Toxic effects of pyrethroids in tadpoles of Physalaemus gracilis (Anura: Leptodactylidae)

Vanzetto, Guilherme Victor; Slaviero, Jéssica G.; Sturza, Paola F.; Rutkoski, Camila Fátima; Macagnan, Natani; Kolcenti, Cassiane; Hartmann, Paulo Afonso; Ferreira, Cláudia Maris; Hartmann, Marília Teresinha

doi:10.1007/s10646-019-02115-0

Cited by 17 publications

(6 citation statements)

References 60 publications

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“…We know of no other study that examined the long-term effects of cypermethrin- or deltamethrin exposure applied after metamorphosis on the survival of amphibians. However, experiments reporting toxicity of various pyrethroids on aquatic life stages of amphibians mostly applied chronic exposure (several days) and/or very high concentrations of the chemicals (Agostini et al, 2009; Berrill et al, 1993; Biga and Blaustein, 2013; Macagnan et al, 2017; Vanzetto et al, 2019b). Experiments regarding the survival of metamorphic amphibians exposed to pesticides are scarce, also rely on chronic exposures (Jones et al, 2017; Zhou et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In their case, exposure to pyrethroids can happen both in aquatic (as embryos or larvae) and in terrestrial environments (as juveniles and adults). Most ecotoxicological studies that examined the malign effects of pyrethroids on amphibians concentrated on aquatic life stages (Agostini et al, 2020(Agostini et al, , 2009Berrill et al, 1993;Biga and Blaustein, 2013;David et al, 2012;Macagnan et al, 2017;Nataraj and Krishnamurthy, 2012;Vanzetto et al, 2019a). Cypermethrin and deltamethrin, two of the most heavily used pyrethroids, are often used for mosquito control, and the time and location of spraying can overlap with the emergence of juvenile amphibians from water bodies.…”

Section: Introductionmentioning

confidence: 99%

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Effects of mild winter conditions and two pyrethroid insecticides on the development and survival of juvenile common toads (Bufo bufo)

Kásler,

Bókony,

Mikó

et al. 2023

Preprint

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Combined effects of pollutants and climate change can have long-term negative effects on Earth's biodiversity. We tested how amphibians are affected by exposure to pesticides around metamorphosis in combination with current winter conditions or those projected to become the norm by 2100. We applied two pyrethroid insecticides, cypermethrin or deltamethrin, to newly metamorphosed common toads (Bufo bufo) via spraying, thereafter reared animals in outdoor enclosures, and finally exposed them either to a long&cold winter (1.5 degrees C for 91 days) or to a short&mild one (4.5 degrees C for 61 days). After hibernation, we examined changes in body mass, size and gross morphology of parotoid glands and visceral organs, and determined the phenotypic sex of the surviving toadlets. In the long&cold winter scenario, we observed high mortality rate, regardless of the chemical treatment (control: 82%, cypermethrin: 78%, deltamethrin: 70%). At the same time, mortality was significantly lower in the short&mild winter scenario (control: 33%, cypermethrin: 41%, deltamethrin: 26%). The decrease in body mass during overwintering did not differ among treatment groups. Our results contradict the hypothesis of sex-biased mortality caused by chemical pollutants, harsh or mild winter conditions or by their combination. No significant deformations of the internal organs were found in either group. Our results suggest that winter conditions becoming ever milder in the temperate zone may prove beneficial for amphibian overwinter survival, and that exposure to the tested pyrethroids at ecologically relevant concentrations after metamorphosis does not interfere with the fitness of juvenile common toads.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of mild winter conditions and two pyrethroid insecticides on the development and survival of juvenile common toads (Bufo bufo)

Kásler,

Bókony,

Mikó

et al. 2023

Preprint

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“…Lower concentrations were used if prior amphibian data on similar pesticides suggested higher toxicity or if complete mortality occurred at 1 mg/L in the first test run. For example, previous research on pyrethroids found significant toxicity to amphibians (e.g., Vanzetto et al, 2019). If mortality did not occur at 100 mg/L the definitive tests were not performed and the LC50 was reported as > 100 mg/L.…”

Section: Toxicity Testing and Pesticidesmentioning

confidence: 99%

Comparative acute toxicity of pesticides to tadpoles of a tropical anuran (<em>Epipedobates anthonyi</em>), a North American native anuran (<em>Lithobates sphenocephalus</em>) and a standard fish species

