TOXIC EFFECTS OF LIPOPOLYSACCHARIDE FROM BACTEROIDES INTERMEDIUS AND ESCHERICHIA COLI ASSESSED IN THE PRE‐IMPLANTATION MOUSE EMBRYO CULTURE SYSTEM

Abstract

A comparative study on the toxic effects of endotoxin (lipopolysaccharide, LPS) from the strictly anaerobic Bacteroides intermedius BM1 and from Escherichia coli 055 was performed. Pre‐implantation mouse embryos at the 2‐cell stage from LPS responder (C57BL/6J) and low responder (C3H/Hej) mouse strrains were exposed to the endotoxins, and development was observed during 72 h in vitro. Toxic effects of both endotoxins on embryos from both mouse strains were demonstrated. B. intermedius LPS was less toxic than E. coli LPS. The C3H/Hej embryos were more susceptible to endotoxins than the C57BL/6J embryos. A biphasic dose‐response relationship was observed, as 100 μg/ml LPS was less embryotoxic than 1, 10 or 500 μg/ml, suggesting more complex mechanisms of effect than non‐specific cell toxicity.

Proteomic Characterization of Histotroph during the Preimplantation Phase of the Estrous Cycle in Cattle

“…Tissue nonspecific alkaline phosphatase (TNAP), a member of the alkaline phosphatase family reported to detoxify LPS, has been suggested as a possible therapeutic against LPS mediated diseases. , Gram-negative bacteria, the source of LPS, are the most common bacteria isolated from bovine uterine infections . In addition, both elevated systemic and in vitro LPS concentrations have demonstrated negative effects on embryo development. , Therefore, TNAP may be involved in maintaining uterine health (a major determinant of reproductive success in cattle) and embryo well being.…”

“…110 In addition, both elevated systemic and in vitro LPS concentrations have demonstrated negative effects on embryo development. 111,112 Therefore, TNAP may be involved in maintaining uterine health (a major determinant of reproductive success in cattle 113 ) and embryo well being.…”