Toxic effects of the non-steroidal anti-inflammatory drug diclofenac

Triebskorn, Rita; Casper, Heidi; Heyd, Allen; Eikemper, R; Köhler, Heinz‐R.; Schwaiger, Jens

doi:10.1016/j.aquatox.2004.03.015

Cited by 373 publications

(69 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Germany, for example, the yearly consumption exceeds 80 tons [8]. Depending on the efficiency of the WWTP, the parent drug and metabolites enter the environment, followed by bioaccumulation and impairment of aquatic wildlife like mussels and fish, possibly [9][10][11][12][13][14][15]. The most spectacular observation has been reported from South Asia that veterinary use of DCF is the main cause of catastrophic population decline of three species of Gyps vultures since the mid-1990s [16][17][18].…”

Section: Introductionmentioning

confidence: 95%

“…In surface water, DCF was found at concentrations up to 0.6 μg/L. One subject of current research is whether environmental exposure to DCF at relevant concentration in freshwater can affect certain species, e.g., fish [9][10][11][12][13][14][15]. Recently, DCF and the major human metabolites 4'OH-DCF, 5-OH-DCF, and DCF-1-O-acyl glucuronide (DCF-GLU) were quantified in WWTP influents and effluents [22].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid analysis of diclofenac in freshwater and wastewater by a monoclonal antibody-based highly sensitive ELISA

et al. 2015

View full text Add to dashboard Cite

The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is found worldwide in the aqueous environment. Therefore, it has raised increased public concern on potential long-term impact on human health and wildlife. The importance of DCF has been emphasized by the European Union recently by including this pharmaceutical in the first watch list of priority hazardous substances in order to gather Union-wide monitoring data. Rapid and cheap methods of analysis are therefore required for fresh and wastewater monitoring with high sample load. Here, for the first time, well-characterized monoclonal antibodies (mAbs) against DCF were generated and a highly sensitive ELISA developed. The best antibody (mAb 12G5) is highly affine (KD = 1.5 × 10(-10) M), stable to potential matrix interferences such as pH value (pH range 5.2-9.2), calcium ion concentration (up to 75 mg/L), and humic acid content (up to 20 mg/L). The limit of detection (LOD, S/N = 3) and IC50 of the ELISA calibration curve were 7.8 and 44 ng/L, respectively. The working range was defined between 11 and 180 ng/L. On average, about 10 % cross-reactivity (CR) was found for DCF metabolites 5-OH-DCF, 4'-OH-DCF, and DCF-acyl glucuronide, but other structurally related NSAIDs showed binding <1 % compared to the parent compound. While DCF concentrations at the low ppt range were measured in river and lake water, higher values of 2.9 and 2.1 μg/L were found in wastewater influents and effluents, respectively. These results could be confirmed by solid phase extraction combined with LC-MS.

show abstract

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Rapid analysis of diclofenac in freshwater and wastewater by a monoclonal antibody-based highly sensitive ELISA

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Based on this it is considered justified to base the PNEC not on acute, but rather on chronic data. As the data from Hoeger et al and Schwaiger and co-workers illustrate, [23][24][25] neither present an acute nor a chronic exposure scenario but rather a subchronic situation, an AF of 100 would most likely be an acceptable calculation scenario. A PNEC of 0.005 µg/L (NOEC/100) would therefore be derived as a conservative scenario.…”

Section: Pnecmentioning

confidence: 99%

“…Indeed, as many enzyme/receptor systems are highly conserved across the different phyla, specific target interactions of given pharmaceuticals in species other than the humans are to be expected, although the dynamically required doses and the array of effects may be different. Indeed, the reported nephrotoxic effects of diclofenac in rodents and other mammals, was also demonstrated to occur in fish [23][24][25] of up to 1.2 µg/L and higher [26]. The no observed effect concentration (NOEC) in the study by Hoeger et al [23] was determined to be 0.5 µg/L for monocyte infiltration/accumulation in livers of brown trout exposed for 21 days, although mild effects were seen in two of six animals in the 0.5 µg/L group.…”

Section: Pnecmentioning

confidence: 99%

Toxicology and Risk Assessment of Pharmaceuticals

Dietrich

Hitzfeld

O'Brien

2006

Organic Pollutants in the Water Cycle

View full text Add to dashboard Cite

“…Consequences of long-term exposure to DCF up to 3 months were also evaluated using freshwater crustaceans (Daphnia magna and Moina macrocopa) and a fish (Oryzias latipes) by Lee et al (2011). The presence of DCF at the concentration of 1 lg/L was reported to damage the liver and kidney cell functions in fish (Triebskorn et al 2004). Besides, Nassef et al (2010) also investigated the toxicity of three kinds of pharmaceuticals, CBM, DCF, and triclosan (TCS), through examining their effects on feeding behavior and swimming speed of adult Japanese medaka fish (O. latipes).…”

Section: Introductionmentioning

confidence: 99%

Removal of typical pharmaceutically active compounds in sewage sludge using mesophilic and thermophilic anaerobic digestion processes

Zhou

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

The removal of four pharmaceutically active compounds, i.e., anti-inflammatory painkiller diclofenac (DCF), lipid-regulating agent clofibric acid (CFA), epilepsy drugs carbamazepine (CBM), and broad-spectrum anti-bacterial agent triclosan (TCS), present in sewage sludge was investigated using anaerobic digestion processes in the mesophilic and thermophilic modes. Sludge retention times (SRTs) were set at 10, 15, and 20 days, respectively, for the mesophilic mode and 7, 15, and 20 days, respectively, for the thermophilic mode. The effective isolation and purification pre-treatment to extract the target compounds from the sewage sludge samples were firstly established, followed by gas chromatographymass spectrometer analysis to identify and quantify them. The removal efficiencies of the target compounds could be raised to a certain extent with the increase on SRTs, especially under the mesophilic condition. The removal of CFA and TCS under thermophilic condition hardly varied when the SRTs were above 15 days. All the compounds could be partly removed from the sewage sludge under the two temperature conditions, particularly TCS that was reduced by about 74 %. Besides, CFA, firstly reported in this study, could be reduced by maximal 65 %. On the whole, the thermophilic mode was more conducive to the removal of CBM and TCS, but did not have a noticeable effect on the removal of CFA. Furthermore, DCF could be better removed in the mesophilic mode. In addition, NH 3 -N accumulation in this measured range might positively affect the removal of the selected compounds, particularly in the mesophilic mode.

show abstract

Toxic effects of the non-steroidal anti-inflammatory drug diclofenac

Cited by 373 publications

References 25 publications

Rapid analysis of diclofenac in freshwater and wastewater by a monoclonal antibody-based highly sensitive ELISA

Rapid analysis of diclofenac in freshwater and wastewater by a monoclonal antibody-based highly sensitive ELISA

Toxicology and Risk Assessment of Pharmaceuticals

Removal of typical pharmaceutically active compounds in sewage sludge using mesophilic and thermophilic anaerobic digestion processes

Contact Info

Product

Resources

About