Toxic Effects of Detergents on the Corneal Endothelium

Nuyts, Rudy M. M. A.; Edelhauser, Henry F.; Pels, Elisabeth; Breebaart, A. C.

doi:10.1001/archopht.1990.01070100114045

Cited by 50 publications

(16 citation statements)

References 16 publications

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“…Nuyts et al described corneal changes of TECD patients whose corneal endothelium was destructed by intracameral injection of a detergent residue inside irrigation cannulas during cataract surgery. 14,15 In our case study, changes of the deep cornea, including endothelial cell loss, were similar to TECD syndrome. Additionally, it was shown that corticosteroid therapy does not prevent corneal endothelial loss in TECD syndrome, as was the same in our case.…”

Section: Discussionsupporting

confidence: 61%

Corneal Injury by Anti-Misting Agent in Swim Goggles

Peng

Chen

Hsu

et al. 2006

Cornea

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Section: Discussionsupporting

confidence: 61%

Corneal Injury by Anti-Misting Agent in Swim Goggles

Peng

Chen

Hsu

et al. 2006

Cornea

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“…Some of the earliest reported cases of TASS described as ''sterile hypopyon endophthalmitis'' were due to toxic detergent residues on reusable ocular instruments from ultrasonic cleaning solutions, heat-stable cleaning detergents, or cleaning or finishing compounds on IOLs. 3,34,35 Detergent residues on ophthalmic surgical instruments have been reported to cause more localized anterior segment toxicity; this syndrome has been referred to as TECDS. Breebaart et al 13 describe severe toxic endothelial cell destruction of the cornea following extracapsular cataract surgery from detergent residues found on reusable cannulas.…”

Section: Intraocular Anestheticsmentioning

confidence: 99%

Toxic anterior segment syndrome

Mamalis

Edelhauser²,

Dawson³

et al. 2006

Journal of Cataract and Refractive Surgery

270

192

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Toxic anterior segment syndrome (TASS) is a sterile postoperative inflammatory reaction caused by a noninfectious substance that enters the anterior segment, resulting in toxic damage to intraocular tissues. The process typically starts 12 to 48 hours after cataract/anterior segment surgery, is limited to the anterior segment of the eye, is always Gram stain and culture negative, and usually improves with steroid treatment. The primary differential diagnosis is infectious endophthalmitis. Review of the literature indicates that possible causes of TASS include intraocular solutions with inappropriate chemical composition, concentration, pH, or osmolality; preservatives; denatured ophthalmic viscosurgical devices; enzymatic detergents; bacterial endotoxin; oxidized metal deposits and residues; and factors related to intraocular lenses such as residues from polishing or sterilizing compounds. An outbreak of TASS is an environmental and toxic control issue that requires complete analysis of all medications and fluids used during surgery, as well as complete review of operating room and sterilization protocols.

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“…JG-B staining was used in a study on the role of the mitochondria in the development of the visual cells of chick embryos (Elfvin 1953). Until then the JG-B technique was only applied to test corneal viability Nuyts et al 1990). JG-B may be seen as a general biomass stain with high affinity to cell nuclei (Lillie 1977).…”

Section: Discussionmentioning

confidence: 99%

Comparative Measurement ofIn VitroParaquat and Aflatoxin B1 Cytotoxicity Using Three Different Cytotoxicity Assays in Pheochromocytoma Cells (PC-12)

Mohammadi-Bardbori

Nejati

Esmaeili

et al. 2008

Toxicology Mechanisms and Methods

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Among the herbicides and mycotoxins, paraquat (PQ) and aflatoxin B1 (AFB1) are highly cytotoxic. In this study the toxicity of PQ and AFB1 in the cultured cell were determined using MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide], JG-B (Janus green B), and NR (neutral red) assay by multiwell scanning spectophotometry. JG-B was used not only for the vital staining of mitochondria but also for viability assay and was compared to MTT and NR assay. Various concentrations of paraquat (0.1 mM to 100 mM) and AFB1 (0.001 nM to 10 nM) on the PC-12 cells were investigated. The 50% lethal concentration of toxins (LC50) were determined for PQ (7.70 +/- 2.50, 3.67 +/- 1.53, 4.85 +/- 2.44) and AFB1 (0.16 +/- 0.01, 0.13 +/- 0.04, 0.14 +/- 0.02) as determined by these methods (JG-B, NR, and MTT, respectively). A significant correlation was found among the JG-B and MTT using PQ (r(2) = 0.99, p < 0.05) and significant correlation was also found among the three methods (r(2) = 0.95, 0.93, and 0.92, p < 0.05) using AFB1. No significant correlation was found between JG-B and MTT with NR (r(2) = 0.34 and 0.35, p < 0.05, respectively) using PQ. These results suggest that both methods (MTT assay and JG-B assay) are reliable and are comparable for determining the cytotoxicity. It is concluded that the JG-B assay may be preferable to MTT assay methods because of its simplicity, low cost, sensitivity, and objectivity; in addition, this method takes little time to be done.

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Toxic Effects of Detergents on the Corneal Endothelium

Cited by 50 publications

References 16 publications

Corneal Injury by Anti-Misting Agent in Swim Goggles

Corneal Injury by Anti-Misting Agent in Swim Goggles

Toxic anterior segment syndrome

Comparative Measurement ofIn VitroParaquat and Aflatoxin B1 Cytotoxicity Using Three Different Cytotoxicity Assays in Pheochromocytoma Cells (PC-12)

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