Toxic effects of mancozeb containing formulations and neemix pesticides on kidney function and ultrastructure of albino rats

Abstract

Chronic effects of mancozeb contaning formulations (Anadol, Blanko, Deltathin, Tazolin) and Nemic pesticides on kidney function and ultrastructure of albino rats were studied. Thirty six male albino rats weighing 200-220 g were housed in the laboratory under natural condition and received normal commercial ration and water ad-lib then subdivided into six groups, six animals each: control, Anadol, Blanko, Deltathin, Nemic and Tazolin groups. Animals of each pesticide groups were received 25% of the LD 50 of the corresponding pesticide once weekly for eight weeks using stomach tube.Results revealed significant decrease (p < 0.05) in serum total protein and albumen of all treated groups compared to control group. The lowest value of total protein (6.91 + 0.51 g / dl) and albumen (3.86 + 0.12 g / dl) was recorded for Tazolin group. In the other hand serum urea and uric acid were significantly increased (P < 0.05) in all treated groups compared to control group. Tazolin group comes on the top record (42.34 + 5.65 mg / dl for urea and 8.44 + 1.02 mg / dl for uric acid).A significantly elevated serum urea and uric acid concentrations accompanied with decreased total protein and albumen levels usually indicates impaired glomerular function.Results of the kidney ultrastructure of the different treated groups shows marked alterations compared to control group. Renal glomerulus, shows, degenerated basal lamina, endothelial cells, podocytes and degraded podocyte pedicels. Proximal convoluted tubule with fragmented brush border, plasmalemma, large number of vacuoles, dense granulation, mitochondria and lysosomes are seen specially in Tazolin treated group. Distal convoluted tubule with, plasmalemma, large number of vacuoles, dense granulation, mitochondria and lysosomes and secreted granules was also seen in all treated groups. It is concluded that the contamination produced as the result of using mancozeb containing pesticide and Nemic pesticide for long time may have severe toxic effect on kidney function and ultrastructure.