Activity of Purified Lipopolysaccharide of Neisseria gonorrhoeae for Human Fallopian Tube Mucosa

Cited by 126 publications

References 31 publications

Effect of α-Oligosaccharide Phenotype of Neisseria gonorrhoeae Strain MS11 on Invasion of Chang Conjunctival, HEC-1-B Endometrial, and ME-180 Cervical Cells

Effect of α-Oligosaccharide Phenotype of Neisseria gonorrhoeae Strain MS11 on Invasion of Chang Conjunctival, HEC-1-B Endometrial, and ME-180 Cervical Cells

Scanning Electron Microscopy of the Interaction between Haemophilus influenzae and organ cultures of rat trachea

Analysis of Lipooligosaccharide Biosynthesis in the Neisseriaceae

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