Toxic effects of fludioxonil on the growth, photosynthetic activity, oxidative stress, cell morphology, apoptosis, and metabolism of Chlorella vulgaris

Liu, Xiang; Wang, Xueting; Zhang, Fengwen; Yao, Xiangfeng; Qiao, Zhihua; Deng, Jiahui; Jiao, Qin; Gong, Luo

doi:10.1016/j.scitotenv.2022.156069

Cited by 20 publications

(3 citation statements)

References 96 publications

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“…Heavy metals may induce neurotoxicity, genotoxicity, and oxidative stress by disrupting prooxidant and antioxidant homeostasis ( 20 ). Fludioxonil, a phenylpyrrole fungicide, was recently reported to induce oxidative stress in various organisms ( 21 – 23 ). Hence, we next investigated the effect of MET14 deletion on intracellular glutathione levels.…”

Section: Resultsmentioning

confidence: 99%

Adenylyl-Sulfate Kinase (Met14)-Dependent Cysteine and Methionine Biosynthesis Pathways Contribute Distinctively to Pathobiological Processes in Cryptococcus neoformans

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Adenylyl-Sulfate Kinase (Met14)-Dependent Cysteine and Methionine Biosynthesis Pathways Contribute Distinctively to Pathobiological Processes in Cryptococcus neoformans

et al. 2023

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“…Alternatively, synthetic fludioxonil, a structurally similar chemical with increased photostability in comparison to PRN and a high antifungal activity, is currently commercially available as a synthetic non-systemic surface fungicide for seed and foliar treatment of plants [ 21 ]. With long-term use of fludioxonil, it has been reported to cause a high toxicity to aquatic organisms, acts as a potential endocrine disruptor in human cell lines, and gives rise to appearance of resistant field strains [ 4 , 12 , 17 , 33 , 54 , 58 , 63 ]. Taking into account food and environmental health risks, the European Food Safety Authority has established maximum residue levels for fludioxonil in various crops, vegetables, and fruits [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum

Putri,

Jung,

Lee

et al. 2024

Microb Cell Fact

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Aminopyrrolnitrin (APRN), a natural halogenated phenylpyrrole derivative (HPD), has strong antifungal and antiparasitic activities. Additionally, it showed 2.8-fold increased photostability compared to pyrrolnitrin, a commercially available HPD with antimicrobial activity. For microbial production of APRN, we first engineered anthranilate phosphoribosyltransferase encoded by trpD from Corynebacterium glutamicum, resulting in a TrpDA162D mutation that exhibits feedback-resistant against l-tryptophan and higher substrate affinity compared to wild-type TrpD. Plasmid-borne expression of trpDA162D in C. glutamicum TP851 strain with two copies of trpDA162D in the genome led to the production of 3.1 g/L l-tryptophan in flask culture. Subsequent step for l-tryptophan chlorination into 7-chloro-l-tryptophan was achieved by introducing diverse sources of genes encoding tryptophan 7-halogenase (PrnA or RebH) and flavin reductase (Fre, PrnF, or RebF). The combined expression of prnA from Serratia grimesii or Serratia plymuthica with flavin reductase gene from Escherichia coli, Pseudomonas fluorescens, or Lechevalieria aerocolonigenes yielded higher production of 7-chloro-l-tryptophan in comparison to other sets of two-component systems. In the next step, production of putative monodechloroaminopyrrolnitrin (MDAP) from 7-chloro-l-tryptophan was achieved through the expression of prnB encoding MDAP synthase from S. plymuthica or P. fluorescens. Finally, an artificial APRN biosynthetic pathway was constructed by simultaneously expressing genes coding for tryptophan 7-halogenase, flavin reductase, MDAP synthase, and MDAP halogenase (PrnC) from different microbial sources within the l-tryptophan-producing TP851 strain. As prnC from S. grimesii or S. plymuthica was introduced into the host strain, which carried plasmids expressing prnA from S. plymuthica, fre from E. coli, and prnB from S. plymuthica, APN3639 and APN3638 accumulated 29.5 mg/L and 28.1 mg/L of APRN in the culture broth. This study represents the first report on the fermentative APRN production by metabolically engineered C. glutamicum.

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“…In order to keep ROS and radical generation at a reasonably steady level and to protect organisms from oxidative stress, antioxidant enzymes (such as SOD and CAT) could selectively catalyze the transformation of O 2 • and H 2 O 2 into oxygen and water. As a result, the antioxidant enzymes are considered the most sensitive indicators for a wide variety of environmental stressors(Hu et al 2015a, Liu et al 2022. With an increase in oxidative stress, the activity of the antioxidant enzymes would also increase(Finaud et al 2006).…”

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confidence: 99%

Comparative ecotoxicity of graphene, functionalized multiwalled CNT and their mixture in freshwater microalgae, Scenedesmus obliquus: Analysing the role of oxidative stress

Das

Giri

Wadhwa

et al. 2023

Preprint

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Due to their remarkable properties, the applications of carbon-based nanomaterials (CNMs) such as graphene and functionalized multi-walled carbon nanotubes (f-MWCNTs) are increasing. These CNMs can enter the freshwater environment via numerous routes potentially exposing various organisms. The current study assesses the effects of graphene, f-MWCNTs, and their binary mixture on the freshwater algal species Scenedesmus obliquus. The concentration for the individual materials was kept at 1 mg L− 1, while for the mixture, both graphene and f-MWCNTs were taken at 0.5 mg L− 1each. Both the CNMs caused a decrease in cell viability, esterase activity, and photosynthetic efficiency in the cells. The cytotoxic effects were accompanied by an increase in hydroxyl and superoxide radical generation, lipid peroxidation, antioxidant enzyme activity (catalase and superoxide dismutase), and mitochondrial membrane potential. Graphene was more toxic compared to f-MWCNTs. The binary mixture of the pollutants demonstrated a synergistic enhancement of the toxic potential. The oxidative stress generation played a critical role in toxicity responses, as noted by a strong correlation between the physiological parameters and the biomarkers of oxidative stress. The outcomes from this study emphasize the significance of considering the combined effects of various CNMs as part of a thorough evaluation of ecotoxicity in freshwater organisms.

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Toxic effects of fludioxonil on the growth, photosynthetic activity, oxidative stress, cell morphology, apoptosis, and metabolism of Chlorella vulgaris

Cited by 20 publications

References 96 publications

Adenylyl-Sulfate Kinase (Met14)-Dependent Cysteine and Methionine Biosynthesis Pathways Contribute Distinctively to Pathobiological Processes in Cryptococcus neoformans

Adenylyl-Sulfate Kinase (Met14)-Dependent Cysteine and Methionine Biosynthesis Pathways Contribute Distinctively to Pathobiological Processes in Cryptococcus neoformans

Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum

Comparative ecotoxicity of graphene, functionalized multiwalled CNT and their mixture in freshwater microalgae, Scenedesmus obliquus: Analysing the role of oxidative stress

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