The genotoxic effects of the herbicide oxyfluorfen on the root meristem cells of Heianthus annuus L. (sunflower) were investigated. The roots were treated with 75, 150, and 300 ppm concentrations of oxyfluorfen for 12 and 24 h. The different genetic endpoints such as the mitotic index (MI), chromosome aberrations (CA), and micronuclei (MN) were analyzed in both control and test groups. The oxyfluorfen showed a marked mitodepressive action on mitosis. Additionally, MN were observed in interphase cells. The types of chromosome aberrations included disturbed prophase, c-mitosis, stickiness, laggards, and chromatid bridges. A pronounced toxic effect was observed at all concentrations applied. The herbicide oxyfluorfen may have genotoxic and clastogenic effects on sunflowers.