Toxic effects of sodium fluoride on cell proliferation and apoptosis of Leydig cells from young mice

Song, Guo Hua; Wang, Rui Li; Chen, Zhao Yang; Zhang, Bin; Wang, Hailong; Liu, Mao Lin; Gao, Junfang; Yan, Xiao

doi:10.1007/s13105-014-0344-1

Cited by 48 publications

(21 citation statements)

References 19 publications

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“…Fluorine is one of the essential trace elements for human body [2]. Moderate levels of fluorine or fluoride ingestion can decrease the incidence of dental caries and promote the development of bones, but there are a number of adverse effects on human health with fluorine or fluoride chronic ingestion at high doses [3].…”

Section: Introductionmentioning

confidence: 99%

Sodium fluoride causes oxidative stress and apoptosis in the mouse liver

Luo

Cui

et al. 2017

Aging

112

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The current study was conducted to investigate the effect of sodium fluoride (NaF) on the oxidative stress and apoptosis as well as their relationship in the mouse liver by using methods of flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), western blot, biochemistry and experimental pathology. 240 four-week-old ICR mice were randomly divided into 4 groups and exposed to different concentration of NaF (0 mg/kg, 12 mg/kg, 24 mg/kg and 48 mg/kg) for a period of 42 days. The results showed that NaF caused oxidative stress and apoptosis. NaF-caused oxidative stress was accompanied by increasing reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and decreasing mRNA expression levels and activities of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GSH-PX) and glutathione-s-transferase (GST). NaF induced apoptosis via tumor necrosis factor recpter-1 (TNF-R1) signaling pathway, which was characterized by significantly increasing mRNA and protein expression levels of TNF-R1, Fas associated death domain (FADD), TNFR-associated death domain (TRADD), cysteine aspartate specific protease-8 (caspase-8) and cysteine aspartate specific protease-3 (caspase-3) in dose- and time-dependent manner. Oxidative stress is involved in the process of apoptotic occurrence, and can be triggered by promoting ROS production and reducing antioxidant function. NaF-caused oxidative stress and apoptosis finally impaired hepatic function, which was strongly supported by the histopathological lesions and increased serum alanine amino transferase (ALT), aspartic acid transferase (AST), alkaline phosphatase (AKP) activities and TBIL contents.

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Section: Introductionmentioning

confidence: 99%

Sodium fluoride causes oxidative stress and apoptosis in the mouse liver

Luo

Cui

et al. 2017

Aging

112

View full text Add to dashboard Cite

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“…Fluorine is one of the essential trace elements for human health [1]. However, excessive fluoride intake can cause serious tissue damage and lead to multiple organ dis-function [2, 3], which depends not only on the concentration and exposed duration [4, 5], but also on the absorption capacity, age, and nutritional status of the individual [6].…”

Section: Introductionmentioning

confidence: 99%

Sodium fluoride induces apoptosis in cultured splenic lymphocytes from mice

et al. 2016

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Though fluorine has been shown to induce apoptosis in immune organs in vivo, there has no report on fluoride-induced apoptosis in the cultured lymphocytes. Therefore, this study was conducted with objective of investigating apoptosis induced by sodium fluoride (NaF) and the mechanism behind that in the cultured splenic lymphocytes by flow cytometry, western blot and Hoechst 33258 staining. The splenic lymphocytes were isolated from 3 weeks old male ICR mice and exposed to NaF (0, 100, 200, and 400 μmol/L) in vitro for 24 and 48 h. When compared to control group, flow cytometry assay and Hoechst 33258 staining showed that NaF induced lymphocytes apoptosis, which was promoted by decrease of mitochondria transmembrane potential, up-regulation of Bax, Bak, Fas, FasL, caspase 9, caspase 8, caspase 7, caspase 6 and caspase 3 protein expression (P < 0.05 or P <0.01), and down-regulation of Bcl-2 and Bcl-xL protein expression (P <0.05 or P <0.01). The above-mentioned data suggested that NaF-induced apoptosis in splenic lymphocytes could be mediated by mitochondrial and death receptor pathways.

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“…In mammalian cells, caspases are activated by the intrinsic or the extrinsic apoptotic pathway. The intrinsic pathway is activated by various stimuli and acts through the mitochondria [25,26]. Previous studies reported apoptosis in the liver of rat treated by fluoride, such as alteration of oxidative stress, DNA damage, apoptosis and cell cycle changes induced by fluoride in rat hepatocytes, and Fas/FasL expressions in the liver of fluoride-exposed rats [27,28].…”

Section: Introductionmentioning

confidence: 99%

Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

Song

Huang

Gao

et al. 2015

Biol Trace Elem Res

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Fluoride compounds are abundant and widely distributed in the environment at a variety of concentrations. Further, fluoride induces toxic effects in target organs such as the liver. In this study, we investigated liver histopathology, DNA damage, apoptosis, and the mRNA and protein expressions of caspase-3 and -9 in the rat livers by administering varying concentrations of fluoride (0, 50, 100, 200 mg/L ) for 120 days. The results showed fluoride-induced morphological changes and significantly increased apoptosis and DNA damage in rats exposed to fluoride, especially in response to higher doses. The immunohistochemical and qRT-PCR results indicated that caspase-3, caspase-9 protein positive expression and mRNA relative expression enhanced with increasing NaF concentration. In summary, our findings suggest that chronic exposure to fluoride causes damages to liver histopathology and leads to liver apoptosis through caspase-mediated pathways.

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Toxic effects of sodium fluoride on cell proliferation and apoptosis of Leydig cells from young mice

Cited by 48 publications

References 19 publications

Sodium fluoride causes oxidative stress and apoptosis in the mouse liver

Sodium fluoride causes oxidative stress and apoptosis in the mouse liver

Sodium fluoride induces apoptosis in cultured splenic lymphocytes from mice

Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

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