Toxic Effect of 2-ethyl (bicyclo[2.2.1] heptane) on Bacterial Cells

Манухов, И. В.; Yaguzhinsky, L. S.; Bermeshev, Maxim V.; Zisman, M.A.; Pevgov, V. G.; Samoilov, Vadim O.; Shorunov, Sergey V.; Максимов, А. Л.

doi:10.21519/0234-2758-2019-35-6-67-72

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…It is known that degrading of a number of hydrocarbon compounds leads to reactive oxygen species generation and oxidative stress induction in bacterial cells [25,26].…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

et al. 2020

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The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E . coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.

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“…It is known that degrading of a number of hydrocarbon compounds leads to reactive oxygen species generation and oxidative stress induction in bacterial cells [25,26].…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

et al. 2020

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“…It is known from the works [32,37] that strained hydrocarbon compounds are capable of causing DNA damage and oxidative stress, but not DNA alkylation in living cells. In E. coli cells, there are no homologues to the dinC gene of B. subtilis; therefore, the luxbiosensor E. coli pDinI, related to the SOS regulon and having a relatively high induction amplitude [28], was taken for comparison.…”

Section: Discussionmentioning

confidence: 99%

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

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Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H2O2. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.

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