Weir,

Weltje

2024

bah

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Global amphibian declines have the highest incidence in tropical regions, but most of the ecotoxicological data on amphibians is collected on temperate northern hemisphere anuran species. We tested the hypothesis that tropical anuran larvae (Epipedobates anthonyi) would be more sensitive to pesticides than a North American native species (Lithobates sphenocephalus). For 12 pesticides, 96-hr range-finding acute toxicity tests were conducted to determine if mortality occurred at environmentally relevant levels. Based on those studies, two substances were selected for additional time-to-event analyses in both species as well as median lethal concentration (LC50) calculations. Time-to-event results indicated that the two species appear to be roughly equivalent in their sensitivity to the two tested pesticides. Significant differences between species were not consistent across concentrations for either the insecticide terbufos or the herbicide pendimethalin. The utility of LC50 data was mixed with one LC50 providing an arbitrarily large standard error around the LC50 precluding informative comparisons across species. However, standard LC50 methods allowed data collection that continues to contribute to our understanding of the protectiveness of fish as surrogates for anuran larvae. While our data set is limited, it appears that testing temperate species would be protective for tropical species in ecological risk assessments. Our data also support the continued use of fish as surrogates for amphibian larvae as none of the species were more sensitive to the tested pesticides than rainbow trout (Oncorhynchus mykiss), the standard sensitive fish species used for acute toxicity testing.

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“…Це може проявлятися у відсутності або частковій відсутності зубців, нижньої та верхньої щелепи, як, наприклад, у пуголовків південноамериканської жаби Ph. gracilis після 168 годин впливу циперметрину в концентраціях 0,01 та 0,03 мг/л (Vanzetto et al, 2019). Циперметрин викликає анізохромію та збільшує частку незрілих еритроцитів у крові амфібій (Attademo et al, 2011).…”

Section: вплив на морфологічні показники тіла та метаморфозunclassified

Biomarkers of the influence of pyrethroids and neonicotinoids on amphibian larvae

Gasso

Yermolenko

Bobyliov

et al. 2020

ean

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Currently, most amphibian populations in the world exist under the influence of numerous stress factors. Among them, the main factors that affect almost all terrestrial animals, namely, the fragmentation of habitats, environmental pollution and anthropic transformation of landscapes. Moreover, those factors are joined by negative causes that affect only amphibians – specific viral and fungal infections: ranaviruses (Ambystoma tigrinum virus (ATV), Bohle iridovirus (BIV), and frog virus 3) (also dangerous for some reptiles) and chytrid fungi (Batrachochytrium dendrobatidis and B. salamandrivorans). All these factors are one of the main reasons for the current global decline of amphibian populations in the world. In today's world, agricultural chemicals are one of the most important in terms of toxicity, environmental emissions and total area of impact. Among them, a significant proportion are formed by synthetic insecticides, which include pyrethroids and neonicotinoids. Pollution by these substances has a negative impact on amphibian populations, despite the relatively short period of their life in the environment. The vast majority of studies on the effects of pyrethroid and neonicotinoid insecticides were made in laboratory experiments with the larval stages of tailless amphibians. Tadpoles are easy to get in sufficient quantities and kept in the laboratory. Cypermethrin reduces the viability of tadpoles and causes precocious metamorphosis of survived larvae. However, in a combination with other pesticides, it delays metamorphosis. In addition, embryos were more resistant to pyrethroids than tadpoles. Pyrethroid pesticides cause spasms indicating adverse neurological effects. Formation of oral apparatus abnormalities in tadpoles, anisochromasia and increasing number of immature erythrocytes are also caused by pyrethroids. Neonicotinoids show similar effects. Under the action of imidacloprid the erythrocytes are also disturbed that is showed up in the DNA damage and micronuclei formation. Neurological disorders are manifested in the ability to perceive or respond to a predator, disorientation, erratic movement and loss of balance. Pyrethroids and neonicotinoids have been shown cause a variety of disorders: increase mortality and reduce survival of tadpoles; have a teratogenic effect and affect the metamorphosis and morphological parameters of amphibians; change many biochemical parameters that characterize the protein metabolism and oxidative stress; have genotoxic effects and affect the state of the nervous system and animal behaviour. Most of these parameters are proposed for use as biomarkers of pesticide intoxication.

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Toxic effects of pyrethroids in tadpoles of Physalaemus gracilis (Anura: Leptodactylidae)

Cited by 17 publications

References 60 publications

Effects of mild winter conditions and two pyrethroid insecticides on the development and survival of juvenile common toads (Bufo bufo)

Effects of mild winter conditions and two pyrethroid insecticides on the development and survival of juvenile common toads (Bufo bufo)

Comparative acute toxicity of pesticides to tadpoles of a tropical anuran (<em>Epipedobates anthonyi</em>), a North American native anuran (<em>Lithobates sphenocephalus</em>) and a standard fish species

Biomarkers of the influence of pyrethroids and neonicotinoids on amphibian larvae

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