Toxic Effects of TiO2 NPs on Zebrafish

Tang, Tianle; Zhang, Zhang; Zhu, Xiaopeng

doi:10.3390/ijerph16040523

Cited by 62 publications

(36 citation statements)

References 36 publications

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“…Among the few data available in the literature, D’Agata and co-workers [ 69 ] showed that concentrations of nano-TiO 2 on the order of 10 mg/L resulted in moderate gill DNA damage and hemocyte infiltration. Similarly, many studies on fish have revealed that nano-TiO 2 can induce oxidative stress, cell membrane damage, protein inactivation and chromosome damage [ 70 ]. On the contrary, Della Torre and colleagues [ 37 ], after 96 h exposure to 0.1 mg/L, did not find any genotoxic effect exerted by nano-TiO 2 in mussel gill cells, investigated through Comet assay.…”

Section: Discussionmentioning

confidence: 99%

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

et al. 2021

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Benzo(a)pyrene (B(a)P) is a well-known genotoxic agent, the removal of which from environmental matrices is mandatory, necessitating the application of cleaning strategies that are harmless to human and environmental health. The potential application of nanoparticles (NPs) in the remediation of polluted environments is of increasing interest. Here, specifically designed NPs were selected as being non-genotoxic and able to interact with B(a)P, in order to address the genetic and chromosomal damage it produces. A newly formulated pure anatase nano-titanium (nano-TiO2), a commercial mixture of rutile and anatase, and carbon black-derived hydrophilic NPs (HNP) were applied. Once it had been ascertained that the NPs selected for the work did not induce genotoxicity, marine mussel gill biopsies were exposed in vitro to B(a)P (2 μg/mL), alone and in combination with the selected NPs (50 µg/mL nano-TiO2, 10 µg/mL HNP). DNA primary reversible damage was evaluated by means of the Comet assay. Chromosomal persistent damage was assessed on the basis of micronuclei frequency and nuclear abnormalities by means of the Micronucleus-Cytome assay. Transmission Electron Microscopy (TEM) was performed to investigate the mechanism of action exerted by NPs. Pure Anatase n-TiO2 was found to be the most suitable for our purpose, as it is cyto- and genotoxicity free and able to reduce the genetic and chromosomal damage associated with exposure to B(a)P.

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Section: Discussionmentioning

confidence: 99%

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

et al. 2021

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“…However, ecological life examinations, potential bioaccumulation, and especially the transfer of NPs within the food chain and air remain restricted. Because TiO 2 NPs are inevitable in the production, use, and disposal of waste through the air, soil, and water, the natural environment’s ecological environment has attracted considerable attention both domestically and internationally [ 22 ]. Employees and academic researchers of production industries may experience the highest threat to exposure of these NPs through inhalation and skin penetration [ 11 ].…”

Section: Mass Production and Filthiness Of Tio 2 Nmentioning

confidence: 99%

Toxicological Consequences of Titanium Dioxide Nanoparticles (TiO2NPs) and Their Jeopardy to Human Population

et al. 2021

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Titanium dioxide nanoparticles (TiO 2 NPs) are the most produced nanomaterial for food additives, pigments, photocatalysis, and personal care products. These nanomaterials are at the forefront of rapidly developing indispensable nanotechnology. In all these nanomaterials, titanium dioxide (TiO 2 ) is the most common nanomaterial which is being synthesized for many years. These nanoparticles of TiO 2 are widely used at the commercial level, especially in cosmetic industries. High usage in such a way has increased the toxicological consequences of the human population. Several studies have shown that TiO 2 NPs accumulated after oral exposure or inhalation in the alimentary canal, lungs, heart, liver, spleen, cardiac muscle, and kidneys. Additionally, in mice and rats, they disturb glucose and lipid homeostasis. Moreover, TiO 2 nanoparticles primarily cause adverse reactions by inducing oxidative stress that leads to cell damage, inflammation, genotoxicity, and adverse immune responses. The form and level of destruction are strongly based on the physical and chemical properties of TiO 2 nanoparticles, which administer their reactivity and bioavailability. Studies give indications that TiO 2 NPs cause both DNA strand breaks and chromosomal damages. The effects of genotoxicity do not depend only on particle surface changes, size, and exposure route, but also relies on the duration of exposure. Most of these effects may be because of a very high dose of TiO 2 NPs. Despite increased production and use, epidemiological data for TiO 2 NPs is still missing. This review discusses previous research regarding the impact of TiO 2 NP toxicity on human health and highlights areas that require further understanding in concern of jeopardy to the human population. This review is important to point out areas where extensive research is needed; thus, their possible impact on individual health should be investigated in more details.

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“…Antioxidant enzymes’ gene expression was upregulated to resist the adverse effects of TiO 2 -NPs, which is consistent with the basic defense mechanism of organisms [ 10 ]. However, an excessive amount of TiO 2 -NPs results in an over production of reactive oxygen species (ROS) with oxidative damage in various aquatic models both in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 57%

Adsorption of Cd to TiO2-NPs Forms Low Genotoxic Aggregates in Zebrafish Cells

Mottola

Santonastaso

Iovine

et al. 2021

Cells

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The aquatic environment is involved in the pollutants spreading mechanisms, including nanomaterials and heavy metals. The aims of this study were to assess the in vivo genotoxicity of Cd (1 mg/L) and to investigate the genomic effects generated by its co-exposure with TiO2-NPs (10 µg/L). The study was performed using zebrafish as a model for 5, 7, 14, 21, and 28 days of exposure. The genotoxic potential was assessed by three experimental approaches: DNA integrity, degree of apoptosis, and molecular alterations at the genomic level by genomic template stability (% GTS) calculation. Results showed an increased in DNA damage after Cd exposure with a decrease in % GTS. The co-exposure (TiO2-NPs + Cd) induced a no statistically significant loss of DNA integrity, a reduction of the apoptotic cell percentage and the recovery of genome stability for prolonged exposure days. Characterization and analytical determinations data showed Cd adsorption to TiO2-NPs, which reduced free TiO2-NPs levels. The results of our study suggest that TiO2-NPs could be used for the development of controlled heavy metal bioremediation systems.

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Toxic Effects of TiO2 NPs on Zebrafish

Cited by 62 publications

References 36 publications

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

Toxicological Consequences of Titanium Dioxide Nanoparticles (TiO2NPs) and Their Jeopardy to Human Population

Adsorption of Cd to TiO2-NPs Forms Low Genotoxic Aggregates in Zebrafish Cells

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