Toxic effect and mechanism of tris (1,3-dichloro-2-propyl)phosphate (TDCPP) on the marine alga Phaeodactylum tricornutum

Liu, Qian; Tang, Xuexi; Jian, Xiaoyang; Yang, Yingying; Ma, Wenqian; Wang, You; Zhang, Xinxin

doi:10.1016/j.chemosphere.2020.126467

Cited by 19 publications

(6 citation statements)

References 48 publications

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“…Before measurement, the mixture was centrifuged at 10,000 rpm for 15 min. Absorbance was measured at 663, 645, and 470 nm using a spectrophotometer . Pigment concentrations were calculated as C h l .25em a .25em ( normalm normalg / L ) = 15.65 A 666 − 7.34 A 653 C a r o t e n o i d s .25em ( normalm normalg / L ) = ( 1000 A 470 − 44.76 A 666 ) / 22 where A 666 , A 653 , and A 470 represent absorbance at 666, 653, and 470 nm, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…35 Photosynthetic Pigments. Photosynthetic pigments, including chlorophyll a (Chl a) and carotenoids, were measured at the end of the exposure experiment, as described by Liu et al 36 Briefly, 12 mL samples were collected and filtered through 0.22 μm filter membranes, which were then placed in a 50 mL centrifuge tube containing 5 mL methanol. 37 The centrifuge tube was shocked until the membrane was dissolved and then stored for 24 h at 4 °C.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Mao,

Ye,

Yang

et al. 2024

Environ. Sci. Technol.

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Antibiotics are being increasingly detected in aquatic environments, and their potential ecological risk is of great concern. However, most antibiotic toxicity studies involve single-exposure experiments. Herein, we studied the effects and mechanisms of repeated versus single clarithromycin (CLA) exposure on Microcystis aeruginosa. The 96 h effective concentration of CLA was 13.37 μg/L upon single exposure but it reduced to 6.90 μg/L upon repeated exposure. Single-exposure CLA inhibited algal photosynthesis by disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress and ultrastructural damage. In addition, CLA upregulated glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle. Repeated exposure caused stronger inhibition of algal growth via altering photosynthetic pigments, reaction center subunits biosynthesis, and electron transport, thereby inducing more substantial oxidative damage. Furthermore, repeated exposure reduced carbohydrate utilization by blocking the pentose phosphate pathway, consequently altering the characteristics of extracellular polymeric substances and eventually impairing the defense mechanisms of M. aeruginosa. Risk quotients calculated from repeated exposure were higher than 1, indicating significant ecological risks. This study elucidated the strong influence of repeated antibiotic exposure on algae, providing new insight into antibiotic risk assessment.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Mao,

Ye,

Yang

et al. 2024

Environ. Sci. Technol.

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“…The use of transcriptomics, independently and in combination with other types of analyses, can also shed light on adaptation of species through the discovery and identification of novel genes (Huang et al, 2019) or through revealing genetic variability amongst populations, like in Ribeiro et al, 2019 who paired transcriptomics with single-nucleotide polymorphism analysis. Furthermore, studies pairing transcriptomic technologies with other physiological or biological measurements allow for a multidimensional framework to address ecological hypotheses (e.g., Krol et al, 2020;Liu et al, 2020;Strader et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The Next Generation Is Here: A Review of Transcriptomic Approaches in Marine Ecology

Page

Lawley

2022

Front. Mar. Sci.

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As we enter the United Nations Decade of Ocean Science for Sustainable Development, extensive efforts to reverse the decline of ocean health are taking place. Moreover, the need to utilize innovative and integrative approaches to aid in these efforts and address marine ecological questions are urgent. Transcriptomic technologies provide tools to further our understanding of an organism’s biology by allowing researchers to rapidly gain information on the genetic variation of populations and the regulation of cellular processes and pathways through gene presence, absence, and expression. Here, we review the application of transcriptomics in the field of marine ecology over the last decade, following a systematic literature review approach. We found 478 articles that fit our search criteria of using transcriptomic approaches to address ecological hypotheses, with 70% of these studies occurring within the last 5 years. Among the analysed articles, 51.7% involved a type of stressor, 16.6% used transcriptomics to study adaptation, and another 15.9% researched ecological interactions. Most articles investigated species from kingdom Animalia, with a high representation from both molluscs (19.5%) and chordates (13.3%), and only 22% of studies had a fieldwork component. Our review demonstrates how the use of transcriptomic techniques in the field of marine ecology is increasing and how they are being applied. Although there are still challenges researchers experience using such techniques, particularly when annotating genes in non-model species and those with no prior genomic resources, these innovative technologies are extremely valuable in investigating differential gene expression, molecular pathways, and generating genomic resources.

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“…But the more toxicity effect of OPFRs on organisms is still an issue that deserves much attention, especially in plants. Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) caused morphological damage and growth inhibition of Phaeodactylum tricornutum and a decline in pigments and photosynthetic activity was observed, indicating the occurrence of photosynthesis inhibition (Liu et al 2020). However, few studies have investigated toxicity of OPFRs in plants.…”

Section: Introductionmentioning

confidence: 99%

Growth Hormone-Like Activities of Organophosphorus Flame Retardants (Opfrs) in Growth of Rice (Oryza Sativa L.)

Bai

Wang

Zhang

et al. 2021

Preprint

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To identify the toxic effect of Organophosphorus Flame Retardants (OPFRs) on plants, six different OPFRs involved in three concentrations (50 mg/L, 100 mg/L and 200 mg/L) were selected to estimate their toxicity to rice growth. The seed germination, seedling growth, OPFRs accumulation and antioxidative defense system were investigated in rice. The results showed that all of OPFRs inhibited the germination of seeds. OPFRs were accumulated in roots and shoots, and the accumulations of OPFRs in roots were higher than in shoots. However, the hormone-like effect of all six OPFRs was found on plants. The growth of seedlings was irrigated by 50 mg/L, 100 mg/L and 200 mg/L OPFRs in our experiment according to fresh weights and lengths of seedlings. And then malondialdehyde (MDA) and antioxidative defense system were impressed after OPFRs exposed 21 d. According to gene expressions of seven antioxidative enzymes at 14 d, most of enzyme expressions were updated to alleviated reactive oxygen species (ROS) by OPFRs exposure. Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) showed the strongest oxidative toxicity to plants among all of OPFRs. During the early 14 d period, the antioxidant enzymes could play important role in detoxification process. And after 21 d, antioxidants ascorbate (AsA) in roots and glutathione (GSH) in shoots could take over antioxidant enzymes against to OPFRs toxicity.

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Toxic effect and mechanism of tris (1,3-dichloro-2-propyl)phosphate (TDCPP) on the marine alga Phaeodactylum tricornutum

Cited by 19 publications

References 48 publications

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

The Next Generation Is Here: A Review of Transcriptomic Approaches in Marine Ecology

Growth Hormone-Like Activities of Organophosphorus Flame Retardants (Opfrs) in Growth of Rice (Oryza Sativa L.)

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