Toxic effects of chromatographic fractions ofPhyllanthus amarus on the serum biochemistry of rats

Adedapo, Adeolu Alex; Abatan, MO; Olorunsogo, Olufunso O.

doi:10.1002/ptr.1721

Cited by 22 publications

(17 citation statements)

References 10 publications

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“…Oral gavage of increasing concentrations of Moringa peregrina leaves ethanolic extracts for six days to mice significantly increased total WBCs numbers in the peripheral blood. Similar results were obtained with Moringa oleifera leaves ethanolic extract [ 8 , 9 ]. Such leucopoiesis effect could enhance immune response towards infectious agents.…”

Section: Discussionsupporting

confidence: 89%

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Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Al-Majali

Al-Oran

Hassuneh

et al. 2017

cejoi

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This study was conducted to assess the in vivo and ex vivo immunomodulatory effect of the ethanol leaves extract of Moringa peregrina in Balb/c mice. For this study, five groups of 5 Balb/c mice were given a single acute subtoxic oral dose of the ethanolic extract at 1.13, 11.30, 23.40 and 113.4 mg/kg and the immunomodulatory effect was assessed on the 6th day following the ingestion. In the (non-functional) assessment, the effect of the extract on the body weight, relative lymphoid organ weight, splenic cellularity and peripheral blood hematologic parameters were evaluated. While in the immunomodulation assessment (functional), we investigated the effect of the extract on the proliferative capacity of splenic lymphocytes and peripheral T and B lymphocytes using mitogen blastogenesis, mixed allogeneic MLR and IgM-Plaque forming cells assays. The ingestion of M. peregrina extract caused a significant increase in the body weight, weight and number of cells of spleen and lymph nodes of the treated mice. Furthermore, the count of RBCs, WBCs, platelets, hemoglobin concentration and PCV % were increased by the extract treatment in a dose-dependent manner. M. peregrina enhanced the proliferative responses of splenic lymphocytes for both T cell and B-cell mitogens. Likewise, the mixed lymphocyte reaction MLR assay has revealed a T-cell dependent proliferation enhancement in the extract treated mice. Moreover, the oral administration of M. peregrina leaves extracts significantly increased PFCs/106 splenocytes in a dose-dependent manner. In conclusion, subtoxic acute doses of M. peregrina extract demonstrated significant potential as an immunomodulatory agent even at the lowest dose of 1.13 mg/kg.

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Section: Discussionsupporting

confidence: 89%

“…Pre-treatment with Moringa extract inhibited cyclophosphamide bone marrow suppressive effect on phagocytic activity in mice [ 8 ]. Furthermore, various doses of Moringa oleifera caused a significant increase in the level of white blood cell counts and immunoglobulin levels [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Al-Majali

Al-Oran

Hassuneh

et al. 2017

cejoi

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“…The animals in the controlled experiment received only distilled water for the same number of days. The results revealed that some fractions of P. amarus had potentially deleterious effects on the blood and caused a significant increase in level of ALT, ALP, total bilirubinand blood urea nitrogen when compared with control (Adedapo et al, 2005a). The aqueous crude extract was administered orally to male rats of the Sprague Dawley strain in three groups receiving doses of 400, 800 and 1000 mg/kg but the fourth group served as a control and received distilled water only.…”

Section: Toxicological Assessment and Contraindicationsmentioning

confidence: 98%

Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review

Patel

Tripathi²,

Sharma

et al. 2011

Journal of Ethnopharmacology

288

183

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“…Body weight change of animals was also assessed, since changes in the body weights can be used as an indicator of adverse or toxic effects of drugs [ 16 , 17 ]. In this study, it was observed that the change in body weight of animals treated with the extract was not significant when compared to the control group, indicating absence of any severe adverse effects.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies

Adongo

Mante

Kukuia

et al. 2018

The Scientific World Journal

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Pseudospondias microcarpa is used traditionally for treating various diseases. However, although parts of the plant are extensively used in African traditional medicine, no scientific study has been reported on its toxicity. Therefore, this study evaluated the acute and subacute toxicity studies of the ethanolic extract of P. microcarpa in rats. Male Sprague-Dawley rats (120–150 g) were treated orally with the extract (30, 100, 300, 1000, and 3000 mg kg−1) or distilled water (10 ml kg−1) for 2 weeks and observed daily for general appearance and signs of toxicity. In addition, blood was collected for both biochemical and haematological assays. Sections of tissues from liver, kidney, spleen, brain, and stomach were also used for histopathological examination. Administration of the extract for 14 consecutive days caused no deaths, with an LD50 above 3000 mg kg−1. Except for lymphocytes (%) that showed a significant decrease (F5,23 = 3.93, P = 0.013), all other haematological parameters remained unaffected by the extract. The extract at 100 mg kg−1 showed a significant decrease in the levels of triglyceride and very-low-density lipoproteins (both at P < 0.05). Weight change as well as histological evaluation of the organs indicated no toxicity. The study demonstrates that an ethanolic extract of P. microcarpa given orally to rats is safe.

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Toxic effects of chromatographic fractions ofPhyllanthus amarus on the serum biochemistry of rats

Cited by 22 publications

References 10 publications

Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review

Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies

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