Toxic effect of rifampicin on human osteoblast‐like cells

Abstract

We examined the effects of rifampicin on osteoblast-like cells derived from adult human bone in vitro. Cancellous bone was collected from five different individuals during elective orthopaedic operations and cultured in antibiotic-free media. Total DNA, 'H-thymidine incorporation and alkaline phosphatase (ALP) activity were measured after the cells were cultured for 4 days in media containing concentrations of rifampicin ranging from 0 to 1000 pg/ml. Mean total DNA was decreased at concentrations of 10 pglml and above in the cultures obtained from four out of five individuals but these decreases were significant in the cultures from only two individuals. 'H-thymidine incorporation, a more sensitive indicator of change in cell proliferation, and ALP activity were significantly decreased ( P < 0.05) in all of the cultures containing 3 and 7 pginil, respectively. In the clinical setting, serum concentrations of rifampicin often exceed 10 pglml after systemic administration. The present study has shown that rifampicin, at these concentrations. can inhibit the proliferation of osteoblast-like cells in vitro. Further studies should be carried out to assess whether rifampicin is detrimental to the bone repair process in vivo.

Effect of Local Rifamycin Application on Expression of BMP-2 and Bone Regeneration

“…22,23 On the other hand; previous studies and our study demonstrate higher osteoblast counts, enhanced bone formation 19 , high tolerance by bone 17,18 , and no histological damage. 16 Cytotoxicity may associated with dose, concentration, the type of antimicrobial and exposure time.…”

Efficacy of Ciprofloxacin-Releasing Bioabsorbable Osteoconductive Bone Defect Filler for Treatment of Experimental Osteomyelitis Due toStaphylococcus aureus

“…Fluoroquinolones have long been considered the drugs of choice for the treatment of chronic osteomyelitis because of their favorable penetration into poorly vascularized sites of infection, their advantageous bactericidal effects against all probable pathogens of chronic osteomyelitis, and the lack of serious adverse reactions (18,23). Similar to many antibiotics commonly used for the treatment of bone infections, such as rifampin and gentamicin (12,13), quinolones may have inhibitory effects on osteoblastic functions at high concentrations (10) and may delay bone healing (31).…”

A fully functional drug-eluting joint implant

“…Because of the potential inhibition of implant fixation due to high dose rifampin [43], we tested the effect of RVPE implants on the bony ongrowth to the surface of metal screws ( Methods ). To create the most clinically relevant model, the ratio of RVPE surface area to the bone-implant interface in the murine model was matched to those in the human knee (Supplementary Table 3).…”