Toxic effects of aging mask microplastics on E. coli and dynamic changes in extracellular polymeric matter

Jiang, Angrui; Pei, Wuxuan; Zhang, Rui; Shah, Kinjal J.; You, Zhaoyang

doi:10.1016/j.scitotenv.2023.165607

Cited by 8 publications

(1 citation statement)

References 53 publications

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“…For E. coli, we used the model of exponential plateau, whereas for S. epidermidis, we used the model of logistic growth. Additionally, according to the relevant bacterial studies of growth curves and some modifications, 21,45 values of maximum population in the fitting model were utilized to calculate bacterial growth inhibition (Text S6 and Table S4).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Toxicity Assessment of Environmental Liquid Crystal Monomers: A Bacteriological Investigation on Escherichia coli and Staphylococcus epidermidis

Huang,

Ruan,

Fang

et al. 2024

Environ. Sci. Technol.

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The widespread existence of liquid crystal monomers (LCMs) in various environmental matrices has been demonstrated, yet studies on the toxicological effects of LCMs are considerably scarce and are urgently needed to be conducted to assess the adverse impacts on ecology and human health. Here, we conducted a bacteriological study on two representative human commensal bacteria, Escherichia coli (E. coli) and Staphylococcus epidermidis (S. epidermidis), to investigate the effect of LCMs at human-relevant dosage and maximum environmental concentration on growth, metabolome, enzymatic activity, and mRNA expression. Microbial growth results exhibited that the highest inhibition ratio of LCMs on S. epidermidis reached 33.6% in our set concentration range, while the corresponding data on E. coli was only 14.3%. Additionally, LCMs showed more dose-dependent toxicity to S. epidermidis rather than E. coli. A novel in vivo solid-phase microextraction (SPME) fiber was applied to capture the in vivo metabolites of microorganisms. In vivo metabolomic analyses revealed that dysregulated fatty acid metabolism-related products of both bacteria accounted for >50% of the total number of differential substances, and the results also showed the species-specific and concentration-dependent metabolic dysregulation in LCM-exposed bacteria. The determination of enzymatic activity and mRNA relative expression levels related to oxidative stress confirmed our speculation that the adverse effects were related to the oxidative metabolism of fatty acids. This study complements the gaps in toxicity data for LCMs against bacteria and provides a new and important insight regarding metabolic dysregulation induced by environmental LCMs in human commensal bacteria.

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Section: ■ Results and Discussionmentioning

confidence: 99%