Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias, Juan Manuel Pérez; Soloneski, Sonia; Nikoloff, Noelia; Natale, Guillermo Sebastián; Larramendy, Marcelo L.

doi:10.1016/j.ecoenv.2015.04.045

Cited by 52 publications

(41 citation statements)

References 59 publications

(85 reference statements)

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“…Additionally, NAs other than MN were also induced after exposure to 1.0 mg/L trichlorfon. This result agrees with previous reports that morphological NAs are indicators of genetic damage . Finally, the increase in the number of leukocytes induced by trichlorfon indicates that more leukocytes were pumped from the haematopoietic tissue into the peripheral blood to defend against the damage caused by environmental pollution.…”

Section: Discussionsupporting

confidence: 92%

“…MN analysis is widely used as a bioassay of genotoxicity to detect small chromosomal fragments produced by clastogens or vagrant chromosomes produced by aneugens . MN formation in circulating erythrocytes in amphibian tadpoles as a consequence of pesticide treatment has been well demonstrated in previous studies . Our results demonstrated the potential of low doses of trichlorfon to induce DNA damage in the erythrocytes of this species after exposure for 4 weeks, whereas 0.01 mg/L trichlorfon exerted a least genotoxic effect.…”

Section: Discussionsupporting

confidence: 87%

“…The adverse effects of pesticides on amphibians, especially larvae, should therefore be investigated in aquatic ecological risk assessments. Previous studies have stressed that the tadpoles can be used as reference organisms in assessing the risk effects induced by several emerging pollutants, including agrochemicals …”

Section: Introductionmentioning

confidence: 99%

“…In addition, the effects of environmental pollutants on amphibians vary considerably depending on the timing of exposure during the life cycle. Most toxicity studies are performed in specific periods of the life cycle, and exposure during development may seriously impact population dynamics by influencing characteristics such as growth and development …”

Section: Introductionmentioning

confidence: 99%

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Effects of low doses Trichlorfon exposure on Rana chensinensis tadpoles

Yang

et al. 2018

Environmental Toxicology

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Trichlorfon is an organophosphate insecticide widely used in aquaculture and agriculture. Little is known about the effects of long-term of low doses trichlorfon exposure on amphibians. In this study, we investigated the effects of low doses trichlorfon on Rana chensinensis tadpoles after exposure to 0.01, 0.1, and 1.0 mg/L trichlorfon for 2 and 4 weeks. Survival, growth, development and mortality were monitored regularly over the course of exposure. The results showed that trichlorfon led to a decrease in tadpole survival. Reductions in growth and disruptions to the development of tadpoles were observed in trichlorfon treatments. Morphological abnormalities of affected tadpoles included axial flexures, skeletal malformations and lateral kinks. Trichlorfon increased the frequency of micronucleus (MN) formation in circulating erythrocytes of tadpoles exposed for 2 weeks to 0.1 and 1.0 mg/L trichlorfon. At all concentrations, an enhanced frequency of MN formation was observed in tadpoles exposed for 4 weeks. Exposure to trichlorfon induced other nuclear abnormalities such as lobed and notched nuclei only in tadpoles exposed to 1.0 mg/L trichlorfon for 4 weeks. In addition, exposure to trichlorfon within the 0.01-1.0 mg/L range increased the genetic damage index in hepatic tissues in all treatments. Apoptosis-associated DNA fragmentation in hepatic tissues occurred in a weak ladder-like pattern. This study presents evidence of low doses trichlorfon effects on amphibians, highlighting the properties of this organophosphate insecticide that jeopardize nontarget species exposed to trichlorfon. K E Y W O R D S development, DNA damage, genotoxicity, tadpoles, trichlorfon

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of low doses Trichlorfon exposure on Rana chensinensis tadpoles

Yang

et al. 2018

Environmental Toxicology

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“…Boana pulchella tadpoles are a suitable in vivo model for assessing lethal and sublethal effects induced by several emerging pollutants, including insecticides (Natale et al 2018; Sansiñena et al 2018), herbicides (Peltzer et al 2013; Pérez‐Iglesias et al 2015; Sansiñena et al 2018), and heavy metals (Natale et al 2000, 2006). This species is easy to find in 2 well‐defined breeding season periods (winter and spring).…”

Section: Methodsmentioning

confidence: 99%

Sensitivity of Boana pulchella (Anura: Hylidae) Tadpoles to Environmentally Relevant Concentrations of Chlorpyrifos: Effects at the Individual and Biochemical Levels

Barreto

Costa

Demetrio

et al. 2020

Enviro Toxic and Chemistry

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We report sublethal effects of environmentally relevant concentrations of chlorpyrifos at the individual (swimming alterations) and biochemical (esterase activities and antioxidant enzymes) levels in the Montevideo tree frog Boana pulchella larvae. The 50% lethal concentration at 96 h (LC50‐96h) for chlorpyrifos in stage‐29 B. pulchella tadpoles was 0.98 mg/L, which was close to the 65th percentile of published anuran species sensitivity. In B. pulchella, chlorpyrifos disrupted biochemical processes: tadpoles showed a significant inhibition of esterase activity and a significant induction of antioxidant enzymes, indicating a response to an environmental challenge causing oxidative stress. Using principal components analysis, we could associate chlorpyrifos reduction in esterase activity with swimming alterations at 0.5 mg/L of the toxicant. The biochemical biomarkers reported in the present study respond at levels 20 times lower than the LC50‐96h and were associated with a biologically important response—swimming behavior. The link of responses across different levels of biological organization was demonstrated. The species is suitable as a model for ecotoxicological studies at different levels, including the individual and biochemical levels, and may be considered a good reference organism in environmental control programs. Environ Toxicol Chem 2020;39:834–841. © 2020 SETAC

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Application of Micronucleus Test in African Toad as Biomarker of Genotoxic and Cytotoxic Assessment of Contaminants in the African Environment

Alimba

2024

Biomonitoring of Pollutants in the Global South

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Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Cited by 52 publications

References 59 publications

Effects of low doses Trichlorfon exposure on Rana chensinensis tadpoles

Effects of low doses Trichlorfon exposure on Rana chensinensis tadpoles

Sensitivity of Boana pulchella (Anura: Hylidae) Tadpoles to Environmentally Relevant Concentrations of Chlorpyrifos: Effects at the Individual and Biochemical Levels

Application of Micronucleus Test in African Toad as Biomarker of Genotoxic and Cytotoxic Assessment of Contaminants in the African Environment

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